P34647 · MCM6_CAEEL
- ProteinDNA replication licensing factor mcm-6
- Genemcm-6
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids810 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Acts as a component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity).
Miscellaneous
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphateThis reaction proceeds in the forward direction.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | MCM complex | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA helicase activity | |
Molecular Function | single-stranded DNA binding | |
Biological Process | DNA replication initiation | |
Biological Process | DNA unwinding involved in DNA replication | |
Biological Process | double-strand break repair via break-induced replication | |
Biological Process | mitotic DNA replication | |
Biological Process | premeiotic DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA replication licensing factor mcm-6
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionP34647
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000194116 | 1-810 | DNA replication licensing factor mcm-6 | |||
Sequence: MDNIIGGQAQQVEDTDGTRVQNEFSKFLKSFKDQKNEFIYKSAMKELVQPEKNTIFINMQHLYKFSNNLATTIELQYYRVYPFMCEALHLATLDACDESERQQMFKKQLYVSLFNLDAKTKVRELSADKVGGLVRIAGQIVRTHPVHPELSRACFVCEDCGVTTRDVQQQFRYTQPTKCANPQCMNRTRFSLDVNSSTFVDFQKIRIQETQAELPRGSIPRTVDVIVRGEMVETVQPGDKCDIVGTLIVIPDIAQLSTPGLRAETSNQNRGRATDKSEGITGLKALGVRDLTYKMAFLACHIQQTESLVGGDASGAVEETDYLDLWSKMSTEDRATLKKMSDDKKIEKNIVDSLFPNIYGNHEVKLGVLLMLLGGVAKKSRDEGTSLRGDINVCLVGDPSTAKSQVLKAVEEFSPRAIYTSGKASSAAGLTAAVVKDEESFEFVIEAGALMLADNGVCCIDEFDKMDLKDQVAIHEAMEQQTISITKAGVKATLNARASILAAANPVNGRYDRSRPLKYNVQMSAPIMSRFDLFFVLVDECNEVTDYAIARRILDNHRAISEHTERDSVYKIDDIKKYIAFARCFKPKISDKAAETLVREYKKLRMSDSNNAATSSWRITVRQLESLVRLSEALARLHCGKEVLVEHVEKAAELLNKSIVRVEQPDIALDDDDFDNNIMVVEADKENQRGDDSMDHDGEKENAPKIDIAKLKISFKEYKQLSDVLVLHMRSDEDNQGEDEYDGVKQSALVEWYLSTIEADLETEEDFNVQKTICERVIHRLIHQDHVLLEVEQGEDPTLCVHPNYVIADE |
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Component of the mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (By simililarity).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P34647 | cya-1 P34638 | 4 | EBI-2006759, EBI-2421605 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 346-554 | MCM | ||||
Sequence: IEKNIVDSLFPNIYGNHEVKLGVLLMLLGGVAKKSRDEGTSLRGDINVCLVGDPSTAKSQVLKAVEEFSPRAIYTSGKASSAAGLTAAVVKDEESFEFVIEAGALMLADNGVCCIDEFDKMDLKDQVAIHEAMEQQTISITKAGVKATLNARASILAAANPVNGRYDRSRPLKYNVQMSAPIMSRFDLFFVLVDECNEVTDYAIARRIL | ||||||
Motif | 529-532 | Arginine finger | ||||
Sequence: SRFD |
Sequence similarities
Belongs to the MCM family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
P34647-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length810
- Mass (Da)91,130
- Last updated1994-02-01 v1
- Checksum214B65CFCE76EEB1
P34647-2
- Nameb
- Differences from canonical
- 1-294: Missing
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_017896 | 1-294 | in isoform b | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z22181 EMBL· GenBank· DDBJ | CAA80191.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z29095 EMBL· GenBank· DDBJ | CAA80191.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z22181 EMBL· GenBank· DDBJ | CAI46590.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF326940 EMBL· GenBank· DDBJ | AAG49390.1 EMBL· GenBank· DDBJ | mRNA |