P32863 · RAD54_YEAST
- ProteinDNA repair and recombination protein RAD54
- GeneRAD54
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids898 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays an essential role in homologous recombination (HR) which is a major pathway for repairing DNA double-strand breaks (DSBs), single-stranded DNA (ssDNA) gaps, and stalled or collapsed replication forks (PubMed:12453424).
Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism (PubMed:32502392).
Also plays an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized (PubMed:31492866).
Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA (PubMed:12453424).
Acts as a molecular motor during the homology search and guides RAD51 ssDNA along a donor dsDNA thereby changing the homology search from the diffusion-based mechanism to a motor-guided mechanism (PubMed:32502392).
Also plays an essential role in RAD51-mediated synaptic complex formation which consists of three strands encased in a protein filament formed once homology is recognized (PubMed:31492866).
Once DNA strand exchange occured, dissociates RAD51 from nucleoprotein filaments formed on dsDNA (PubMed:12453424).
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | ATP-dependent chromatin remodeler activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA translocase activity | |
Molecular Function | helicase activity | |
Molecular Function | metal ion binding | |
Biological Process | chromatin remodeling | |
Biological Process | DNA geometric change | |
Biological Process | double-strand break repair via synthesis-dependent strand annealing | |
Biological Process | heteroduplex formation | |
Biological Process | reciprocal meiotic recombination | |
Biological Process | telomere maintenance via recombination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA repair and recombination protein RAD54
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP32863
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 341 | Complete loss of ability to support homology search. | ||||
Sequence: K → R |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 16 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000074344 | 1-898 | DNA repair and recombination protein RAD54 | |||
Sequence: MARRRLPDRPPNGIGAGERPRLVPRPINVQDSVNRLTKPFRVPYKNTHIPPAAGRIATGSDNIVGGRSLRKRSATVCYSGLDINADEAEYNSQDISFSQLTKRRKDALSAQRLAKDPTRLSHIQYTLRRSFTVPIKGYVQRHSLPLTLGMKKKITPEPRPLHDPTDEFAIVLYDPSVDGEMIVHDTSMDNKEEESKKMIKSTQEKDNINKEKNSQEERPTQRIGRHPALMTNGVRNKPLRELLGDSENSAENKKKFASVPVVIDPKLAKILRPHQVEGVRFLYRCVTGLVMKDYLEAEAFNTSSEDPLKSDEKALTESQKTEQNNRGAYGCIMADEMGLGKTLQCIALMWTLLRQGPQGKRLIDKCIIVCPSSLVNNWANELIKWLGPNTLTPLAVDGKKSSMGGGNTTVSQAIHAWAQAQGRNIVKPVLIISYETLRRNVDQLKNCNVGLMLADEGHRLKNGDSLTFTALDSISCPRRVILSGTPIQNDLSEYFALLSFSNPGLLGSRAEFRKNFENPILRGRDADATDKEITKGEAQLQKLSTIVSKFIIRRTNDILAKYLPCKYEHVIFVNLKPLQNELYNKLIKSREVKKVVKGVGGSQPLRAIGILKKLCNHPNLLNFEDEFDDEDDLELPDDYNMPGSKARDVQTKYSAKFSILERFLHKIKTESDDKIVLISNYTQTLDLIEKMCRYKHYSAVRLDGTMSINKRQKLVDRFNDPEGQEFIFLLSSKAGGCGINLIGANRLILMDPDWNPAADQQALARVWRDGQKKDCFIYRFISTGTIEEKIFQRQSMKMSLSSCVVDAKEDVERLFSSDNLRQLFQKNENTICETHETYHCKRCNAQGKQLKRAPAMLYGDATTWNHLNHDALEKTNDHLLKNEHHYNDISFAFQYISH |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
Expression increases in late G1 phase compared to other phases of the cell cycle.
Interaction
Subunit
Homohexamer (By similarity).
Interacts with RAD51; RAD51-ssDNA filaments do not interact autonomously with dsDNA but do so robustly in the presence of RAD54 (PubMed:31492866, PubMed:9590697).
Interacts with RAD51; RAD51-ssDNA filaments do not interact autonomously with dsDNA but do so robustly in the presence of RAD54 (PubMed:31492866, PubMed:9590697).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P32863 | RAD51 P25454 | 2 | EBI-14728, EBI-14709 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, motif, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-22 | Disordered | ||||
Sequence: MARRRLPDRPPNGIGAGERPRL | ||||||
Motif | 21-25 | Nuclear localization signal | ||||
Sequence: RLVPR | ||||||
Motif | 41-45 | Nuclear localization signal | ||||
Sequence: RVPYK | ||||||
Compositional bias | 188-223 | Basic and acidic residues | ||||
Sequence: MDNKEEESKKMIKSTQEKDNINKEKNSQEERPTQRI | ||||||
Region | 188-238 | Disordered | ||||
Sequence: MDNKEEESKKMIKSTQEKDNINKEKNSQEERPTQRIGRHPALMTNGVRNKP | ||||||
Region | 302-321 | Disordered | ||||
Sequence: TSSEDPLKSDEKALTESQKT | ||||||
Compositional bias | 305-319 | Basic and acidic residues | ||||
Sequence: EDPLKSDEKALTESQ | ||||||
Domain | 322-504 | Helicase ATP-binding | ||||
Sequence: EQNNRGAYGCIMADEMGLGKTLQCIALMWTLLRQGPQGKRLIDKCIIVCPSSLVNNWANELIKWLGPNTLTPLAVDGKKSSMGGGNTTVSQAIHAWAQAQGRNIVKPVLIISYETLRRNVDQLKNCNVGLMLADEGHRLKNGDSLTFTALDSISCPRRVILSGTPIQNDLSEYFALLSFSNPG | ||||||
Motif | 455-458 | DEGH box | ||||
Sequence: DEGH | ||||||
Domain | 659-812 | Helicase C-terminal | ||||
Sequence: ILERFLHKIKTESDDKIVLISNYTQTLDLIEKMCRYKHYSAVRLDGTMSINKRQKLVDRFNDPEGQEFIFLLSSKAGGCGINLIGANRLILMDPDWNPAADQQALARVWRDGQKKDCFIYRFISTGTIEEKIFQRQSMKMSLSSCVVDAKEDVE |
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length898
- Mass (Da)101,754
- Last updated1993-10-01 v1
- Checksum2EC0A9B7E3D66292
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 188-223 | Basic and acidic residues | ||||
Sequence: MDNKEEESKKMIKSTQEKDNINKEKNSQEERPTQRI | ||||||
Compositional bias | 305-319 | Basic and acidic residues | ||||
Sequence: EDPLKSDEKALTESQ |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M63232 EMBL· GenBank· DDBJ | AAA34949.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z48618 EMBL· GenBank· DDBJ | CAA88534.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z72685 EMBL· GenBank· DDBJ | CAA96875.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006941 EMBL· GenBank· DDBJ | DAA07949.1 EMBL· GenBank· DDBJ | Genomic DNA |