P35677 · T2N5_NOSS7

Function

function

A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-TTCGAA-3' and cleaves after T-2.

Catalytic activity

  • Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
    EC:3.1.21.4 (UniProtKB | ENZYME | Rhea)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular Functiontype II site-specific deoxyribonuclease activity
Biological ProcessDNA restriction-modification system

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Type II restriction enzyme NspV
  • EC number
  • Short names
    R.NspV
  • Alternative names
    • Endonuclease NspV
    • Type-2 restriction enzyme NspV

Gene names

    • Name
      nspVR
    • Ordered locus names
      Nos7524_1225

Organism names

Accessions

  • Primary accession
    P35677
  • Secondary accessions
    • K9QQL2

Proteomes

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00000773522-220Type II restriction enzyme NspV

Interaction

Protein-protein interaction databases

Family & Domains

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    220
  • Mass (Da)
    24,664
  • Last updated
    2013-05-29 v2
  • Checksum
    D788B9605C0ED16B
MTILTIEALRTEAAIFSAAESIHPEPLLYGVTDGKAVGTYIEQKFRLYLKEHYEFVQGNSASGIDFPGLLVDVKVTSIRQPQSSCPFKSARQKIFGLGYSLLIFVYDKIDNSTNRTATLNILHTIYVSAERTADFQMTRGIRNILANEGNKDDLIAFMSDRNLPVDEIEAGNVADEILRNPPMQGFLTISNALQWRLQYGRVIERAGQEDGILTVYRNNP

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict175-177in Ref. 1; BAA03538

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D14719
EMBL· GenBank· DDBJ
BAA03538.1
EMBL· GenBank· DDBJ
Genomic DNA
CP003552
EMBL· GenBank· DDBJ
AFY47112.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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