P34216 · EDE1_YEAST
- ProteinEH domain-containing and endocytosis protein 1
- GeneEDE1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1381 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Functions at the internalization step of the clathrin-mediated endocytosis (CME) as an early-acting scaffold protein. Requires clathrin adapter proteins, ENT1/2 and YAP1801/2, for normal spatiotemporal dynamics and viability. Binds to biological membranes in a ubiquitin-dependent manner.
Miscellaneous
Present with 1380 molecules/cell in log phase SD medium.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 180 | Ca2+ (UniProtKB | ChEBI) | |||
Binding site | 182 | Ca2+ (UniProtKB | ChEBI) | |||
Binding site | 184 | Ca2+ (UniProtKB | ChEBI) | |||
Binding site | 191 | Ca2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | actin cortical patch | |
Cellular Component | cellular bud neck | |
Cellular Component | cellular bud tip | |
Cellular Component | cytoplasm | |
Cellular Component | mating projection tip | |
Cellular Component | plasma membrane | |
Molecular Function | calcium ion binding | |
Molecular Function | ubiquitin binding | |
Biological Process | actin cortical patch organization | |
Biological Process | endocytosis | |
Biological Process | endoplasmic reticulum unfolded protein response | |
Biological Process | endosomal transport | |
Biological Process | positive regulation of cytokinesis | |
Biological Process | regulation of protein localization |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEH domain-containing and endocytosis protein 1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP34216
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localized to actin cortical patches concentrated in the developing bud tip in cells with small buds and at the mother-daughter neck in cells undergoing cytokinesis. Localization can be maintained in the absence of polymerized actin filaments.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Random budding pattern and morphological defects. Defects in fluid-phase endocytosis and defective internalization of the pheromone alpha-factor and uracil permease. Deletion has only a small impact on actin cytoskeleton organization. Deletion shows synthetic growth defects with thermosensitive mutants of PAN1, END3 and RSP5.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 56 | Abnormal spatiotemporal behavior. | |||
Mutagenesis | 176 | Abnormal spatiotemporal behavior. | |||
Mutagenesis | 319 | Abnormal spatiotemporal behavior. | |||
Mutagenesis | 1352 | Reduced ubiquitin-binding. | |||
Mutagenesis | 1370 | Reduced ubiquitin-binding. | |||
Mutagenesis | 1374 | Enhanced ubiquitin-binding. | |||
Mutagenesis | 1378 | Reduced ubiquitin-binding. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 22 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue, cross-link.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000202457 | 1-1381 | EH domain-containing and endocytosis protein 1 | ||
Modified residue | 238 | Phosphothreonine | |||
Modified residue | 241 | Phosphoserine | |||
Modified residue | 244 | Phosphoserine | |||
Modified residue | 245 | Phosphothreonine | |||
Modified residue | 248 | Phosphoserine | |||
Modified residue | 249 | Phosphoserine | |||
Modified residue | 251 | Phosphothreonine | |||
Modified residue | 265 | Phosphoserine | |||
Modified residue | 419 | Phosphoserine | |||
Modified residue | 450 | Phosphothreonine | |||
Modified residue | 477 | Phosphothreonine | |||
Modified residue | 487 | Phosphothreonine | |||
Modified residue | 495 | Phosphoserine | |||
Cross-link | 674 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | |||
Modified residue | 848 | Phosphoserine | |||
Modified residue | 931 | Phosphoserine | |||
Modified residue | 950 | Phosphoserine | |||
Modified residue | 964 | Phosphoserine | |||
Modified residue | 1008 | Phosphoserine | |||
Modified residue | 1012 | Phosphoserine | |||
Modified residue | 1020 | Phosphoserine | |||
Modified residue | 1046 | Phosphothreonine | |||
Modified residue | 1069 | Phosphoserine | |||
Modified residue | 1087 | Phosphoserine | |||
Modified residue | 1093 | Phosphoserine | |||
Modified residue | 1095 | Phosphoserine | |||
Modified residue | 1096 | Phosphoserine | |||
Modified residue | 1100 | Phosphoserine | |||
Modified residue | 1111 | Phosphothreonine | |||
Modified residue | 1181 | Phosphoserine | |||
Modified residue | 1187 | Phosphoserine | |||
Modified residue | 1307 | Phosphothreonine | |||
Cross-link | 1329 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | |||
Modified residue | 1343 | Phosphoserine | |||
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts (via UBA domain) with monoubiquitin and ENT1 (via asparagine-proline-phenylalanine tripeptide motif called NPF). Interacts with PAL1 and SYP1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | P34216 | SYP1 P25623 | 7 | EBI-21243, EBI-21900 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region, coiled coil.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 14-113 | EH 1 | |||
Domain | 47-82 | EF-hand 1 | |||
Domain | 135-227 | EH 2 | |||
Domain | 167-202 | EF-hand 2 | |||
Domain | 276-311 | EF-hand 3 | |||
Domain | 277-366 | EH 3 | |||
Compositional bias | 389-497 | Polar residues | |||
Region | 389-535 | Disordered | |||
Coiled coil | 593-882 | ||||
Region | 898-919 | Disordered | |||
Region | 933-1202 | Disordered | |||
Compositional bias | 935-954 | Basic and acidic residues | |||
Compositional bias | 957-992 | Polar residues | |||
Compositional bias | 1003-1019 | Polar residues | |||
Compositional bias | 1025-1039 | Basic and acidic residues | |||
Compositional bias | 1062-1107 | Polar residues | |||
Compositional bias | 1145-1167 | Polar residues | |||
Compositional bias | 1174-1194 | Polar residues | |||
Region | 1214-1285 | Disordered | |||
Region | 1217-1381 | Able to bind biological membranes | |||
Compositional bias | 1253-1284 | Polar residues | |||
Region | 1298-1322 | Disordered | |||
Domain | 1338-1380 | UBA | |||
Sequence similarities
Belongs to the VDP/USO1/EDE1 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,381
- Mass (Da)150,783
- Last updated1994-10-01 v2
- Checksum626FD261DCBA7D99
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 389-497 | Polar residues | |||
Compositional bias | 935-954 | Basic and acidic residues | |||
Compositional bias | 957-992 | Polar residues | |||
Compositional bias | 1003-1019 | Polar residues | |||
Compositional bias | 1025-1039 | Basic and acidic residues | |||
Compositional bias | 1062-1107 | Polar residues | |||
Compositional bias | 1145-1167 | Polar residues | |||
Compositional bias | 1174-1194 | Polar residues | |||
Compositional bias | 1253-1284 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z35808 EMBL· GenBank· DDBJ | CAA84867.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X78214 EMBL· GenBank· DDBJ | CAA55048.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z23261 EMBL· GenBank· DDBJ | CAA80797.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006936 EMBL· GenBank· DDBJ | DAA07071.1 EMBL· GenBank· DDBJ | Genomic DNA |