P34896 · GLYC_HUMAN
- ProteinSerine hydroxymethyltransferase, cytosolic
- GeneSHMT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids483 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Miscellaneous
In eukaryotes there are two forms of the enzymes: a cytosolic one and a mitochondrial one.
Catalytic activity
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
Cofactor
Activity regulation
Inhibited by tetrahydrofolate concentrations above 40 uM.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.18 mM | L-serine | |||||
5.2 μM | tetrahydrofolate |
Pathway
One-carbon metabolism; tetrahydrofolate interconversion.
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameSerine hydroxymethyltransferase, cytosolic
- EC number
- Short namesSHMT
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionP34896
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_059795 | 340 | in dbSNP:rs7215148 | |||
Sequence: E → Q | ||||||
Natural variant | VAR_022010 | 474 | in dbSNP:rs1979277 | |||
Sequence: L → F |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 976 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue (large scale data), modified residue.
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000113504 | 1-483 | UniProt | Serine hydroxymethyltransferase, cytosolic | |||
Sequence: MTMPVNGAHKDADLWSSHDKMLAQPLKDSDVEVYNIIKKESNRQRVGLELIASENFASRAVLEALGSCLNNKYSEGYPGQRYYGGTEFIDELETLCQKRALQAYKLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGYINYDQLEENARLFHPKLIIAGTSCYSRNLEYARLRKIADENGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLRGCRAGMIFYRKGVKSVDPKTGKEILYNLESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTLEFKVYQHQVVANCRALSEALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQKVAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAAVQALREEVESFASLFPLPGLPDF | |||||||
Modified residue (large scale data) | 34 | PRIDE | Phosphotyrosine | ||||
Sequence: Y | |||||||
Modified residue (large scale data) | 77 | PRIDE | Phosphotyrosine | ||||
Sequence: Y | |||||||
Modified residue | 257 | UniProt | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K | |||||||
Modified residue | 271 | UniProt | In isoform P34896-2; N6-acetyllysine | ||||
Sequence: K |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Homotetramer (PubMed:24698160, PubMed:25619277, PubMed:9753690).
Identified in complex with ABRAXAS2 and the other subunits of the BRISC complex, at least composed of ABRAXAS2, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1 (PubMed:24075985).
Identified in complex with ABRAXAS2 and the other subunits of the BRISC complex, at least composed of ABRAXAS2, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1 (PubMed:24075985).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P34896 | DDX6 P26196 | 3 | EBI-715117, EBI-351257 | |
BINARY | P34896 | GORASP2 Q9H8Y8 | 3 | EBI-715117, EBI-739467 | |
BINARY | P34896 | IDH3A P50213 | 6 | EBI-715117, EBI-355999 | |
BINARY | P34896 | MAPK9 P45984 | 7 | EBI-715117, EBI-713568 | |
BINARY | P34896 | MDFI Q99750 | 3 | EBI-715117, EBI-724076 | |
BINARY | P34896 | SHMT1 P34896 | 4 | EBI-715117, EBI-715117 | |
BINARY | P34896 | SULT1A3 P0DMM9 | 3 | EBI-715117, EBI-10196922 |
Protein-protein interaction databases
Chemistry
Miscellaneous
Structure
Sequence & Isoforms
- Sequence statusComplete
This entry describes 4 isoforms produced by Alternative splicing.
P34896-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length483
- Mass (Da)53,083
- Last updated1994-02-01 v1
- Checksum6CDD6CA06D017C19
P34896-2
- Name2
- Differences from canonical
- 274-312: Missing
P34896-3
- Name3
- Differences from canonical
- 273-352: Missing
P34896-4
- Name4
- Differences from canonical
- 1-138: Missing
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L11931 EMBL· GenBank· DDBJ | AAA63257.1 EMBL· GenBank· DDBJ | mRNA | ||
L23928 EMBL· GenBank· DDBJ | AAA36020.1 EMBL· GenBank· DDBJ | mRNA | ||
L23928 EMBL· GenBank· DDBJ | AAA36019.1 EMBL· GenBank· DDBJ | mRNA | ||
L23928 EMBL· GenBank· DDBJ | AAA36018.1 EMBL· GenBank· DDBJ | mRNA | ||
Y14485 EMBL· GenBank· DDBJ | CAB54838.1 EMBL· GenBank· DDBJ | mRNA | ||
Y14486 EMBL· GenBank· DDBJ | CAB54839.1 EMBL· GenBank· DDBJ | mRNA | ||
Y14487 EMBL· GenBank· DDBJ | CAB54840.1 EMBL· GenBank· DDBJ | mRNA | ||
AK298415 EMBL· GenBank· DDBJ | BAG60641.1 EMBL· GenBank· DDBJ | mRNA | ||
AC127537 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL353997 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH471196 EMBL· GenBank· DDBJ | EAW55637.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471196 EMBL· GenBank· DDBJ | EAW55640.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471196 EMBL· GenBank· DDBJ | EAW55641.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC007979 EMBL· GenBank· DDBJ | AAH07979.1 EMBL· GenBank· DDBJ | mRNA | ||
BC022874 EMBL· GenBank· DDBJ | AAH22874.1 EMBL· GenBank· DDBJ | mRNA | ||
BC038598 EMBL· GenBank· DDBJ | AAH38598.1 EMBL· GenBank· DDBJ | mRNA |