P29056 · TF3B_YEAST
- ProteinTranscription factor IIIB 70 kDa subunit
- GeneBRF1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids596 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
General activator of RNA polymerase III transcription. Interacts with TBP. Binds to Pol III subunit C34 and to the TAU135 component of TFIIIC.
Miscellaneous
Present with 4330 molecules/cell in log phase SD medium.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleoplasm | |
Cellular Component | nucleus | |
Cellular Component | transcription factor TFIIIB complex | |
Cellular Component | transcription preinitiation complex | |
Molecular Function | DNA binding | |
Molecular Function | metal ion binding | |
Molecular Function | RNA polymerase III core binding | |
Molecular Function | RNA polymerase III general transcription initiation factor activity | |
Molecular Function | RNA polymerase III type 3 promoter sequence-specific DNA binding | |
Molecular Function | TBP-class protein binding | |
Molecular Function | TFIIIC-class transcription factor complex binding | |
Biological Process | DNA-templated transcription initiation | |
Biological Process | DNA-templated transcription open complex formation | |
Biological Process | regulation of transcription by RNA polymerase III | |
Biological Process | RNA polymerase III preinitiation complex assembly | |
Biological Process | transcription by RNA polymerase III |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTranscription factor IIIB 70 kDa subunit
- Short namesTFIIIB
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP29056
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000119346 | 1-596 | Transcription factor IIIB 70 kDa subunit | |||
Sequence: MPVCKNCHGTEFERDLSNANNDLVCKACGVVSEDNPIVSEVTFGETSAGAAVVQGSFIGAGQSHAAFGGSSALESREATLNNARRKLRAVSYALHIPEYITDAAFQWYKLALANNFVQGRRSQNVIASCLYVACRKEKTHHMLIDFSSRLQVSVYSIGATFLKMVKKLHITELPLADPSLFIQHFAEKLDLADKKIKVVKDAVKLAQRMSKDWMFEGRRPAGIAGACILLACRMNNLRRTHTEIVAVSHVAEETLQQRLNEFKNTKAAKLSVQKFRENDVEDGEARPPSFVKNRKKERKIKDSLDKEEMFQTSEEALNKNPILTQVLGEQELSSKEVLFYLKQFSERRARVVERIKATNGIDGENIYHEGSENETRKRKLSEVSIQNEHVEGEDKETEGTEEKVKKVKTKTSEEKKENESGHFQDAIDGYSLETDPYCPRNLHLLPTTDTYLSKVSDDPDNLEDVDDEELNAHLLNEEASKLKERIWIGLNADFLLEQESKRLKQEADIATGNTSVKKKRTRRRNNTRSDEPTKTVDAAAAIGLMSDLQDKSGLHAALKAAEESGDFTTADSVKNMLQKASFSKKINYDAIDGLFR | ||||||
Modified residue | 381 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 384 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
TFIIIB comprises the TATA-binding protein (TBP), the B-related factor (BRF) and the B' component (TFC5).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P29056 | SPT15 P13393 | 3 | EBI-19142, EBI-19129 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for zinc finger, repeat, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 1-33 | TFIIB-type | ||||
Sequence: MPVCKNCHGTEFERDLSNANNDLVCKACGVVSE | ||||||
Repeat | 90-166 | 1 | ||||
Sequence: VSYALHIPEYITDAAFQWYKLALANNFVQGRRSQNVIASCLYVACRKEKTHHMLIDFSSRLQVSVYSIGATFLKMVK | ||||||
Repeat | 185-264 | 2 | ||||
Sequence: FAEKLDLADKKIKVVKDAVKLAQRMSKDWMFEGRRPAGIAGACILLACRMNNLRRTHTEIVAVSHVAEETLQQRLNEFKN | ||||||
Region | 363-421 | Disordered | ||||
Sequence: GENIYHEGSENETRKRKLSEVSIQNEHVEGEDKETEGTEEKVKKVKTKTSEEKKENESG | ||||||
Compositional bias | 367-421 | Basic and acidic residues | ||||
Sequence: YHEGSENETRKRKLSEVSIQNEHVEGEDKETEGTEEKVKKVKTKTSEEKKENESG | ||||||
Region | 509-534 | Disordered | ||||
Sequence: IATGNTSVKKKRTRRRNNTRSDEPTK |
Sequence similarities
Belongs to the TFIIB family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length596
- Mass (Da)66,906
- Last updated1992-12-01 v1
- Checksum489207643A66B796
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 367-421 | Basic and acidic residues | ||||
Sequence: YHEGSENETRKRKLSEVSIQNEHVEGEDKETEGTEEKVKKVKTKTSEEKKENESG |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M91073 EMBL· GenBank· DDBJ | AAB04945.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
L00630 EMBL· GenBank· DDBJ | AAA35148.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Y07703 EMBL· GenBank· DDBJ | CAA68968.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z73031 EMBL· GenBank· DDBJ | CAA97275.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006941 EMBL· GenBank· DDBJ | DAA08337.1 EMBL· GenBank· DDBJ | Genomic DNA |