P32569 · MED17_YEAST

Function

function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.

Miscellaneous

Present with 1720 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcore mediator complex
Cellular Componentmediator complex
Cellular Componentnucleus
Molecular FunctionDNA-binding transcription factor binding
Molecular FunctionRNA polymerase II complex recruiting activity
Molecular FunctionRNA polymerase II core promoter sequence-specific DNA binding
Molecular Functiontranscription coregulator activity
Biological Processcellular response to heat
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processpositive regulation of transcription elongation by RNA polymerase II
Biological Processpositive regulation of transcription initiation by RNA polymerase II
Biological Processregulation of transcription by RNA polymerase II
Biological ProcessRNA polymerase II preinitiation complex assembly
Biological Processtranscription initiation at RNA polymerase II promoter

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Mediator of RNA polymerase II transcription subunit 17
  • Alternative names
    • Mediator complex subunit 17
    • Suppressor of RNA polymerase B 4

Gene names

    • Name
      SRB4
    • Synonyms
      MED17
    • Ordered locus names
      YER022W

Organism names

Accessions

  • Primary accession
    P32569
  • Secondary accessions
    • D3DLS1
    • Q06790
    • Q6B1B4

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis353In SRB4-1; suppresses the phenotypic defects of an RNA polymerase IICTD truncation.

Variants

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The viewer provides 15 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000963661-687Mediator of RNA polymerase II transcription subunit 17

Proteomic databases

PTM databases

Interaction

Subunit

Component of the Mediator complex, which is composed of at least 21 subunits that form three structurally distinct submodules. The Mediator head module contains MED6, MED8, MED11, SRB4/MED17, SRB5/MED18, ROX3/MED19, SRB2/MED20 and SRB6/MED22, the middle module contains MED1, MED4, NUT1/MED5, MED7, CSE2/MED9, NUT2/MED10, SRB7/MED21 and SOH1/MED31, and the tail module contains MED2, PGD1/MED3, RGR1/MED14, GAL11/MED15 and SIN4/MED16. The head and the middle modules interact directly with RNA polymerase II, whereas the elongated tail module interacts with gene-specific regulatory proteins. The head module may also interact with the TFIIF complex. SRB4/MED17 interacts directly with MED6, MED11, ROX3/MED19, SRB2/MED20 and SRB6/MED22. Interacts directly with the activator GAL4.

Binary interactions

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias30-44Polar residues
Region30-70Disordered
Compositional bias56-70Basic and acidic residues
Region130-189Disordered
Compositional bias136-151Basic and acidic residues
Compositional bias166-187Basic and acidic residues

Sequence similarities

Belongs to the Mediator complex subunit 17 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    687
  • Mass (Da)
    78,476
  • Last updated
    1993-10-01 v1
  • Checksum
    D25B2993A1BADFD6
MTTEDPDSNHLSSETGIKLALDPNLITLALSSNPNSSLHSPTSDEPVPESAGKADTSIRLEGDELENKTKKDNDKNLKFLKNKDSLVSNPHEIYGSMPLEQLIPIILRQRGPGFKFVDLNEKELQNEIKQLGSDSSDGHNSEKKDTDGADENVQIGEDFMEVDYEDKDNPVDSRNETDHKTNENGETDDNIETVMTQEQFVKRRRDMLEHINLAMNESSLALEFVSLLLSSVKESTGMSSMSPFLRKVVKPSSLNSDKIPYVAPTKKEYIELDILNKGWKLQSLNESKDLLRASFNKLSSILQNEHDYWNKIMQSISNKDVIFKIRDRTSGQKLLAIKYGYEDSGSTYKHDRGIANIRNNIESQNLDLIPHSSSVFKGTDFVHSVKKFLRVRIFTKIESEDDYILSGESVMDRDSESEEAETKDIRKQIQLLKKIIFEKELMYQIKKECALLISYGVSIENENKVIIELPNEKFEIELLSLDDDSIVNHEQDLPKINDKRANLMLVMLRLLLVVIFKKTLRSRISSPHGLINLNVDDDILIIRPILGKVRFANYKLLLKKIIKDYVLDIVPGSSITETEVEREQPQENKNIDDENITKLNKEIRAFDKLLNIPRRELKINLPLTEHKSPNLSLMLESPNYCNALIHIKFSAGTEANAVSFDTTFSDFKEVEDFLHFIVAEYIQQKKV

Features

Showing features for compositional bias, sequence conflict.

TypeIDPosition(s)Description
Compositional bias30-44Polar residues
Compositional bias56-70Basic and acidic residues
Compositional bias136-151Basic and acidic residues
Compositional bias166-187Basic and acidic residues
Sequence conflict430-431in Ref. 5; CAA40613
Sequence conflict647in Ref. 4; AAT93185

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L12026
EMBL· GenBank· DDBJ
AAA02632.1
EMBL· GenBank· DDBJ
Unassigned DNA
U18778
EMBL· GenBank· DDBJ
AAB64555.1
EMBL· GenBank· DDBJ
Genomic DNA
AY693166
EMBL· GenBank· DDBJ
AAT93185.1
EMBL· GenBank· DDBJ
Genomic DNA
X57338
EMBL· GenBank· DDBJ
CAA40613.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006939
EMBL· GenBank· DDBJ
DAA07675.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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