P33681 · CD80_HUMAN
- ProteinT-lymphocyte activation antigen CD80
- GeneCD80
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids288 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (PubMed:38467718).
Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed:12196291).
In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (PubMed:10438913).
In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (PubMed:12121659).
Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (PubMed:36727298).
Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (By similarity).
Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells together with the CD28 receptor which is constitutively expressed on the cell surface of T-cells (PubMed:12196291).
In turn, activates different signaling pathways such as NF-kappa-B or MAPK leading to the production of different cytokines (PubMed:10438913).
In addition, CD28/CD80 costimulatory signal stimulates glucose metabolism and ATP synthesis of T-cells by activating the PI3K/Akt signaling pathway (PubMed:12121659).
Acts also as a regulator of PDL1/PDCD1 interactions to limit excess engagement of PDL1 and its inhibitory role in immune responses (PubMed:36727298).
Expressed on B-cells, plays a critical role in regulating interactions between B-cells and T-cells in both early and late germinal center responses, which are crucial for the generation of effective humoral immune responses (By similarity).
(Microbial infection) Acts as a receptor for adenovirus subgroup B.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameT-lymphocyte activation antigen CD80
- Alternative names
- CD Antigen Name
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionP33681
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Single-pass type I membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 35-242 | Extracellular | ||||
Sequence: VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN | ||||||
Transmembrane | 243-263 | Helical | ||||
Sequence: LLPSWAITLISVNGIFVICCL | ||||||
Topological domain | 264-288 | Cytoplasmic | ||||
Sequence: TYCFAPRCRERRRNERLRRESVRPV |
Keywords
- Cellular component
Disease & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 261 | Loss of costimulatory function upon T-cell activation; when associated with S-262, S-266 and S-271. | ||||
Sequence: C → S | ||||||
Mutagenesis | 262 | Loss of costimulatory function upon T-cell activation; when associated with S-261, S-266 and S-271. | ||||
Sequence: C → S | ||||||
Mutagenesis | 266 | Loss of costimulatory function upon T-cell activation; when associated with S-261, S-262 and S-271. | ||||
Sequence: C → S | ||||||
Mutagenesis | 271 | Loss of costimulatory function upon T-cell activation; when associated with S-261, S-262 and S-266. | ||||
Sequence: C → S |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 365 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for signal, chain, disulfide bond, glycosylation, lipidation, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-34 | |||||
Sequence: MGHTRRQGTSPSKCPYLNFFQLLVLAGLSHFCSG | ||||||
Chain | PRO_0000014547 | 35-288 | T-lymphocyte activation antigen CD80 | |||
Sequence: VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDNLLPSWAITLISVNGIFVICCLTYCFAPRCRERRRNERLRRESVRPV | ||||||
Disulfide bond | 50↔116 | |||||
Sequence: CGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYEC | ||||||
Glycosylation | 53 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 89 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 98 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 162↔216 | |||||
Sequence: CSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMC | ||||||
Glycosylation | 186 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 207 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 211 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 226 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 232 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Lipidation | 261 | S-palmitoyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 262 | S-palmitoyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 266 | S-palmitoyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 271 | S-palmitoyl cysteine | ||||
Sequence: C | ||||||
Modified residue | 284 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Palmitoylated by ZDHHC20; palmitoylation protects CD80 from ubiquitin-mediated degradation, regulating the protein stability, and ensures its accurate plasma membrane localization.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells.
Gene expression databases
Organism-specific databases
Interaction
Subunit
Homodimer. Interacts with CTLA4; this interaction inhibits T-cell activation (PubMed:10583602, PubMed:11279502).
Interacts with PDL1/CD274; this interaction blocks PDL1/PDCD1 binding and thus PDL1/CD274 inhibitory function (PubMed:36727298).
Interacts with CD28 (PubMed:12196291).
Interacts with PDL1/CD274; this interaction blocks PDL1/PDCD1 binding and thus PDL1/CD274 inhibitory function (PubMed:36727298).
Interacts with CD28 (PubMed:12196291).
(Microbial infection) Interacts with adenovirus subgroup B fiber proteins.
(Microbial infection) Interacts with Orthopoxvirus OPG038/M2 protein, inhibiting the interaction with CTLA4/CD152.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P33681 | CD274 Q9NZQ7 | 11 | EBI-1031024, EBI-4314282 | |
BINARY | P33681 | CTLA4 P16410 | 7 | EBI-1031024, EBI-1030991 | |
BINARY | P33681 | NGFR P08138 | 3 | EBI-1031024, EBI-1387782 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 35-135 | Ig-like V-type | ||||
Sequence: VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVT | ||||||
Domain | 145-230 | Ig-like C2-type | ||||
Sequence: PSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFN |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 3 isoforms produced by Alternative splicing.
P33681-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length288
- Mass (Da)33,048
- Last updated1994-02-01 v1
- ChecksumBA453EE34528B1F4
P33681-2
- Name2
- Synonymss1CD80
- NoteSoluble isoform. Expressed in unstimulated B-cells and monocytes, but not T-cells.
- Differences from canonical
- 234-266: TKQEHFPDNLLPSWAITLISVNGIFVICCLTYC → S
P33681-3
- Name3
- Synonymss2CD80
- NoteSoluble isoform. Expressed in T-cells activated by ConA, non-activated monocytes and monocytes activated with IFN-c.
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M27533 EMBL· GenBank· DDBJ | AAA36045.1 EMBL· GenBank· DDBJ | mRNA | ||
M83077 EMBL· GenBank· DDBJ | AAA58390.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M83072 EMBL· GenBank· DDBJ | AAA58390.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M83073 EMBL· GenBank· DDBJ | AAA58390.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M83074 EMBL· GenBank· DDBJ | AAA58390.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY197777 EMBL· GenBank· DDBJ | AAO39208.1 EMBL· GenBank· DDBJ | mRNA | ||
AY197778 EMBL· GenBank· DDBJ | AAO39209.1 EMBL· GenBank· DDBJ | mRNA | ||
AC073352 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC042665 EMBL· GenBank· DDBJ | AAH42665.1 EMBL· GenBank· DDBJ | mRNA |