P29340 · UBCX_YEAST

Function

function

Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis (By similarity) (PubMed:17550898, PubMed:18644345).
Required for UBC4-independent ubiquitination of PEX5 (PubMed:17550898, PubMed:18644345).

Catalytic activity

  • S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.
    EC:2.3.2.23 (UniProtKB | ENZYME | Rhea)

Pathway

Protein modification; protein ubiquitination.

Features

Showing features for active site.

118320406080100120140160180
TypeIDPosition(s)Description
Active site115Glycyl thioester intermediate

GO annotations

AspectTerm
Cellular Componentnucleus
Cellular Componentperoxisome
Molecular FunctionATP binding
Molecular Functionubiquitin conjugating enzyme activity
Molecular Functionubiquitin-protein transferase activity
Biological Processperoxisome organization
Biological Processprotein autoubiquitination
Biological Processprotein import into peroxisome matrix, receptor recycling
Biological Processprotein monoubiquitination
Biological Processprotein polyubiquitination
Biological Processprotein ubiquitination

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Ubiquitin-conjugating enzyme E2-21 kDa
  • EC number
  • Alternative names
    • E2 ubiquitin-conjugating enzyme PEX4
    • Peroxin-4
    • Ubiquitin carrier protein
    • Ubiquitin-protein ligase

Gene names

    • Name
      PEX4
    • Synonyms
      PAS2, UBC10
    • Ordered locus names
      YGR133W

Organism names

Accessions

  • Primary accession
    P29340
  • Secondary accessions
    • D6VUR5

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000825581-183Ubiquitin-conjugating enzyme E2-21 kDa

Proteomic databases

Interaction

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY P29340PEX22 P3971810EBI-19784, EBI-20707

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain17-179UBC core

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    183
  • Mass (Da)
    21,118
  • Last updated
    1992-12-01 v1
  • Checksum
    D4E438B689F76CAD
MPNFWILENRRSYTSDTCMSRIVKEYKVILKTLASDDPIANPYRGIIESLNPIDETDLSKWEAIISGPSDTPYENHQFRILIEVPSSYPMNPPKISFMQNNILHCNVKSATGEICLNILKPEEWTPVWDLLHCVHAVWRLLREPVCDSPLDVDIGNIIRCGDMSAYQGIVKYFLAERERINNH

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X65470
EMBL· GenBank· DDBJ
CAA46467.1
EMBL· GenBank· DDBJ
Genomic DNA
Z72918
EMBL· GenBank· DDBJ
CAA97146.1
EMBL· GenBank· DDBJ
Genomic DNA
AY557776
EMBL· GenBank· DDBJ
AAS56102.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006941
EMBL· GenBank· DDBJ
DAA08226.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp