P29340 · UBCX_YEAST
- ProteinUbiquitin-conjugating enzyme E2-21 kDa
- GenePEX4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids183 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the covalent attachment of ubiquitin to other proteins. Essential for peroxisome biogenesis (By similarity) (PubMed:17550898, PubMed:18644345).
Required for UBC4-independent ubiquitination of PEX5 (PubMed:17550898, PubMed:18644345).
Required for UBC4-independent ubiquitination of PEX5 (PubMed:17550898, PubMed:18644345).
Catalytic activity
Pathway
Protein modification; protein ubiquitination.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 115 | Glycyl thioester intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Cellular Component | peroxisome | |
Molecular Function | ATP binding | |
Molecular Function | ubiquitin conjugating enzyme activity | |
Molecular Function | ubiquitin-protein transferase activity | |
Biological Process | peroxisome organization | |
Biological Process | protein autoubiquitination | |
Biological Process | protein import into peroxisome matrix, receptor recycling | |
Biological Process | protein monoubiquitination | |
Biological Process | protein polyubiquitination | |
Biological Process | protein ubiquitination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameUbiquitin-conjugating enzyme E2-21 kDa
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP29340
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000082558 | 1-183 | Ubiquitin-conjugating enzyme E2-21 kDa | |||
Sequence: MPNFWILENRRSYTSDTCMSRIVKEYKVILKTLASDDPIANPYRGIIESLNPIDETDLSKWEAIISGPSDTPYENHQFRILIEVPSSYPMNPPKISFMQNNILHCNVKSATGEICLNILKPEEWTPVWDLLHCVHAVWRLLREPVCDSPLDVDIGNIIRCGDMSAYQGIVKYFLAERERINNH |
Proteomic databases
Interaction
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P29340 | PEX22 P39718 | 10 | EBI-19784, EBI-20707 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 17-179 | UBC core | ||||
Sequence: TCMSRIVKEYKVILKTLASDDPIANPYRGIIESLNPIDETDLSKWEAIISGPSDTPYENHQFRILIEVPSSYPMNPPKISFMQNNILHCNVKSATGEICLNILKPEEWTPVWDLLHCVHAVWRLLREPVCDSPLDVDIGNIIRCGDMSAYQGIVKYFLAERER |
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length183
- Mass (Da)21,118
- Last updated1992-12-01 v1
- ChecksumD4E438B689F76CAD
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X65470 EMBL· GenBank· DDBJ | CAA46467.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z72918 EMBL· GenBank· DDBJ | CAA97146.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY557776 EMBL· GenBank· DDBJ | AAS56102.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006941 EMBL· GenBank· DDBJ | DAA08226.1 EMBL· GenBank· DDBJ | Genomic DNA |