P35560 · KCNJ1_RAT

  • Protein
    ATP-sensitive inward rectifier potassium channel 1
  • Gene
    Kcnj1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.

Activity regulation

Inhibited by WNK3.

Features

Showing features for site, binding site.

139150100150200250300350
TypeIDPosition(s)Description
Site171Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium
Binding site223-230ATP (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentmonoatomic ion channel complex
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular FunctionATP-activated inward rectifier potassium channel activity
Molecular Functioninward rectifier potassium channel activity
Molecular Functionpeptide binding
Molecular Functionphosphatidylinositol-4,5-bisphosphate binding
Molecular Functionpotassium ion binding
Biological Processcellular response to magnesium ion
Biological Processcirculatory system development
Biological Processgene expression
Biological Processkidney development
Biological Processnegative regulation of apoptotic process
Biological Processpost-embryonic development
Biological Processpotassium ion import across plasma membrane
Biological Processpotassium ion transport
Biological Processregulation of monoatomic ion transmembrane transport
Biological Processrenal sodium ion absorption
Biological Processresponse to potassium ion
Biological Processtissue homeostasis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-sensitive inward rectifier potassium channel 1
  • Alternative names
    • ATP-regulated potassium channel ROM-K
    • Inward rectifier K(+) channel Kir1.1
    • KAB-1
    • Potassium channel, inwardly rectifying subfamily J member 1

Gene names

    • Name
      Kcnj1
    • Synonyms
      Romk1

Organism names

  • Taxonomic identifier
  • Strains
    • Sprague-Dawley
    • Wistar
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    P35560
  • Secondary accessions
    • O88639
    • O88640
    • Q9QUR5

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Note: Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane.

Features

Showing features for topological domain, transmembrane, intramembrane.

TypeIDPosition(s)Description
Topological domain1-77Cytoplasmic
Transmembrane78-102Helical; Name=M1
Topological domain103-127Extracellular
Intramembrane128-139Helical; Pore-forming; Name=H5
Intramembrane140-146Pore-forming
Topological domain147-155Extracellular
Transmembrane156-177Helical; Name=M2
Topological domain178-391Cytoplasmic

Keywords

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain, modified residue, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00001549191-391ATP-sensitive inward rectifier potassium channel 1
Modified residue44Phosphoserine; by SGK1
Glycosylation117N-linked (GlcNAc...) asparagine

Post-translational modification

Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Mainly in kidney (renal cortex, medulla and papilla). In the brain, found in the cortex and the hippocampus.

Interaction

Subunit

Interacts with SGK1 and SLC9A3R2/NHERF2.

Protein-protein interaction databases

Chemistry

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif141-146Selectivity filter

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (6)
  • Sequence status
    Complete

This entry describes 6 isoforms produced by Alternative splicing.

P35560-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    391
  • Mass (Da)
    44,963
  • Last updated
    1994-06-01 v1
  • Checksum
    896FD0FD128F85D0
MGASERSVFRVLIRALTERMFKHLRRWFITHIFGRSRQRARLVSKEGRCNIEFGNVDAQSRFIFFVDIWTTVLDLKWRYKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVENINGMTSAFLFSLETQVTIGYGFRFVTEQCATAIFLLIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM

P35560-2

  • Name
    B
  • Synonyms
    ROMK2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

P35560-3

  • Name
    C
  • Synonyms
    ROMK3
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-12: MGASERSVFRVL → MVSELSIPSIPTGVAGLSK

P35560-4

  • Name
    D
  • Synonyms
    1.1, ROMK1.1
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 79-124: YKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVEN → DSSDHRLRIQVCDRTVRHCHFPAYLPVYSWSDHQFLHVWCHFSQDL
    • 125-391: Missing

P35560-5

  • Name
    E
  • Synonyms
    3.1, ROMK3.1
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-12: MGASERSVFRVL → MVSELSIPSIPTGVAGLSK
    • 79-124: YKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVEN → DSSDHRLRIQVCDRTVRHCHFPAYLPVYSWSDHQFLHVWCHFSQDL
    • 125-391: Missing

P35560-6

  • Name
    F
  • Synonyms
    6.1, ROMK6.1
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-19: Missing
    • 79-124: YKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVEN → DSSDHRLRIQVCDRTVRHCHFPAYLPVYSWSDHQFLHVWCHFSQDL
    • 125-391: Missing

Computationally mapped potential isoform sequences

There are 5 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A0G2K298A0A0G2K298_RATKcnj1218
A0A0G2JYW2A0A0G2JYW2_RATKcnj1391
A0A8I6AW52A0A8I6AW52_RATKcnj1372
A0A8I5ZJL6A0A8I5ZJL6_RATKcnj1398
A0A8I5ZLP0A0A8I5ZLP0_RATKcnj1398

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0028001-12in isoform C and isoform E
Alternative sequenceVSP_0027991-19in isoform B and isoform F
Alternative sequenceVSP_00280179-124in isoform D, isoform E and isoform F
Alternative sequenceVSP_002802125-391in isoform D, isoform E and isoform F

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X72341
EMBL· GenBank· DDBJ
CAA51068.1
EMBL· GenBank· DDBJ
mRNA
L29403
EMBL· GenBank· DDBJ
AAA50378.1
EMBL· GenBank· DDBJ
mRNA
S69385
EMBL· GenBank· DDBJ
AAB30553.1
EMBL· GenBank· DDBJ
mRNA
AF081365
EMBL· GenBank· DDBJ
AAC34443.1
EMBL· GenBank· DDBJ
mRNA
AF081366
EMBL· GenBank· DDBJ
AAC34444.1
EMBL· GenBank· DDBJ
mRNA
AF081367
EMBL· GenBank· DDBJ
AAC34445.1
EMBL· GenBank· DDBJ
mRNA
AF081368
EMBL· GenBank· DDBJ
AAC34446.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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