P31813 · RPO1N_THECE

  • Protein
    DNA-directed RNA polymerase subunit Rpo1N
  • Gene
    rpo1N
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds at least 2 Zn2+ per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site60Zn2+ 1 (UniProtKB | ChEBI)
Binding site63Zn2+ 1 (UniProtKB | ChEBI)
Binding site70Zn2+ 1 (UniProtKB | ChEBI)
Binding site73Zn2+ 1 (UniProtKB | ChEBI)
Binding site100Zn2+ 2 (UniProtKB | ChEBI)
Binding site103Zn2+ 2 (UniProtKB | ChEBI)
Binding site147Zn2+ 2 (UniProtKB | ChEBI)
Binding site150Zn2+ 2 (UniProtKB | ChEBI)
Binding site461Mg2+ (UniProtKB | ChEBI)
Binding site463Mg2+ (UniProtKB | ChEBI)
Binding site465Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular ComponentDNA-directed RNA polymerase complex
Molecular FunctionDNA binding
Molecular FunctionDNA-directed 5'-3' RNA polymerase activity
Molecular Functionmagnesium ion binding
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA-directed RNA polymerase subunit Rpo1N
  • EC number
  • Alternative names
    • DNA-directed RNA polymerase subunit A'

Gene names

    • Name
      rpo1N
    • Synonyms
      rpoA1

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 35543 / DSM 2476 / JCM 8558 / Vu 13
  • Taxonomic lineage
    Archaea > Euryarchaeota > Thermococci > Thermococcales > Thermococcaceae > Thermococcus

Accessions

  • Primary accession
    P31813

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000740101-905DNA-directed RNA polymerase subunit Rpo1N

Interaction

Subunit

Part of the RNA polymerase complex.

Structure

Family & Domains

Sequence similarities

Belongs to the RNA polymerase beta' chain family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    905
  • Mass (Da)
    102,725
  • Last updated
    1993-07-01 v1
  • Checksum
    9B71DC59BCF9A9AA
MQSIKKVIGSIDFGILSPQEIRKMSAAEITVPDTYDEDGYPIEGGLMDKRLGVIDPGLRCETCGARAGECPGHFGHVELARPVIHVGFAKTIHRVLESTCRECGRIKLTDEEIEEYFHKFEVTGNRKKAKDRLIKEIHKKAKERMVCPHCGSPQFPIKFERPTVYWELRKDEEGNEYKHRMMPSEVRDRLEKIPDRDLPLIGLDAEKARPEWMVLTVLPVPPVTMRPSITLESGIRAEDDLTHKLVDIIRINNRLKSNIEAGAPQLIIEDLWDLLQYHVTTYINNETSGIPPAKHKSGRPLKTLSQRLKGKEGRFRGNLSGKRVNFSARTVISPDPMISINEVGVPMAIAMELTVPEKVTEFNFEKLRKMVLNGPEKYPGANYVIDPEGRRIRLMENNLETVAEKLDIGWTVERHLLDGDVVLFNRQPSLHRMSIMAHRVRVMPYRTFRLNLSVCPPYNADFDGDEMNLHVPQTEEAQAEAKILMEVQNHIISPRYGGPLIAGIQDHISGGYLLTREGAYFERTEVEQMLMFAGVDVDRLPEPDKVENGVELWSGKTIFSLLLPKDLTIWYRNKLCDEPGRCEALEKLIEEKLIPDEEEVRALAYDGFTYILNGKLLSGAIDKTAYGREDGKLLDIIVREYGVERARQFLDQVTKLAIWVITHKGFTTAIDDEDLPTEALDRIREIIREAEERVNRLIEAYRNGELEPIPGKTLEETLESKIMAALAEARDNAGKVAERYLGMSNHAVIMAKTGARGKILNITQMAAMLGQQSIRGKRLYRGYRERVLTHFKPGDLGARARGFVINSYKSGLTPQEYFFHAMGGREGLVDTAVRTAQSGYMQRRLINALQDLKVEYDGTVRDPTGIIVQFRYGEDGIDPMRSWKGKTIDVNRVVLRTLLKLRGKG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X67313
EMBL· GenBank· DDBJ
CAA47723.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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