P32171 · RHAB_ECOLI

Function

function

Involved in the catabolism of L-rhamnose (6-deoxy-L-mannose). It could also play a role in the metabolism of some rare sugars such as L-fructose. Catalyzes the transfer of the gamma-phosphate group from ATP to the 1-hydroxyl group of L-rhamnulose to yield L-rhamnulose 1-phosphate. Uridine triphosphate (UTP), cytidine 5-triphosphate (CTP), guanosine 5-triphosphate (GTP), and thymidine triphosphate (TTP) also can act as phosphoryl donors. It can also phosphorylate L-fuculose and L-xylulose.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: It can also use manganese, cobalt, iron, calcium and copper ions.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
82 μML-rhamnulose8.537
110 μMATP8.537
270 μMmagnesium ion8.537
3 mMbeta-L-fructose8

pH Dependence

Optimum pH is 8.5.

Pathway

Carbohydrate degradation; L-rhamnose degradation; glycerone phosphate from L-rhamnose: step 2/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site13-17ATP (UniProtKB | ChEBI)
Binding site83substrate
Binding site236-238substrate
Active site237Proton acceptor
Binding site259ATP (UniProtKB | ChEBI)
Binding site296substrate
Binding site304ATP (UniProtKB | ChEBI)
Binding site402ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular Functionglycerol kinase activity
Molecular Functionrhamnulokinase activity
Biological Processglycerol metabolic process
Biological Processrhamnose catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    L-Rhamnulokinase
  • EC number
  • Short names
    RhaB
    ; RhuK
  • Alternative names
    • ATP:L-rhamnulose phosphotransferase
    • L-rhamnulose 1-kinase
    • Rhamnulose kinase

Gene names

    • Name
      rhaB
    • Ordered locus names
      b3904, JW3875

Organism names

  • Taxonomic identifier
  • Strains
    • K12
    • K12 / MG1655 / ATCC 47076
    • K12 / W3110 / ATCC 27325 / DSM 5911
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P32171
  • Secondary accessions
    • Q2M8K1

Proteomes

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene are unable to utilize rhamnose as a source of carbon.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis69Same kinase activity as the wild-type; when associated with A-70 and A-73.
Mutagenesis70Same kinase activity as the wild-type; when associated with A-69 and A-73.
Mutagenesis73Same kinase activity as the wild-type; when associated with A-69 and A-70.

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00000905311-489L-Rhamnulokinase
Disulfide bond68↔222
Disulfide bond353↔370
Disulfide bond413↔417

Keywords

Proteomic databases

Expression

Induction

Induced by L-rhamnose via the RhaR-RhaS regulatory cascade. Binding of the cAMP receptor protein (CRP) is required for full expression. Also induced by L-lyxose.

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the rhamnulokinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    489
  • Mass (Da)
    54,069
  • Last updated
    1993-10-01 v1
  • Checksum
    AF66259EACAC5F4E
MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHSQNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAYRDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLMPDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIGHWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTALAANITNEGGAEGRYRVLKNIMGLWLLQRVLQEQQINDLPALISATQALPACRFIINPNDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGEDFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA

Sequence caution

The sequence CAA43001.1 differs from that shown. Reason: Frameshift

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict214in Ref. 1; CAA43001
Sequence conflict388-389in Ref. 1; CAA43001

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X60472
EMBL· GenBank· DDBJ
CAA43001.1
EMBL· GenBank· DDBJ
Genomic DNA Frameshift
L19201
EMBL· GenBank· DDBJ
AAB03037.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC76886.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAE77405.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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