P33085 · SHAKB_DROME
- ProteinInnexin shaking-B
- GeneshakB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids372 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Structural component of the gap junctions at electrical synapses in distal and mid-depth levels in the lamina. Isoform Lethal forms voltage sensitive intercellular channels through homotypic interactions.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | gap junction | |
Cellular Component | plasma membrane | |
Molecular Function | gap junction channel activity | |
Molecular Function | photoreceptor activity | |
Biological Process | cell communication by electrical coupling | |
Biological Process | gap junction assembly | |
Biological Process | intercellular transport | |
Biological Process | jump response | |
Biological Process | monoatomic ion transmembrane transport | |
Biological Process | phototransduction | |
Biological Process | regulation of membrane depolarization | |
Biological Process | response to light stimulus | |
Biological Process | transmembrane transport |
Keywords
- Molecular function
- Biological process
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameInnexin shaking-B
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionP33085
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-21 | Cytoplasmic | ||||
Sequence: MLDIFRGLKNLVKVSHVKTDS | ||||||
Transmembrane | 22-42 | Helical | ||||
Sequence: IVFRLHYSITVMILMSFSLII | ||||||
Topological domain | 43-110 | Extracellular | ||||
Sequence: TTRQYVGNPIDCVHTKDIPEDVLNTYCWIQSTYTLKSLFLKKQGVSVPYPGIGNSDGDPADKKHYKYY | ||||||
Transmembrane | 111-131 | Helical | ||||
Sequence: QWVCFCLFFQAILFYTPRWLW | ||||||
Topological domain | 132-182 | Cytoplasmic | ||||
Sequence: KSWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYHNWWAYRYY | ||||||
Transmembrane | 183-203 | Helical | ||||
Sequence: VCELLALINVIGQMFLMNRFF | ||||||
Topological domain | 204-267 | Extracellular | ||||
Sequence: DGEFITFGLKVIDYMETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEK | ||||||
Transmembrane | 268-288 | Helical | ||||
Sequence: IYIFLWFWFILLTFLTLLTLI | ||||||
Topological domain | 289-372 | Cytoplasmic | ||||
Sequence: YRVVIIFSPRMRVYLFRMRFRLVRRDAIEIIVRRSKMGDWFLLYLLGENIDTVIFRDVVQDLANRLGHNQHHRVPGLKGEIQDA |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Flies fail to jump in response to a light-off stimulus. Neural-specific mutants exhibit modified giant fiber system and gustatory response.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000208500 | 1-372 | Innexin shaking-B | |||
Sequence: MLDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILMSFSLIITTRQYVGNPIDCVHTKDIPEDVLNTYCWIQSTYTLKSLFLKKQGVSVPYPGIGNSDGDPADKKHYKYYQWVCFCLFFQAILFYTPRWLWKSWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYHNWWAYRYYVCELLALINVIGQMFLMNRFFDGEFITFGLKVIDYMETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFWFILLTFLTLLTLIYRVVIIFSPRMRVYLFRMRFRLVRRDAIEIIVRRSKMGDWFLLYLLGENIDTVIFRDVVQDLANRLGHNQHHRVPGLKGEIQDA |
Proteomic databases
Expression
Tissue specificity
Isoform Neural is expressed in synapses of giant fibers (GF), in a large thoracic cell in location of postsynaptic target and optic lobe lamina and medulla. Isoform Lethal is expressed in embryonic mesodermal derivatives. During metamorphosis, both isoforms are dynamically expressed in pupal nervous system.
Gene expression databases
Structure
Sequence & Isoforms
- Sequence statusComplete
This entry describes 6 isoforms produced by Alternative splicing. Additional isoforms seem to exist.
P33085-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameLethal
- SynonymsA
- Length372
- Mass (Da)44,355
- Last updated2000-12-01 v3
- Checksum3DD1049D8F1D4481
P33085-2
- NameNeural
- SynonymsC
P33085-3
- NameB
P33085-4
- NameD
- Differences from canonical
- 2-116: LDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILMSFSLIITTRQYVGNPIDCVHTKDIPEDVLNTYCWIQSTYTLKSLFLKKQGVSVPYPGIGNSDGDPADKKHYKYYQWVCFC → YKPDTLTRRGSLRSLRSAPLLSTVLESTLSLTRIHPIASLELPGLDYAVHSQSAFGAYGLAHPRDLATCTSLRSGLAAITAASASAAGGVSQSQSALLGRYGPNASIRHGERKIVQPKRVLSRKLKPHLVADTVKQYISRAQRTTKKGSQEQQNMEFLRGVYAFMQVSRSSVSHVKIDSPVFRLHTNATVILLITFSIAVTTRQYVGNPIDCVHTRDIPEDVLNTYCWIHSTYTVVDAFMKKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFT
P33085-6
- NameE
- Differences from canonical
- 2-119: LDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILMSFSLIITTRQYVGNPIDCVHTKDIPEDVLNTYCWIQSTYTLKSLFLKKQGVSVPYPGIGNSDGDPADKKHYKYYQWVCFCLFF → MGRHCHVPKQTHHIQFAQLAQSPAVCGLSESAANLRYKLLLICHRVGIKIPRQKKIPTTYNE
P33085-5
- NameF
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
M9NGR8 | M9NGR8_DROME | shakB | 377 |
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_036914 | 2-36 | in isoform F | |||
Sequence: LDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILM → KKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFT | ||||||
Alternative sequence | VSP_002678 | 2-58 | in isoform Neural | |||
Sequence: LDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILMSFSLIITTRQYVGNPIDCVHTK → VSHVKIDSPVFRLHTNATVILLITFSIAVTTRQYVGNPIDCVHTR | ||||||
Alternative sequence | VSP_036913 | 2-116 | in isoform D | |||
Sequence: LDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILMSFSLIITTRQYVGNPIDCVHTKDIPEDVLNTYCWIQSTYTLKSLFLKKQGVSVPYPGIGNSDGDPADKKHYKYYQWVCFC → YKPDTLTRRGSLRSLRSAPLLSTVLESTLSLTRIHPIASLELPGLDYAVHSQSAFGAYGLAHPRDLATCTSLRSGLAAITAASASAAGGVSQSQSALLGRYGPNASIRHGERKIVQPKRVLSRKLKPHLVADTVKQYISRAQRTTKKGSQEQQNMEFLRGVYAFMQVSRSSVSHVKIDSPVFRLHTNATVILLITFSIAVTTRQYVGNPIDCVHTRDIPEDVLNTYCWIHSTYTVVDAFMKKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFT | ||||||
Alternative sequence | VSP_037474 | 2-119 | in isoform E | |||
Sequence: LDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILMSFSLIITTRQYVGNPIDCVHTKDIPEDVLNTYCWIQSTYTLKSLFLKKQGVSVPYPGIGNSDGDPADKKHYKYYQWVCFCLFF → MGRHCHVPKQTHHIQFAQLAQSPAVCGLSESAANLRYKLLLICHRVGIKIPRQKKIPTTYNE | ||||||
Sequence conflict | 3 | In isoform P33085-4; in Ref. 7; ABE73233 | ||||
Sequence: K → E | ||||||
Alternative sequence | VSP_036915 | 37-116 | in isoform F | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_002679 | 72-116 | in isoform Neural | |||
Sequence: QSTYTLKSLFLKKQGVSVPYPGIGNSDGDPADKKHYKYYQWVCFC → HSTYTVVDAFMKKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFT | ||||||
Alternative sequence | VSP_012897 | 121 | in isoform B | |||
Sequence: A → P | ||||||
Alternative sequence | VSP_012898 | 123-372 | in isoform B | |||
Sequence: Missing | ||||||
Sequence conflict | 232 | in Ref. 2; AAA28745 and 7; ABE73233 | ||||
Sequence: I → M | ||||||
Sequence conflict | 247-249 | in Ref. 4; AAC46584 | ||||
Sequence: SGE → GEV |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M98872 EMBL· GenBank· DDBJ | AAA89079.1 EMBL· GenBank· DDBJ | mRNA | ||
X65103 EMBL· GenBank· DDBJ | CAA46228.1 EMBL· GenBank· DDBJ | mRNA | ||
L13306 EMBL· GenBank· DDBJ | AAA28745.1 EMBL· GenBank· DDBJ | mRNA | ||
S78495 EMBL· GenBank· DDBJ | AAB34769.1 EMBL· GenBank· DDBJ | mRNA | ||
U17330 EMBL· GenBank· DDBJ | AAC46584.1 EMBL· GenBank· DDBJ | mRNA | ||
AE014298 EMBL· GenBank· DDBJ | AAF50880.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014298 EMBL· GenBank· DDBJ | AAF50882.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014298 EMBL· GenBank· DDBJ | AAF50883.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014298 EMBL· GenBank· DDBJ | ABW09456.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014298 EMBL· GenBank· DDBJ | ACL82952.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT025062 EMBL· GenBank· DDBJ | ABE73233.1 EMBL· GenBank· DDBJ | mRNA |