P29539 · RIF1_YEAST
- ProteinTelomere length regulator protein RIF1
- GeneRIF1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1916 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Negatively regulates telomere length by preventing telomere elongation or promoting degradation of the telomere ends. Recruited to telomeres by interaction with the C-terminus of RAP1, which binds directly to telomeric repeat DNA. This may create a negative feedback loop in which the addition of new telomere repeats creates binding sites for inhibitors of telomere length extension. May also influence the balance of transcriptional silencing at telomeres and the silent mating type locus HMR, which is mediated by SIR (Silent Information Regulator) proteins including SIR3 and SIR4. RIF1 competes with SIR proteins for binding to the C-terminus of RAP1. In the absence of RIF1, a limiting cellular pool of SIR proteins may preferentially associate with RAP1 at sub-telomeric loci, causing enhanced telomeric silencing and attenuated silencing of the HMR locus.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chromosome, telomeric region | |
Cellular Component | nucleus | |
Cellular Component | shelterin complex | |
Molecular Function | centromeric DNA binding | |
Molecular Function | DNA replication origin binding | |
Molecular Function | RNA polymerase II core promoter sequence-specific DNA binding | |
Molecular Function | telomeric DNA binding | |
Biological Process | DNA double-strand break processing | |
Biological Process | DNA replication initiation | |
Biological Process | negative regulation of DNA-templated DNA replication initiation | |
Biological Process | negative regulation of mitotic DNA replication initiation from late origin | |
Biological Process | protein localization to chromosome, telomeric region | |
Biological Process | regulation of DNA stability | |
Biological Process | silent mating-type cassette heterochromatin formation | |
Biological Process | telomere capping | |
Biological Process | telomere maintenance |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTelomere length regulator protein RIF1
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP29539
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes to telomeres. Telomere association begins in G1/S, attains a peak during late G2/S phase of the cell cycle, and is lost during telophase. Localization to telomeres may be increased by telomere uncapping caused by expression of a mutant telomerase RNA subunit (TLC1).
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000097336 | 1-1916 | Telomere length regulator protein RIF1 | ||
Modified residue | 97 | Phosphoserine | |||
Modified residue | 1637 | Phosphoserine | |||
Modified residue | 1795 | Phosphoserine | |||
Modified residue | 1852 | Phosphoserine | |||
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with RAP1 (via C-terminus) (PubMed:1577274, PubMed:7867933, PubMed:9087429).
Interacts with RIF2 (PubMed:7867933, PubMed:9087429).
Interacts with RIF2 (PubMed:7867933, PubMed:9087429).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | P29539 | RAP1 P11938 | 3 | EBI-2083307, EBI-14821 |
Complex viewer
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-24 | Basic and acidic residues | |||
Region | 1-71 | Disordered | |||
Compositional bias | 46-64 | Polar residues | |||
Region | 126-149 | Disordered | |||
Compositional bias | 1314-1357 | Polar residues | |||
Region | 1314-1372 | Disordered | |||
Compositional bias | 1441-1461 | Polar residues | |||
Region | 1441-1476 | Disordered | |||
Compositional bias | 1462-1476 | Basic and acidic residues | |||
Region | 1606-1678 | Disordered | |||
Compositional bias | 1627-1647 | Basic and acidic residues | |||
Compositional bias | 1650-1668 | Polar residues | |||
Region | 1789-1811 | Disordered | |||
Compositional bias | 1797-1811 | Polar residues | |||
Region | 1829-1854 | Disordered | |||
Sequence similarities
Belongs to the RIF1 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,916
- Mass (Da)217,931
- Last updated2011-07-27 v3
- Checksum07070AF02361D96F
Features
Showing features for compositional bias, sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-24 | Basic and acidic residues | |||
Compositional bias | 46-64 | Polar residues | |||
Sequence conflict | 580 | in Ref. 1; CAA47121 | |||
Sequence conflict | 732 | in Ref. 2; CAA85238 and 4; CAA53638 | |||
Compositional bias | 1314-1357 | Polar residues | |||
Compositional bias | 1441-1461 | Polar residues | |||
Compositional bias | 1462-1476 | Basic and acidic residues | |||
Compositional bias | 1627-1647 | Basic and acidic residues | |||
Compositional bias | 1650-1668 | Polar residues | |||
Compositional bias | 1797-1811 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X66501 EMBL· GenBank· DDBJ | CAA47121.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z36144 EMBL· GenBank· DDBJ | CAA85238.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z36145 EMBL· GenBank· DDBJ | CAA85240.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X76053 EMBL· GenBank· DDBJ | CAA53638.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006936 EMBL· GenBank· DDBJ | DAA07391.2 EMBL· GenBank· DDBJ | Genomic DNA |