P30656 · PSB5_YEAST

Function

function

The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib.
This subunit is necessary for chymotryptic activity and degradation of ubiquitinated proteins.

Miscellaneous

The side chain of Thr-76 acts as a nucleophile, and the N-terminal amino group acts as a proton acceptor.
Present with 8530 molecules/cell in log phase SD medium.

Catalytic activity

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site76Nucleophile
Binding site124bortezomib (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentnucleus
Cellular Componentperoxisome
Cellular Componentproteasome complex
Cellular Componentproteasome core complex, beta-subunit complex
Molecular Functionendopeptidase activity
Molecular Functionthreonine-type endopeptidase activity
Biological Processproteasomal ubiquitin-independent protein catabolic process
Biological Processproteasome core complex assembly
Biological Processproteasome-mediated ubiquitin-dependent protein catabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Proteasome subunit beta type-5
  • EC number
  • Alternative names
    • Macropain subunit PRE2
    • Multicatalytic endopeptidase complex subunit PRE2
    • Proteasome component PRE2
    • Proteinase YSCE subunit PRE2

Gene names

    • Name
      PRE2
    • Synonyms
      DOA3, PRG1
    • ORF names
      P8283.10
    • Ordered locus names
      YPR103W

Organism names

Accessions

  • Primary accession
    P30656
  • Secondary accessions
    • D6W4A1

Proteomes

Organism-specific databases

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis126In DOA3-1; decrease in activity.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 1 variant from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Chemistry

PTM/Processing

Features

Showing features for propeptide, chain.

TypeIDPosition(s)Description
PropeptidePRO_00000266111-75Removed in mature form
ChainPRO_000002661276-287Proteasome subunit beta type-5

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

Binary interactions

Protein-protein interaction databases

Chemistry

Miscellaneous

Family & Domains

Sequence similarities

Belongs to the peptidase T1B family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    287
  • Mass (Da)
    31,637
  • Last updated
    1995-02-01 v3
  • Checksum
    D0EBAC611F7A4F37
MQAIADSFSVPNRLVKELQYDNEQNLESDFVTGASQFQRLAPSLTVPPIASPQQFLRAHTDDSRNPDCKIKIAHGTTTLAFRFQGGIIVAVDSRATAGNWVASQTVKKVIEINPFLLGTMAGGAADCQFWETWLGSQCRLHELREKERISVAAASKILSNLVYQYKGAGLSMGTMICGYTRKEGPTIYYVDSDGTRLKGDIFCVGSGQTFAYGVLDSNYKWDLSVEDALYLGKRSILAAAHRDAYSGGSVNLYHVTEDGWIYHGNHDVGELFWKVKEEEGSFNNVIG

Sequence caution

The sequence CAA82203.1 differs from that shown. Reason: Erroneous initiation

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict282-287in Ref. 1; AAA34906

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M96667
EMBL· GenBank· DDBJ
AAA34906.1
EMBL· GenBank· DDBJ
Genomic DNA
X68662
EMBL· GenBank· DDBJ
CAA48628.1
EMBL· GenBank· DDBJ
Genomic DNA
U32445
EMBL· GenBank· DDBJ
AAB68073.1
EMBL· GenBank· DDBJ
Genomic DNA
Z28348
EMBL· GenBank· DDBJ
CAA82203.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
BK006949
EMBL· GenBank· DDBJ
DAA11517.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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