P35513 · PAL2_TOBAC
- ProteinPhenylalanine ammonia-lyase
- GenePALA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids712 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score3/5
Function
function
This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.
Catalytic activity
- L-phenylalanine = (E)-cinnamate + NH4+
Pathway
Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 104 | Proton donor/acceptor | ||||
Sequence: Y | ||||||
Binding site | 256 | (E)-cinnamate (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 344 | (E)-cinnamate (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 350 | (E)-cinnamate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 380 | (E)-cinnamate (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 452 | (E)-cinnamate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 480 | (E)-cinnamate (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 483 | (E)-cinnamate (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ammonia-lyase activity | |
Molecular Function | phenylalanine ammonia-lyase activity | |
Biological Process | cinnamic acid biosynthetic process | |
Biological Process | L-phenylalanine catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhenylalanine ammonia-lyase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Nicotianoideae > Nicotianeae > Nicotiana
Accessions
- Primary accessionP35513
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, cross-link, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000215424 | 1-712 | Phenylalanine ammonia-lyase | |||
Sequence: MAGVAQNGHQEMDFCMKVDPLNWEMAADSLKGSHLDEVKKMVAEFRKPVVKLGGETLTVAQVAAIAAKDNVKTVKVELSEGARAGVKASSDWVMDSMGKGTDSYGVTTGFGATSHRRTKNGGALQKELIRFLNAGVFGNGTESCHTLPQSGTRAAMLVRINTLLQGYSGIRFEILEAITKLLNHNVTPCLPLRGTITASGDLVPLSYIAGLLTGRPNSKAVGPNGETLNAEEAFRVAGVNGGFFELQPKEGLALVNGTAVGSGLASMVLFDANVLAVFSEVLSAIFAEVMNGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSSYVKAAQKLHETDPLQKPKQDRYALRTSPQWLGPQIEVIRSATKMIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNARLALASIGKLMFGQFSELVNDYYNNGLPSNLTAGRNPSLDYGFKGSEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLDLISARKTAEAVDILKLMSSTYLVALCQAIDLRHLEENLRNAVKNTVSQVAKRTLTMGTNGELHPSRFCEKDLLRVVDREYVFAYADDACSANYPLMQKLRQVLVDHALQNGENEKNANSSIFQKILAFEDELKAVLPKEVESARAALESGNPAIANRIKECRSYPLYRFVRGELGAELLTGEKVRSPGEECDKVFTAMCNGQIIDSLLECLKEWNGAPLPIC | ||||||
Cross-link | 198↔200 | 5-imidazolinone (Ala-Gly) | ||||
Sequence: ASG | ||||||
Modified residue | 199 | 2,3-didehydroalanine (Ser) | ||||
Sequence: S |
Post-translational modification
Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly.
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length712
- Mass (Da)77,345
- Last updated1998-07-15 v2
- ChecksumE65F8A867FEA2C41
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D17467 EMBL· GenBank· DDBJ | BAA22963.1 EMBL· GenBank· DDBJ | mRNA | ||
AB008199 EMBL· GenBank· DDBJ | BAA22947.1 EMBL· GenBank· DDBJ | Genomic DNA |