P29074 · PTN4_HUMAN
- ProteinTyrosine-protein phosphatase non-receptor type 4
- GenePTPN4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids926 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Phosphatase that plays a role in immunity, learning, synaptic plasticity or cell homeostasis (PubMed:25825441, PubMed:27246854).
Regulates neuronal cell homeostasis by protecting neurons against apoptosis (PubMed:20086240).
Negatively regulates TLR4-induced interferon beta production by dephosphorylating adapter TICAM2 and inhibiting subsequent TRAM-TRIF interaction (PubMed:25825441).
Dephosphorylates also the immunoreceptor tyrosine-based activation motifs/ITAMs of the TCR zeta subunit and thereby negatively regulates TCR-mediated signaling pathway (By similarity).
May act at junctions between the membrane and the cytoskeleton
Regulates neuronal cell homeostasis by protecting neurons against apoptosis (PubMed:20086240).
Negatively regulates TLR4-induced interferon beta production by dephosphorylating adapter TICAM2 and inhibiting subsequent TRAM-TRIF interaction (PubMed:25825441).
Dephosphorylates also the immunoreceptor tyrosine-based activation motifs/ITAMs of the TCR zeta subunit and thereby negatively regulates TCR-mediated signaling pathway (By similarity).
May act at junctions between the membrane and the cytoskeleton
Catalytic activity
- H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 820 | substrate | ||||
Sequence: D | ||||||
Active site | 852 | Phosphocysteine intermediate | ||||
Sequence: C | ||||||
Binding site | 852-858 | substrate | ||||
Sequence: CSAGIGR | ||||||
Binding site | 896 | substrate | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytoplasmic side of plasma membrane | |
Cellular Component | cytoskeleton | |
Cellular Component | cytosol | |
Cellular Component | nucleoplasm | |
Molecular Function | cytoskeletal protein binding | |
Molecular Function | glutamate receptor binding | |
Molecular Function | non-membrane spanning protein tyrosine phosphatase activity | |
Molecular Function | protein tyrosine phosphatase activity | |
Biological Process | protein dephosphorylation |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTyrosine-protein phosphatase non-receptor type 4
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionP29074
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Keywords
- Cellular component
Disease & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | VAR_061033 | 924 | in dbSNP:rs3189128 | |||
Sequence: T → S |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 814 variants from UniProt as well as other sources including ClinVar and dbSNP.
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000219434 | 1-926 | Tyrosine-protein phosphatase non-receptor type 4 | |||
Sequence: MTSRFRLPAGRTYNVRASELARDRQHTEVVCNILLLDNTVQAFKVNKHDQGQVLLDVVFKHLDLTEQDYFGLQLADDSTDNPRWLDPNKPIRKQLKRGSPYSLNFRVKFFVSDPNKLQEEYTRYQYFLQIKQDILTGRLPCPSNTAALLASFAVQSELGDYDQSENLSGYLSDYSFIPNQPQDFEKEIAKLHQQHIGLSPAEAEFNYLNTARTLELYGVEFHYARDQSNNEIMIGVMSGGILIYKNRVRMNTFPWLKIVKISFKCKQFFIQLRKELHESRETLLGFNMVNYRACKNLWKACVEHHTFFRLDRPLPPQKNFFAHYFTLGSKFRYCGRTEVQSVQYGKEKANKDRVFARSPSKPLARKLMDWEVVSRNSISDDRLETQSLPSRSPPGTPNHRNSTFTQEGTRLRPSSVGHLVDHMVHTSPSEVFVNQRSPSSTQANSIVLESSPSQETPGDGKPPALPPKQSKKNSWNQIHYSHSQQDLESHINETFDIPSSPEKPTPNGGIPHDNLVLIRMKPDENGRFGFNVKGGYDQKMPVIVSRVAPGTPADLCVPRLNEGDQVVLINGRDIAEHTHDQVVLFIKASCERHSGELMLLVRPNAVYDVVEEKLENEPDFQYIPEKAPLDSVHQDDHSLRESMIQLAEGLITGTVLTQFDQLYRKKPGMTMSCAKLPQNISKNRYRDISPYDATRVILKGNEDYINANYINMEIPSSSIINQYIACQGPLPHTCTDFWQMTWEQGSSMVVMLTTQVERGRVKCHQYWPEPTGSSSYGCYQVTCHSEEGNTAYIFRKMTLFNQEKNESRPLTQIQYIAWPDHGVPDDSSDFLDFVCHVRNKRAGKEEPVVVHCSAGIGRTGVLITMETAMCLIECNQPVYPLDIVRTMRDQRAMMIQTPSQYRFVCEAILKVYEEGFVKPLTTSTNK | ||||||
Modified residue | 474 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Highly phosphorylated on serine and threonine residues but not on tyrosines.
Cleaved and activated by calpain I/CAPN1.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Interaction
Subunit
Interacts with MAPK12 (via C-terminus); this interaction abolishes PTPN4 catalytic autoinhibition and thus activates the phosphatase activity.
(Microbial infection) Interacts with attenuated rabies virus protein G; this interaction is required for virally-induced apoptosis.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P29074 | CASP6 P55212 | 3 | EBI-710431, EBI-718729 | |
BINARY | P29074 | CCK P06307 | 3 | EBI-710431, EBI-6624398 | |
BINARY | P29074 | CRK P46108 | 3 | EBI-710431, EBI-886 | |
BINARY | P29074 | KAT5 Q92993 | 2 | EBI-710431, EBI-399080 | |
BINARY | P29074 | LAMP2 P13473-2 | 3 | EBI-710431, EBI-21591415 | |
BINARY | P29074 | MAPK12 P53778 | 2 | EBI-710431, EBI-602406 | |
BINARY | P29074 | NCK1 P16333 | 3 | EBI-710431, EBI-389883 | |
BINARY | P29074 | PRPF40A O75400-2 | 3 | EBI-710431, EBI-5280197 |
Protein-protein interaction databases
Chemistry
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 29-312 | FERM | ||||
Sequence: VVCNILLLDNTVQAFKVNKHDQGQVLLDVVFKHLDLTEQDYFGLQLADDSTDNPRWLDPNKPIRKQLKRGSPYSLNFRVKFFVSDPNKLQEEYTRYQYFLQIKQDILTGRLPCPSNTAALLASFAVQSELGDYDQSENLSGYLSDYSFIPNQPQDFEKEIAKLHQQHIGLSPAEAEFNYLNTARTLELYGVEFHYARDQSNNEIMIGVMSGGILIYKNRVRMNTFPWLKIVKISFKCKQFFIQLRKELHESRETLLGFNMVNYRACKNLWKACVEHHTFFRLDR | ||||||
Region | 380-412 | Disordered | ||||
Sequence: DDRLETQSLPSRSPPGTPNHRNSTFTQEGTRLR | ||||||
Compositional bias | 430-457 | Polar residues | ||||
Sequence: EVFVNQRSPSSTQANSIVLESSPSQETP | ||||||
Region | 430-475 | Disordered | ||||
Sequence: EVFVNQRSPSSTQANSIVLESSPSQETPGDGKPPALPPKQSKKNSW | ||||||
Domain | 517-589 | PDZ | ||||
Sequence: LIRMKPDENGRFGFNVKGGYDQKMPVIVSRVAPGTPADLCVPRLNEGDQVVLINGRDIAEHTHDQVVLFIKAS | ||||||
Domain | 655-911 | Tyrosine-protein phosphatase | ||||
Sequence: VLTQFDQLYRKKPGMTMSCAKLPQNISKNRYRDISPYDATRVILKGNEDYINANYINMEIPSSSIINQYIACQGPLPHTCTDFWQMTWEQGSSMVVMLTTQVERGRVKCHQYWPEPTGSSSYGCYQVTCHSEEGNTAYIFRKMTLFNQEKNESRPLTQIQYIAWPDHGVPDDSSDFLDFVCHVRNKRAGKEEPVVVHCSAGIGRTGVLITMETAMCLIECNQPVYPLDIVRTMRDQRAMMIQTPSQYRFVCEAILKV |
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length926
- Mass (Da)105,911
- Last updated1992-12-01 v1
- Checksum4DAC6A87A675CFB0
Computationally mapped potential isoform sequences
There are 6 potential isoforms mapped to this entry
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 131 | in Ref. 2; BAG37355 | ||||
Sequence: K → E | ||||||
Compositional bias | 430-457 | Polar residues | ||||
Sequence: EVFVNQRSPSSTQANSIVLESSPSQETP | ||||||
Sequence conflict | 772 | in Ref. 4; no nucleotide entry | ||||
Sequence: G → K | ||||||
Sequence conflict | 863 | in Ref. 2; BAG37355 | ||||
Sequence: I → V |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M68941 EMBL· GenBank· DDBJ | AAA36530.1 EMBL· GenBank· DDBJ | mRNA | ||
AK314836 EMBL· GenBank· DDBJ | BAG37355.1 EMBL· GenBank· DDBJ | mRNA | ||
BC010674 EMBL· GenBank· DDBJ | AAH10674.1 EMBL· GenBank· DDBJ | mRNA |