P35560 · KCNJ1_RAT
- ProteinATP-sensitive inward rectifier potassium channel 1
- GeneKcnj1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids391 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This channel is activated by internal ATP and can be blocked by external barium.
Activity regulation
Inhibited by WNK3.
Features
Showing features for site, binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | monoatomic ion channel complex | |
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | ATP-activated inward rectifier potassium channel activity | |
Molecular Function | inward rectifier potassium channel activity | |
Molecular Function | peptide binding | |
Molecular Function | phosphatidylinositol-4,5-bisphosphate binding | |
Molecular Function | potassium ion binding | |
Biological Process | cellular response to magnesium ion | |
Biological Process | circulatory system development | |
Biological Process | gene expression | |
Biological Process | kidney development | |
Biological Process | negative regulation of apoptotic process | |
Biological Process | post-embryonic development | |
Biological Process | potassium ion import across plasma membrane | |
Biological Process | potassium ion transport | |
Biological Process | regulation of monoatomic ion transmembrane transport | |
Biological Process | renal sodium ion absorption | |
Biological Process | response to potassium ion | |
Biological Process | tissue homeostasis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-sensitive inward rectifier potassium channel 1
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionP35560
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Note: Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane.
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-77 | Cytoplasmic | ||||
Sequence: MGASERSVFRVLIRALTERMFKHLRRWFITHIFGRSRQRARLVSKEGRCNIEFGNVDAQSRFIFFVDIWTTVLDLKW | ||||||
Transmembrane | 78-102 | Helical; Name=M1 | ||||
Sequence: RYKMTVFITAFLGSWFLFGLLWYVV | ||||||
Topological domain | 103-127 | Extracellular | ||||
Sequence: AYVHKDLPEFYPPDNRTPCVENING | ||||||
Intramembrane | 128-139 | Helical; Pore-forming; Name=H5 | ||||
Sequence: MTSAFLFSLETQ | ||||||
Intramembrane | 140-146 | Pore-forming | ||||
Sequence: VTIGYGF | ||||||
Topological domain | 147-155 | Extracellular | ||||
Sequence: RFVTEQCAT | ||||||
Transmembrane | 156-177 | Helical; Name=M2 | ||||
Sequence: AIFLLIFQSILGVIINSFMCGA | ||||||
Topological domain | 178-391 | Cytoplasmic | ||||
Sequence: ILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM |
Keywords
- Cellular component
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for chain, modified residue, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000154919 | 1-391 | ATP-sensitive inward rectifier potassium channel 1 | |||
Sequence: MGASERSVFRVLIRALTERMFKHLRRWFITHIFGRSRQRARLVSKEGRCNIEFGNVDAQSRFIFFVDIWTTVLDLKWRYKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVENINGMTSAFLFSLETQVTIGYGFRFVTEQCATAIFLLIFQSILGVIINSFMCGAILAKISRPKKRAKTITFSKNAVISKRGGKLCLLIRVANLRKSLLIGSHIYGKLLKTTITPEGETIILDQTNINFVVDAGNENLFFISPLTIYHIIDHNSPFFHMAAETLSQQDFELVVFLDGTVESTSATCQVRTSYVPEEVLWGYRFVPIVSKTKEGKYRVDFHNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVLSEVDETDDTQM | ||||||
Modified residue | 44 | Phosphoserine; by SGK1 | ||||
Sequence: S | ||||||
Glycosylation | 117 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Post-translational modification
Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Mainly in kidney (renal cortex, medulla and papilla). In the brain, found in the cortex and the hippocampus.
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 141-146 | Selectivity filter | ||||
Sequence: TIGYGF |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 6 isoforms produced by Alternative splicing.
P35560-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameA
- Length391
- Mass (Da)44,963
- Last updated1994-06-01 v1
- Checksum896FD0FD128F85D0
P35560-2
- NameB
- SynonymsROMK2
- Differences from canonical
- 1-19: Missing
P35560-3
- NameC
- SynonymsROMK3
- Differences from canonical
- 1-12: MGASERSVFRVL → MVSELSIPSIPTGVAGLSK
P35560-4
- NameD
- Synonyms1.1, ROMK1.1
P35560-5
- NameE
- Synonyms3.1, ROMK3.1
P35560-6
- NameF
- Synonyms6.1, ROMK6.1
Computationally mapped potential isoform sequences
There are 5 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0G2K298 | A0A0G2K298_RAT | Kcnj1 | 218 | ||
A0A0G2JYW2 | A0A0G2JYW2_RAT | Kcnj1 | 391 | ||
A0A8I6AW52 | A0A8I6AW52_RAT | Kcnj1 | 372 | ||
A0A8I5ZJL6 | A0A8I5ZJL6_RAT | Kcnj1 | 398 | ||
A0A8I5ZLP0 | A0A8I5ZLP0_RAT | Kcnj1 | 398 |
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_002800 | 1-12 | in isoform C and isoform E | |||
Sequence: MGASERSVFRVL → MVSELSIPSIPTGVAGLSK | ||||||
Alternative sequence | VSP_002799 | 1-19 | in isoform B and isoform F | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_002801 | 79-124 | in isoform D, isoform E and isoform F | |||
Sequence: YKMTVFITAFLGSWFLFGLLWYVVAYVHKDLPEFYPPDNRTPCVEN → DSSDHRLRIQVCDRTVRHCHFPAYLPVYSWSDHQFLHVWCHFSQDL | ||||||
Alternative sequence | VSP_002802 | 125-391 | in isoform D, isoform E and isoform F | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X72341 EMBL· GenBank· DDBJ | CAA51068.1 EMBL· GenBank· DDBJ | mRNA | ||
L29403 EMBL· GenBank· DDBJ | AAA50378.1 EMBL· GenBank· DDBJ | mRNA | ||
S69385 EMBL· GenBank· DDBJ | AAB30553.1 EMBL· GenBank· DDBJ | mRNA | ||
AF081365 EMBL· GenBank· DDBJ | AAC34443.1 EMBL· GenBank· DDBJ | mRNA | ||
AF081366 EMBL· GenBank· DDBJ | AAC34444.1 EMBL· GenBank· DDBJ | mRNA | ||
AF081367 EMBL· GenBank· DDBJ | AAC34445.1 EMBL· GenBank· DDBJ | mRNA | ||
AF081368 EMBL· GenBank· DDBJ | AAC34446.1 EMBL· GenBank· DDBJ | mRNA |