P32524 · PRP21_YEAST

Function

function

mRNA splicing factors, PRP9, PRP11, and PRP21, are necessary for binding of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly.

Miscellaneous

Present with 2490 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcatalytic step 2 spliceosome
Cellular Componentnucleus
Cellular Componentspliceosomal complex
Cellular ComponentU2 snRNP
Cellular ComponentU2-type prespliceosome
Molecular FunctionRNA binding
Biological ProcessmRNA cis splicing, via spliceosome
Biological ProcessmRNA splicing, via spliceosome
Biological ProcessU2-type prespliceosome assembly

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pre-mRNA-splicing factor PRP21

Gene names

    • Name
      PRP21
    • Synonyms
      SPP91
    • ORF names
      J0322
    • Ordered locus names
      YJL203W

Organism names

Accessions

  • Primary accession
    P32524
  • Secondary accessions
    • D6VVZ1
    • Q6Q5G7

Proteomes

Organism-specific databases

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis168In SPP91-1; corrects the PRP9-1 growth defect through partial restoration of splicing and by a complete reversion of the pre-mRNA escape phenotype.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 8 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001743221-280Pre-mRNA-splicing factor PRP21

Proteomic databases

PTM databases

Interaction

Subunit

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P32524PRP11 Q073508EBI-603, EBI-688

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for repeat, region, compositional bias.

TypeIDPosition(s)Description
Repeat11-49SURP motif 1
Region53-72Disordered
Repeat95-135SURP motif 2
Region246-280Disordered
Compositional bias259-280Basic residues

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    280
  • Mass (Da)
    33,052
  • Last updated
    1993-10-01 v1
  • Checksum
    2E9DBA6F06759B3F
MEPEDTQLKEDIKTTVNYIKQHGVEFENKLLEDERFSFIKKDDPLHEYYTKLMNEPTDTVSGEDNDRKSEREIARPPDFLFSQYDTGISRRDMEVIKLTARYYAKDKSIVEQMISKDGEARLNFMNSSHPLHKTFTDFVAQYKRVYSFTGQEIKKSKRTILDNCFERTQYWEFEKDKDREHDKLVELCKIQFAAIPWDKFTQVAKFSIPEDTEIFEGSLDLEQMRLRRVQTGIKLFDSIKPTNEEEKIVSDQGKQKGGDSKGKKRKIRAVGETRLKKSKK

Features

Showing features for sequence conflict, compositional bias.

TypeIDPosition(s)Description
Sequence conflict205in Ref. 6; AAS56405
Compositional bias259-280Basic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X67564
EMBL· GenBank· DDBJ
CAA47860.1
EMBL· GenBank· DDBJ
Genomic DNA
L07744
EMBL· GenBank· DDBJ
AAB09601.1
EMBL· GenBank· DDBJ
Genomic DNA
X77688
EMBL· GenBank· DDBJ
CAA54754.1
EMBL· GenBank· DDBJ
Genomic DNA
Z49478
EMBL· GenBank· DDBJ
CAA89497.1
EMBL· GenBank· DDBJ
Genomic DNA
AY558079
EMBL· GenBank· DDBJ
AAS56405.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006943
EMBL· GenBank· DDBJ
DAA08607.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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