P28847 · RIR2_EHV1B
- ProteinRibonucleoside-diphosphate reductase small subunit
- GeneRIR2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids321 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Catalytic activity
- [thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate
RHEA-COMP:10700 CHEBI:50058 Position: n/n+3+ CHEBI:73316 + CHEBI:15377 = RHEA-COMP:10698 CHEBI:29950 Position: nCHEBI:29950 Position: n+3+ CHEBI:57930
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 78 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 108 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 108 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 111 | Fe cation 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 115 | |||||
Sequence: Y | ||||||
Binding site | 171 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 205 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 208 | Fe cation 2 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell membrane | |
Cellular Component | membrane | |
Molecular Function | metal ion binding | |
Molecular Function | ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor | |
Biological Process | deoxyribonucleotide biosynthetic process | |
Biological Process | DNA replication | |
Biological Process | release from viral latency |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibonucleoside-diphosphate reductase small subunit
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageViruses > Duplodnaviria > Heunggongvirae > Peploviricota > Herviviricetes > Herpesvirales > Orthoherpesviridae > Alphaherpesvirinae > Varicellovirus > Varicellovirus equidalpha1 > Equid alphaherpesvirus 1
- Virus hosts
Accessions
- Primary accessionP28847
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Host membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 165-185 | Helical | ||||
Sequence: ILMILIEGLFFASSFASIAYL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000190508 | 1-321 | Ribonucleoside-diphosphate reductase small subunit | |||
Sequence: MSIENSKEAALTAELSLAGAFFYTPECPDIEHLRSLSVANRWLDTDLPISDDLKDVAKLTPAEREFYRFLFAFLSAADDLVNLNLGDLSALFTQKDILHYYIEQESIEVTHSRVYSAIQLMLFGNDAAARARYVASIIGDAAIGRKVAWLQAKVRECGSVAEKYILMILIEGLFFASSFASIAYLRTHNLFVVTCQSNDLISRDEAIHTRASCCIYNNYLGGFEKPEPKRIYELFSEAVNIECEFLLSHAPQYSHLLDIGAIISYVRYSADRLLGEIGLSPLFNAPKPSPSFPLAFMTVEKHTNFFERRSTAYSGTLINDL |
Interaction
Subunit
Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.
Sequence
- Sequence statusComplete
- Length321
- Mass (Da)36,017
- Last updated1992-12-01 v1
- Checksum81E1FF57B12B84A4
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY665713 EMBL· GenBank· DDBJ | AAT67277.1 EMBL· GenBank· DDBJ | Genomic DNA |