P36017 · VPS21_YEAST
- ProteinVacuolar protein sorting-associated protein 21
- GeneVPS21
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids210 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required for protein transport to the vacuole. Involved in two vesicle trafficking steps to the prevacuolar compartment (PVC), regulating the docking of endosomes and Golgi vesicles to the PVC by interacting with PEP7/VAC1 on the PVC membrane and promoting SNARE complex formation (PubMed:10679018, PubMed:8137814).
May be involved in recruiting the MON1-CCZ1 complex to membranes enriched in phosphatidylinositol 3-phosphate (PtdIns[3]P) or other charged lipids, leading to recruitment of YPT7 (PubMed:32391792).
May be involved in recruiting the MON1-CCZ1 complex to membranes enriched in phosphatidylinositol 3-phosphate (PtdIns[3]P) or other charged lipids, leading to recruitment of YPT7 (PubMed:32391792).
Miscellaneous
Present with 8759 molecules/cell in log phase SD medium.
Activity regulation
Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) VPS9 and inactivated by GTPase-activating proteins (GAPs) GYP1 and GYP3.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | early endosome | |
Cellular Component | endocytic vesicle | |
Cellular Component | endomembrane system | |
Cellular Component | endosome membrane | |
Cellular Component | late endosome | |
Cellular Component | mitochondrial outer membrane | |
Cellular Component | mitochondrion | |
Cellular Component | plasma membrane | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Biological Process | endocytosis | |
Biological Process | Golgi to endosome transport | |
Biological Process | late endosome to vacuole transport via multivesicular body sorting pathway | |
Biological Process | multivesicular body assembly | |
Biological Process | protein localization to endosome | |
Biological Process | protein targeting to vacuole | |
Biological Process | reticulophagy | |
Biological Process | vacuole inheritance |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameVacuolar protein sorting-associated protein 21
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP36017
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endosome membrane ; Lipid-anchor
Mitochondrion membrane ; Lipid-anchor
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 21 | Locks VPS21 in its GDP-bound form by abolishing binding to GTP. | ||||
Sequence: S → L or N | ||||||
Mutagenesis | 66 | Abolishes GTP hydrolysis. | ||||
Sequence: Q → L |
Variants
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The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue, lipidation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000121325 | 1-210 | Vacuolar protein sorting-associated protein 21 | |||
Sequence: MNTSVTSIKLVLLGEAAVGKSSIVLRFVSNDFAENKEPTIGAAFLTQRVTINEHTVKFEIWDTAGQERFASLAPMYYRNAQAALVVYDVTKPQSFIKARHWVKELHEQASKDIIIALVGNKIDMLQEGGERKVAREEGEKLAEEKGLLFFETSAKTGENVNDVFLGIGEKIPLKTAEEQNSASNERESNNQRVDLNAANDGTSANSACSC | ||||||
Modified residue | 188 | Phosphoserine | ||||
Sequence: S | ||||||
Lipidation | 208 | S-geranylgeranyl cysteine | ||||
Sequence: C | ||||||
Modified residue | 210 | Cysteine methyl ester | ||||
Sequence: C | ||||||
Lipidation | 210 | S-geranylgeranyl cysteine | ||||
Sequence: C |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts in its active GTP-bound form with PEP7/VAC1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P36017 | GDI1 P39958 | 5 | EBI-29399, EBI-7517 | |
BINARY | P36017 | MRS6 P32864 | 3 | EBI-29399, EBI-14799 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for motif, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 36-44 | Effector region | ||||
Sequence: KEPTIGAAF | ||||||
Region | 176-210 | Disordered | ||||
Sequence: AEEQNSASNERESNNQRVDLNAANDGTSANSACSC |
Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length210
- Mass (Da)23,081
- Last updated1994-06-01 v1
- Checksum34D7DA0090C33F09
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X76173 EMBL· GenBank· DDBJ | CAA53769.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z29338 EMBL· GenBank· DDBJ | CAA82543.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X94335 EMBL· GenBank· DDBJ | CAA64010.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z74997 EMBL· GenBank· DDBJ | CAA99285.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006948 EMBL· GenBank· DDBJ | DAA10866.1 EMBL· GenBank· DDBJ | Genomic DNA |