P35193 · ATG19_YEAST
- ProteinAutophagy-related protein 19
- GeneATG19
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids415 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Cargo-receptor protein involved in the cytoplasm to vacuole transport (Cvt) and in autophagy. Recognizes cargo proteins, such as APE4, LAP3, LAP4 and AMS1 and delivers them to the pre-autophagosomal structure for eventual engulfment by the autophagosome and targeting to the vacuole. Involved in the organization of the preautophagosomal structure (PAS). ATG19 association with cargo protein is required to localize ATG11 to the PAS. Also involved in endoplasmic reticulum-specific autophagic process, in selective removal of ER-associated degradation (ERAD) substrates, and is essential for the survival of cells subjected to severe ER stress. Also plays a role in regulation of filamentous growth.
Miscellaneous
Present with 1250 molecules/cell in log phase SD medium.
Biotechnology
ATG19 is important for yeast autolysis which is the source of several molecules responsible for the quality of wines aged in contact with yeast cells.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | Cvt complex | |
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | phagophore assembly site | |
Cellular Component | phagophore assembly site membrane | |
Molecular Function | protein-macromolecule adaptor activity | |
Biological Process | autophagy | |
Biological Process | ERAD pathway | |
Biological Process | protein transport | |
Biological Process | protein-containing complex localization | |
Biological Process | vesicle organization |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAutophagy-related protein 19
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP35193
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Preautophagosomal structure membrane ; Peripheral membrane protein
Note: Also found in other perivacuolar punctate structures.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 412 | Impairs vacuolar transport of LAP4. | ||||
Sequence: W → A | ||||||
Mutagenesis | 415 | Impairs vacuolar transport of LAP4. | ||||
Sequence: L → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 11 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000064722 | 1-415 | Autophagy-related protein 19 | |||
Sequence: MNNSKTNQQMNTSMGYPLTVYDECNKFQLIVPTLDANIMLWCIGQLSLLNDSNGCKHLFWQPNDKSNVRILLNNYDYGHLFKYLQCQRKCSVYIGEGTLKKYNLTISTSFDNFLDLTPSEEKESLCREDAHEDPVSPKAGSEEEISPNSTSNVVVSRECLDNFMKQLLKLEESLNKLELEQKVTNKEPNHRISGTIDIPEDRSELVNFFTELKTVKQLEDVFQRYHDYERLSQECDSKTEIASDHSKKETKIEVEPPNERSLQITMNQRDNSLYFQLFNNTNSVLAGNCKLKFTDAGDKPTTQIIDMGPHEIGIKEYKEYRYFPYALDLEAGSTIEIENQYGEVIFLGKYGSSPMINLRPPSRLSAESLQASQEPFYSFQIDTLPELDDSSIISTSISLSYDGDDNEKALTWEEL | ||||||
Modified residue | 136 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 141 | Phosphoserine | ||||
Sequence: S | ||||||
Cross-link | 213 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K | ||||||
Cross-link | 216 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K | ||||||
Modified residue | 243 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Polyubiquitinated at Lys-213 and Lys-216. Deubiquitination by UBP3 is required for full activity of ATG19.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with the vacuolar aminopeptidase 1 (LAP4) precursor and mature forms. Also interacts with AMS1, APE4, ATG8 ATG11, and UBP3.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P35193 | APE1 P14904 | 4 | EBI-29291, EBI-2571 | |
BINARY | P35193 | ATG1 P53104 | 2 | EBI-29291, EBI-2657 | |
BINARY | P35193 | ATG11 Q12527 | 5 | EBI-29291, EBI-31977 | |
BINARY | P35193 | ATG8 P38182 | 5 | EBI-29291, EBI-2684 | |
BINARY | P35193 | HRR25 P29295 | 2 | EBI-29291, EBI-8536 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, coiled coil, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 21-28 | ATG11-binding | ||||
Sequence: YDECNKFQ | ||||||
Region | 126-150 | Disordered | ||||
Sequence: CREDAHEDPVSPKAGSEEEISPNST | ||||||
Coiled coil | 157-187 | |||||
Sequence: RECLDNFMKQLLKLEESLNKLELEQKVTNKE | ||||||
Region | 254-367 | AMS1-binding | ||||
Sequence: VEPPNERSLQITMNQRDNSLYFQLFNNTNSVLAGNCKLKFTDAGDKPTTQIIDMGPHEIGIKEYKEYRYFPYALDLEAGSTIEIENQYGEVIFLGKYGSSPMINLRPPSRLSAE | ||||||
Region | 406-415 | ATG8-binding | ||||
Sequence: NEKALTWEEL | ||||||
Motif | 412-415 | WXXL | ||||
Sequence: WEEL |
Domain
The coiled coil domain is required for the interaction with LAP4.
The C-terminal WXXL motif is crucial for ATG8-binding and Cvt pathway.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length415
- Mass (Da)47,599
- Last updated1994-02-01 v1
- ChecksumB49DA1DF83A04070
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 412 | in Ref. 5; AAT93193 | ||||
Sequence: W → R |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X75449 EMBL· GenBank· DDBJ | CAA53201.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X83121 EMBL· GenBank· DDBJ | CAA58199.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z74824 EMBL· GenBank· DDBJ | CAA99094.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY693174 EMBL· GenBank· DDBJ | AAT93193.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006948 EMBL· GenBank· DDBJ | DAA10702.1 EMBL· GenBank· DDBJ | Genomic DNA |