P32746 · PYRD_ARATH

Function

function

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )
Note: Binds 1 FMN per subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
40 μMdihydroorotate
112 μMdecylubiquinone

pH Dependence

Optimum pH is 7.5.

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site141-145FMN (UniProtKB | ChEBI)
Binding site145substrate
Binding site165FMN (UniProtKB | ChEBI)
Binding site190-194substrate
Binding site243FMN (UniProtKB | ChEBI)
Binding site274FMN (UniProtKB | ChEBI)
Binding site274-279substrate
Active site277Nucleophile
Binding site319FMN (UniProtKB | ChEBI)
Binding site347FMN (UniProtKB | ChEBI)
Binding site348-349substrate
Binding site371FMN (UniProtKB | ChEBI)
Binding site400FMN (UniProtKB | ChEBI)
Binding site421-422FMN (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmitochondrial inner membrane
Cellular Componentmitochondrion
Cellular Componentplastid
Molecular Functiondihydroorotate dehydrogenase (quinone) activity
Molecular Functiondihydroorotate dehydrogenase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processpyrimidine ribonucleotide biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dihydroorotate dehydrogenase (quinone), mitochondrial
  • EC number
  • Short names
    DHOdehase
  • Alternative names
    • Dihydroorotate oxidase

Gene names

    • Name
      PYRD
    • ORF names
      MKD15.16
    • Ordered locus names
      At5g23300

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    P32746
  • Secondary accessions
    • Q0WRX8
    • Q9FMX1

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane53-69Helical

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-32Mitochondrion
ChainPRO_000002988933-460Dihydroorotate dehydrogenase (quinone), mitochondrial

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias.

TypeIDPosition(s)Description
Region213-245Disordered
Compositional bias215-229Polar residues

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    460
  • Mass (Da)
    48,547
  • Last updated
    2002-12-06 v2
  • Checksum
    E8DF1C6CCEA44FFD
MAGRAATSSAKWAREFLFRRVSSNPLGATRNCSSVPGASSAPKVPHFSKRGRILTGATIGLAIAGGAYVSTADEATFCGWLFNATKVVNPFFALLDAEFAHKLAVSAAARGWVPREKRPDPAILGLEVWGRKFSNPIGLAAGFDKNAEATEGLLGMGFGFVEVGSVTPVPQEGNPKPRIFRLSQEGAIINRCGFNSEGIVVVAKRLGAQHGKRMLAETSATSSSPSDDVKPGGKSGPGILGVNLGKNKTSEDAAADYVQGVHNLSQYADYLVINVSSPNTAGLRMLQGRKQLKDLVKKVQAARDEMQWGDEGPPPLLVKIAPDLSRGELEDIAAVALALHLDGLIISNTTVSRPDAVSNNPVATETGGLSGKPLFALSTNMLRDMYTLTRGKIPLIGCGGVSSGEDAYKKIRAGATLVQLYTGFAYGGPALIPQIKEELVKCLERDGFKSIHEAIGADHR

Sequence caution

The sequence BAB11185.1 differs from that shown. Reason: Erroneous gene model prediction
The sequence CAA44695.1 differs from that shown. Reason: Frameshift

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias215-229Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X62909
EMBL· GenBank· DDBJ
CAA44695.1
EMBL· GenBank· DDBJ
mRNA Frameshift
AF454729
EMBL· GenBank· DDBJ
AAN64025.1
EMBL· GenBank· DDBJ
mRNA
AB007648
EMBL· GenBank· DDBJ
BAB11185.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002688
EMBL· GenBank· DDBJ
AED93149.1
EMBL· GenBank· DDBJ
Genomic DNA
AK228165
EMBL· GenBank· DDBJ
BAF00121.1
EMBL· GenBank· DDBJ
mRNA
BT026524
EMBL· GenBank· DDBJ
ABH04631.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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