P32961 · NRL1_ARATH
- ProteinNitrilase 1
- GeneNIT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids346 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Can convert indole-3-acetonitrile to the plant hormone indole-3-acetic acid.
Catalytic activity
- a nitrile + 2 H2O = a carboxylate + NH4+
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 65 | Proton acceptor | ||||
Sequence: E | ||||||
Active site | 152 | Proton donor | ||||
Sequence: K | ||||||
Active site | 186 | Nucleophile | ||||
Sequence: C |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | apoplast | |
Cellular Component | chloroplast | |
Cellular Component | cytosol | |
Cellular Component | plasmodesma | |
Molecular Function | indole-3-acetonitrile nitrilase activity | |
Molecular Function | indole-3-acetonitrile nitrile hydratase activity | |
Molecular Function | nitrilase activity | |
Biological Process | indoleacetic acid biosynthetic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNitrilase 1
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionP32961
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Initiator methionine | 1 | In isoform P32961-2; Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylserine | ||||
Sequence: S | ||||||
Modified residue | 2 | In isoform P32961-2; N-acetylserine | ||||
Sequence: S | ||||||
Chain | PRO_0000204036 | 2-346 | Nitrilase 1 | |||
Sequence: SSTKDMSTVQNATPFNGVAPSTTVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDSIVSQGGSVIISPLGQVLAGPNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLTVNEHPRKSVTFVTKVEKAEDDSNK |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in cotyledons, hypocotyls, leaves, roots, stems, flowers and siliques.
Developmental stage
Expressed throughout development, but at a very low level during the fruiting stage.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 25-297 | CN hydrolase | ||||
Sequence: VRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQGQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDSIVSQGGSVIISPLGQVLAGPNFESEGLVTADIDL |
Sequence similarities
Belongs to the carbon-nitrogen hydrolase superfamily. Nitrilase family.
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
P32961-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length346
- Mass (Da)38,152
- Last updated2008-02-05 v2
- Checksum8D4F93661CAE3C1F
P32961-2
- Name2
- NoteProduced by alternative initiation at Met-7 of isoform 1.
- Differences from canonical
- 1-6: Missing
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q8LFU8 | Q8LFU8_ARATH | NIT1 | 224 |
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_059335 | 1-6 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 312 | in Ref. 1; CAA45041 | ||||
Sequence: H → Y |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X63445 EMBL· GenBank· DDBJ | CAA45041.1 EMBL· GenBank· DDBJ | mRNA | ||
U38845 EMBL· GenBank· DDBJ | AAB05221.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Y07648 EMBL· GenBank· DDBJ | CAA68935.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL353865 EMBL· GenBank· DDBJ | CAB88999.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE77887.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE77889.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY042847 EMBL· GenBank· DDBJ | AAK68787.1 EMBL· GenBank· DDBJ | mRNA | ||
BT000040 EMBL· GenBank· DDBJ | AAN15359.1 EMBL· GenBank· DDBJ | mRNA | ||
DQ446730 EMBL· GenBank· DDBJ | ABE65989.1 EMBL· GenBank· DDBJ | mRNA |