P29168 · GAG_MLVDE
- ProteinGag polyprotein
- Genegag
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids536 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Gag polyprotein
Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release.
Matrix protein p15
Targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the pre-integration complex.
RNA-binding phosphoprotein p12
Constituent of the pre-integration complex (PIC) which tethers the latter to mitotic chromosomes.
Capsid protein p30
Forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.
Nucleocapsid protein p10-gag
Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization.
Features
Showing features for site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 129-130 | Cleavage; by viral protease p14 | ||||
Sequence: YT | ||||||
Site | 213-214 | Cleavage; by viral protease p14 | ||||
Sequence: FP | ||||||
Site | 476-477 | Cleavage; by viral protease p14 | ||||
Sequence: LA |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell plasma membrane | |
Cellular Component | membrane | |
Cellular Component | viral nucleocapsid | |
Molecular Function | RNA binding | |
Molecular Function | structural constituent of virion | |
Molecular Function | zinc ion binding | |
Biological Process | virion assembly |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Recommended nameGag polyprotein
- Alternative names
- Cleaved into 4 chains
Gene names
Organism names
- Taxonomic lineageViruses > Riboviria > Pararnavirae > Artverviricota > Revtraviricetes > Ortervirales > Retroviridae > Orthoretrovirinae > Gammaretrovirus > Murine leukemia virus
- Virus hosts
Accessions
- Primary accessionP29168
Subcellular Location
UniProt Annotation
GO Annotation
Gag polyprotein
Host cell membrane ; Lipid-anchor
Matrix protein p15
Capsid protein p30
Nucleocapsid protein p10-gag
Keywords
- Cellular component
PTM/Processing
Features
Showing features for initiator methionine, lipidation, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed; by host | ||||
Sequence: M | ||||||
Lipidation | 2 | N-myristoyl glycine; by host | ||||
Sequence: G | ||||||
Chain | PRO_0000040888 | 2-129 | Matrix protein p15 | |||
Sequence: GQTITTPLSLTLEHWRDVQCIASNQSVDVKRRRWVTFCSVEWPSFDVGWPLDGTFNLDIILQVKSKVFCPGPHGHPDQVPYIVTWEALAYHPPPWVKPFVSPKPFPLSTLPFSPPGPSAHPPSRSDLY | ||||||
Chain | PRO_0000390809 | 2-536 | Gag polyprotein | |||
Sequence: GQTITTPLSLTLEHWRDVQCIASNQSVDVKRRRWVTFCSVEWPSFDVGWPLDGTFNLDIILQVKSKVFCPGPHGHPDQVPYIVTWEALAYHPPPWVKPFVSPKPFPLSTLPFSPPGPSAHPPSRSDLYTALIPSIKTKPPKSRVLPTNGGPLIDLLTENPPNLGEQGPPLPKGPVKKRRPPPPRYSPPGPMVSRLRGNRDPPAADSTTSRAFPLRLGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLTTHQPTWDDCQQLLGTLLTGEEKQRVLLEARKAVRGNDGRPTQLPNEVNSAFPLERPDWDYTTPEGRNHLVLYRQLLLAGLQNAGRSPTNLAKVKGITQGPSESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIWQSAPDIGRKLERLEDLKSKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRAEDEQREKERDRRRHREMSKFLATVVTGQRQDRQGGERRRPQLDKDQCAYCKEKGHWAKDCPKKPRGPRGPRPQTSLLTLGD | ||||||
Chain | PRO_0000040889 | 130-213 | RNA-binding phosphoprotein p12 | |||
Sequence: TALIPSIKTKPPKSRVLPTNGGPLIDLLTENPPNLGEQGPPLPKGPVKKRRPPPPRYSPPGPMVSRLRGNRDPPAADSTTSRAF | ||||||
Chain | PRO_0000040890 | 214-476 | Capsid protein p30 | |||
Sequence: PLRLGGNGQLQYWPFSSSDLYNWKNNNPSFSEDPGKLTALIESVLTTHQPTWDDCQQLLGTLLTGEEKQRVLLEARKAVRGNDGRPTQLPNEVNSAFPLERPDWDYTTPEGRNHLVLYRQLLLAGLQNAGRSPTNLAKVKGITQGPSESPSAFLERLKEAYRRYTPYDPEDPGQETNVSMSFIWQSAPDIGRKLERLEDLKSKTLGDLVREAEKIFNKRETPEEREERIRRETEEKEERRRAEDEQREKERDRRRHREMSKFL | ||||||
Chain | PRO_0000040891 | 477-536 | Nucleocapsid protein p10-gag | |||
Sequence: ATVVTGQRQDRQGGERRRPQLDKDQCAYCKEKGHWAKDCPKKPRGPRGPRPQTSLLTLGD |
Post-translational modification
Gag polyprotein
Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation.
Keywords
- PTM
Interaction
Subunit
Gag polyprotein
Interacts (via PPXY motif) with host NEDD4.
Capsid protein p30
Homohexamer. Further associates as homomultimer (By similarity).
The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (By similarity).
The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (By similarity).
Family & Domains
Features
Showing features for region, compositional bias, motif, coiled coil, zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 139-216 | Disordered | ||||
Sequence: KPPKSRVLPTNGGPLIDLLTENPPNLGEQGPPLPKGPVKKRRPPPPRYSPPGPMVSRLRGNRDPPAADSTTSRAFPLR | ||||||
Compositional bias | 165-190 | Pro residues | ||||
Sequence: GEQGPPLPKGPVKKRRPPPPRYSPPG | ||||||
Motif | 183-186 | PPXY motif | ||||
Sequence: PPRY | ||||||
Compositional bias | 432-474 | Basic and acidic residues | ||||
Sequence: RETPEEREERIRRETEEKEERRRAEDEQREKERDRRRHREMSK | ||||||
Region | 432-536 | Disordered | ||||
Sequence: RETPEEREERIRRETEEKEERRRAEDEQREKERDRRRHREMSKFLATVVTGQRQDRQGGERRRPQLDKDQCAYCKEKGHWAKDCPKKPRGPRGPRPQTSLLTLGD | ||||||
Coiled coil | 436-474 | |||||
Sequence: EEREERIRRETEEKEERRRAEDEQREKERDRRRHREMSK | ||||||
Compositional bias | 486-521 | Basic and acidic residues | ||||
Sequence: DRQGGERRRPQLDKDQCAYCKEKGHWAKDCPKKPRG | ||||||
Zinc finger | 500-517 | CCHC-type | ||||
Sequence: DQCAYCKEKGHWAKDCPK |
Domain
Gag polyprotein
Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. PPXY motif is essential for virus egress.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length536
- Mass (Da)60,891
- Last updated2007-01-23 v3
- Checksum4D990FD5C5AEF39B
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 165-190 | Pro residues | ||||
Sequence: GEQGPPLPKGPVKKRRPPPPRYSPPG | ||||||
Compositional bias | 432-474 | Basic and acidic residues | ||||
Sequence: RETPEEREERIRRETEEKEERRRAEDEQREKERDRRRHREMSK | ||||||
Compositional bias | 486-521 | Basic and acidic residues | ||||
Sequence: DRQGGERRRPQLDKDQCAYCKEKGHWAKDCPKKPRG |