P36017 · VPS21_YEAST

Function

function

Required for protein transport to the vacuole. Involved in two vesicle trafficking steps to the prevacuolar compartment (PVC), regulating the docking of endosomes and Golgi vesicles to the PVC by interacting with PEP7/VAC1 on the PVC membrane and promoting SNARE complex formation (PubMed:10679018, PubMed:8137814).
May be involved in recruiting the MON1-CCZ1 complex to membranes enriched in phosphatidylinositol 3-phosphate (PtdIns[3]P) or other charged lipids, leading to recruitment of YPT7 (PubMed:32391792).

Miscellaneous

Present with 8759 molecules/cell in log phase SD medium.

Activity regulation

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) VPS9 and inactivated by GTPase-activating proteins (GAPs) GYP1 and GYP3.

Features

Showing features for binding site.

121020406080100120140160180200
TypeIDPosition(s)Description
Binding site14-21GTP (UniProtKB | ChEBI)
Binding site62-66GTP (UniProtKB | ChEBI)
Binding site120-123GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentearly endosome
Cellular Componentendocytic vesicle
Cellular Componentendomembrane system
Cellular Componentendosome membrane
Cellular Componentlate endosome
Cellular Componentmitochondrial outer membrane
Cellular Componentmitochondrion
Cellular Componentplasma membrane
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Biological Processendocytosis
Biological ProcessGolgi to endosome transport
Biological Processlate endosome to vacuole transport via multivesicular body sorting pathway
Biological Processmultivesicular body assembly
Biological Processprotein localization to endosome
Biological Processprotein targeting to vacuole
Biological Processreticulophagy
Biological Processvacuole inheritance

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Vacuolar protein sorting-associated protein 21
  • Alternative names
    • GTP-binding protein YPT51
    • Vacuolar protein-targeting protein 12

Gene names

    • Name
      VPS21
    • Synonyms
      VPS12, VPT12, YPT21, YPT51
    • ORF names
      YOR3154C
    • Ordered locus names
      YOR089C

Organism names

Accessions

  • Primary accession
    P36017
  • Secondary accessions
    • D6W2F0

Proteomes

Organism-specific databases

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis21Locks VPS21 in its GDP-bound form by abolishing binding to GTP.
Mutagenesis66Abolishes GTP hydrolysis.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue, lipidation.

TypeIDPosition(s)Description
ChainPRO_00001213251-210Vacuolar protein sorting-associated protein 21
Modified residue188Phosphoserine
Lipidation208S-geranylgeranyl cysteine
Modified residue210Cysteine methyl ester
Lipidation210S-geranylgeranyl cysteine

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Interacts in its active GTP-bound form with PEP7/VAC1.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P36017GDI1 P399585EBI-29399, EBI-7517
BINARY P36017MRS6 P328643EBI-29399, EBI-14799

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for motif, region.

TypeIDPosition(s)Description
Motif36-44Effector region
Region176-210Disordered

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    210
  • Mass (Da)
    23,081
  • Last updated
    1994-06-01 v1
  • Checksum
    34D7DA0090C33F09
MNTSVTSIKLVLLGEAAVGKSSIVLRFVSNDFAENKEPTIGAAFLTQRVTINEHTVKFEIWDTAGQERFASLAPMYYRNAQAALVVYDVTKPQSFIKARHWVKELHEQASKDIIIALVGNKIDMLQEGGERKVAREEGEKLAEEKGLLFFETSAKTGENVNDVFLGIGEKIPLKTAEEQNSASNERESNNQRVDLNAANDGTSANSACSC

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X76173
EMBL· GenBank· DDBJ
CAA53769.1
EMBL· GenBank· DDBJ
Genomic DNA
Z29338
EMBL· GenBank· DDBJ
CAA82543.1
EMBL· GenBank· DDBJ
Genomic DNA
X94335
EMBL· GenBank· DDBJ
CAA64010.1
EMBL· GenBank· DDBJ
Genomic DNA
Z74997
EMBL· GenBank· DDBJ
CAA99285.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006948
EMBL· GenBank· DDBJ
DAA10866.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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