P34647 · MCM6_CAEEL

  • Protein
    DNA replication licensing factor mcm-6
  • Gene
    mcm-6
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Acts as a component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity).

Miscellaneous

Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site397-404ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular ComponentMCM complex
Cellular Componentnucleus
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionDNA helicase activity
Molecular Functionsingle-stranded DNA binding
Biological ProcessDNA replication initiation
Biological ProcessDNA unwinding involved in DNA replication
Biological Processdouble-strand break repair via break-induced replication
Biological Processmitotic DNA replication
Biological Processpremeiotic DNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA replication licensing factor mcm-6
  • EC number

Gene names

    • Name
      mcm-6
    • ORF names
      ZK632.1

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    P34647
  • Secondary accessions
    • Q5FC84

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001941161-810DNA replication licensing factor mcm-6

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

Component of the mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (By simililarity).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P34647cya-1 P346384EBI-2006759, EBI-2421605

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain346-554MCM
Motif529-532Arginine finger

Sequence similarities

Belongs to the MCM family.

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

P34647-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    810
  • Mass (Da)
    91,130
  • Last updated
    1994-02-01 v1
  • Checksum
    214B65CFCE76EEB1
MDNIIGGQAQQVEDTDGTRVQNEFSKFLKSFKDQKNEFIYKSAMKELVQPEKNTIFINMQHLYKFSNNLATTIELQYYRVYPFMCEALHLATLDACDESERQQMFKKQLYVSLFNLDAKTKVRELSADKVGGLVRIAGQIVRTHPVHPELSRACFVCEDCGVTTRDVQQQFRYTQPTKCANPQCMNRTRFSLDVNSSTFVDFQKIRIQETQAELPRGSIPRTVDVIVRGEMVETVQPGDKCDIVGTLIVIPDIAQLSTPGLRAETSNQNRGRATDKSEGITGLKALGVRDLTYKMAFLACHIQQTESLVGGDASGAVEETDYLDLWSKMSTEDRATLKKMSDDKKIEKNIVDSLFPNIYGNHEVKLGVLLMLLGGVAKKSRDEGTSLRGDINVCLVGDPSTAKSQVLKAVEEFSPRAIYTSGKASSAAGLTAAVVKDEESFEFVIEAGALMLADNGVCCIDEFDKMDLKDQVAIHEAMEQQTISITKAGVKATLNARASILAAANPVNGRYDRSRPLKYNVQMSAPIMSRFDLFFVLVDECNEVTDYAIARRILDNHRAISEHTERDSVYKIDDIKKYIAFARCFKPKISDKAAETLVREYKKLRMSDSNNAATSSWRITVRQLESLVRLSEALARLHCGKEVLVEHVEKAAELLNKSIVRVEQPDIALDDDDFDNNIMVVEADKENQRGDDSMDHDGEKENAPKIDIAKLKISFKEYKQLSDVLVLHMRSDEDNQGEDEYDGVKQSALVEWYLSTIEADLETEEDFNVQKTICERVIHRLIHQDHVLLEVEQGEDPTLCVHPNYVIADE

P34647-2

  • Name
    b
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0178961-294in isoform b

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z22181
EMBL· GenBank· DDBJ
CAA80191.1
EMBL· GenBank· DDBJ
Genomic DNA
Z29095
EMBL· GenBank· DDBJ
CAA80191.1
EMBL· GenBank· DDBJ
Genomic DNA
Z22181
EMBL· GenBank· DDBJ
CAI46590.1
EMBL· GenBank· DDBJ
Genomic DNA
AF326940
EMBL· GenBank· DDBJ
AAG49390.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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