P32962 · NRL2_ARATH
- ProteinNitrilase 2
- GeneNIT2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids339 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Can convert indole-3-acetonitrile to the plant hormone indole-3-acetic acid.
Catalytic activity
- a nitrile + 2 H2O = a carboxylate + NH4+
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 58 | Proton acceptor | ||||
Sequence: E | ||||||
Active site | 145 | Proton donor | ||||
Sequence: K | ||||||
Active site | 179 | Nucleophile | ||||
Sequence: C |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | plasma membrane | |
Molecular Function | indole-3-acetonitrile nitrilase activity | |
Molecular Function | indole-3-acetonitrile nitrile hydratase activity | |
Molecular Function | mRNA binding | |
Molecular Function | nitrilase activity | |
Biological Process | indoleacetic acid biosynthetic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNitrilase 2
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionP32962
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Note: Tightly associated with the plasma membrane.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylserine | ||||
Sequence: S | ||||||
Chain | PRO_0000204037 | 2-339 | Nitrilase 2 | |||
Sequence: STSENTPFNGVASSTIVRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVGVHNEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFFSPQGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDKEPDSIVSQGGSVIISPLGQVLAGPNFESEGLITADLDLGDVARAKLYFDSVGHYSRPDVLHLTVNEHPKKPVTFISKVEKAEDDSNK |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
By indole-3-acetonitrile, abscisic acid (ABA), salicylic acid (SA), sodium nitroprusside (SNP), salt stress and dehydration stress.
Developmental stage
Barely detectable in young rosettes, but is strongly expressed during bolting, flowering, and especially fruit development.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 18-290 | CN hydrolase | ||||
Sequence: VRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVGVHNEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFFSPQGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDKEPDSIVSQGGSVIISPLGQVLAGPNFESEGLITADLDL |
Sequence similarities
Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length339
- Mass (Da)37,153
- Last updated1993-10-01 v1
- Checksum06CDE76D2FDC24A7
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 37 | in Ref. 3; AAB05220 | ||||
Sequence: E → G | ||||||
Sequence conflict | 48 | in Ref. 3; AAB05220 | ||||
Sequence: S → T |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X68305 EMBL· GenBank· DDBJ | CAA48377.1 EMBL· GenBank· DDBJ | mRNA | ||
U09958 EMBL· GenBank· DDBJ | AAB60275.1 EMBL· GenBank· DDBJ | mRNA | ||
U38845 EMBL· GenBank· DDBJ | AAB05220.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL353865 EMBL· GenBank· DDBJ | CAB88998.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE77886.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY088028 EMBL· GenBank· DDBJ | AAM65574.1 EMBL· GenBank· DDBJ | mRNA |