P31813 · RPO1N_THECE
- ProteinDNA-directed RNA polymerase subunit Rpo1N
- Generpo1N
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids905 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Protein has several cofactor binding sites:
Note: Binds at least 2 Zn2+ per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 60 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 63 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 70 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 73 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 100 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 103 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 147 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 150 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 461 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 463 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 465 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit Rpo1N
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Thermococci > Thermococcales > Thermococcaceae > Thermococcus
Accessions
- Primary accessionP31813
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000074010 | 1-905 | DNA-directed RNA polymerase subunit Rpo1N | |||
Sequence: MQSIKKVIGSIDFGILSPQEIRKMSAAEITVPDTYDEDGYPIEGGLMDKRLGVIDPGLRCETCGARAGECPGHFGHVELARPVIHVGFAKTIHRVLESTCRECGRIKLTDEEIEEYFHKFEVTGNRKKAKDRLIKEIHKKAKERMVCPHCGSPQFPIKFERPTVYWELRKDEEGNEYKHRMMPSEVRDRLEKIPDRDLPLIGLDAEKARPEWMVLTVLPVPPVTMRPSITLESGIRAEDDLTHKLVDIIRINNRLKSNIEAGAPQLIIEDLWDLLQYHVTTYINNETSGIPPAKHKSGRPLKTLSQRLKGKEGRFRGNLSGKRVNFSARTVISPDPMISINEVGVPMAIAMELTVPEKVTEFNFEKLRKMVLNGPEKYPGANYVIDPEGRRIRLMENNLETVAEKLDIGWTVERHLLDGDVVLFNRQPSLHRMSIMAHRVRVMPYRTFRLNLSVCPPYNADFDGDEMNLHVPQTEEAQAEAKILMEVQNHIISPRYGGPLIAGIQDHISGGYLLTREGAYFERTEVEQMLMFAGVDVDRLPEPDKVENGVELWSGKTIFSLLLPKDLTIWYRNKLCDEPGRCEALEKLIEEKLIPDEEEVRALAYDGFTYILNGKLLSGAIDKTAYGREDGKLLDIIVREYGVERARQFLDQVTKLAIWVITHKGFTTAIDDEDLPTEALDRIREIIREAEERVNRLIEAYRNGELEPIPGKTLEETLESKIMAALAEARDNAGKVAERYLGMSNHAVIMAKTGARGKILNITQMAAMLGQQSIRGKRLYRGYRERVLTHFKPGDLGARARGFVINSYKSGLTPQEYFFHAMGGREGLVDTAVRTAQSGYMQRRLINALQDLKVEYDGTVRDPTGIIVQFRYGEDGIDPMRSWKGKTIDVNRVVLRTLLKLRGKG |
Interaction
Subunit
Part of the RNA polymerase complex.
Structure
Sequence
- Sequence statusComplete
- Length905
- Mass (Da)102,725
- Last updated1993-07-01 v1
- Checksum9B71DC59BCF9A9AA