P27922 · SC6A3_BOVIN
- ProteinSodium-dependent dopamine transporter
- GeneSLC6A3
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids693 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Mediates sodium- and chloride-dependent transport of dopamine (PubMed:1722321).
Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline) (By similarity).
Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (By similarity).
Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline) (By similarity).
Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling (By similarity).
Miscellaneous
This protein is the target of psychomotor stimulants such as amphetamines or cocaine.
Catalytic activity
- chloride(out) + dopamine(out) + Na+(out) = chloride(in) + dopamine(in) + Na+(in)chloride (out)CHEBI:17996
+ dopamine (out)CHEBI:59905+ Na+ (out)CHEBI:29101= chloride (in)CHEBI:17996+ dopamine (in)CHEBI:59905+ Na+ (in)CHEBI:29101 - (R)-noradrenaline(out) + chloride(out) + Na+(out) = (R)-noradrenaline(in) + chloride(in) + Na+(in)
- chloride(out) + dopamine(out) + 2 Na+(out) = chloride(in) + dopamine(in) + 2 Na+(in)
Activity regulation
Inhibited by GBR 12909 dihydrochloride, amphetamine and cocaine.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
31.5 μM | dopamine |
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 75 | Na+ 1 (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 77 | Na+ 2 (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 78 | Na+ 1 (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 82 | Na+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Site | 105 | Contributes to high-affinity binding to cocaine | ||||
Sequence: F | ||||||
Binding site | 318 | Na+ 2 (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 350 | Na+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 415 | Na+ 1 (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 418 | Na+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 419 | Na+ 1 (UniProtKB | ChEBI) | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | axon | |
Cellular Component | neuron projection | |
Cellular Component | neuronal cell body membrane | |
Cellular Component | plasma membrane | |
Cellular Component | presynaptic membrane | |
Molecular Function | dopamine:sodium symporter activity | |
Molecular Function | metal ion binding | |
Molecular Function | norepinephrine:sodium symporter activity | |
Biological Process | amino acid transport | |
Biological Process | dopamine transport | |
Biological Process | dopamine uptake involved in synaptic transmission | |
Biological Process | hyaloid vascular plexus regression | |
Biological Process | norepinephrine transport | |
Biological Process | sodium ion transmembrane transport |
Keywords
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameSodium-dependent dopamine transporter
- Short namesDA transporter; DAT
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionP27922
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Note: Localizes to neurite tips in neuronal cells (By similarity).
Colocalizes with SEPTIN4 at axon terminals, especially at the varicosities (By similarity).
Colocalizes with SEPTIN4 at axon terminals, especially at the varicosities (By similarity).
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-68 | Cytoplasmic | ||||
Sequence: MSEGRCSVAHMSSVVAPAKEANAMGPKAVELVLVKEQNGVQLTNSTLLNPPQSPTEAQDRETWSKKAD | ||||||
Transmembrane | 69-89 | Helical; Name=1 | ||||
Sequence: FLLSVIGFAVDLANVWRFPYL | ||||||
Transmembrane | 97-116 | Helical; Name=2 | ||||
Sequence: AFLVPYLFFMVVAGVPLFYM | ||||||
Transmembrane | 140-160 | Helical; Name=3 | ||||
Sequence: GVGYTAILISLYIGFFYNVII | ||||||
Topological domain | 161-234 | Extracellular | ||||
Sequence: AWALHYLLSSFTTELPWTHCNHSWNSPRCSDARAPNASSGPNGTSRTTPAAEYFERGVLHLHESQGIDDLGPPR | ||||||
Transmembrane | 235-253 | Helical; Name=4 | ||||
Sequence: WQLTSCLVLVIVLLYFSLW | ||||||
Transmembrane | 262-279 | Helical; Name=5 | ||||
Sequence: VVWITATMPYVVLFALLL | ||||||
Transmembrane | 315-332 | Helical; Name=6 | ||||
Sequence: ICFSLGVGLGVLIAFSSY | ||||||
Transmembrane | 344-365 | Helical; Name=7 | ||||
Sequence: IITTSVNSLTSFSSGFVVFSFL | ||||||
Transmembrane | 398-417 | Helical; Name=8 | ||||
Sequence: LPLSSVWAVVFFVMLLTLGI | ||||||
Transmembrane | 444-462 | Helical; Name=9 | ||||
Sequence: LFTLLVVLATFLLSLFCVT | ||||||
Transmembrane | 478-498 | Helical; Name=10 | ||||
Sequence: GTSILFGVLMEVIGVAWFYGV | ||||||
Transmembrane | 519-538 | Helical; Name=11 | ||||
Sequence: LCWKFVSPCFLLFVVVVSIA | ||||||
Transmembrane | 557-575 | Helical; Name=12 | ||||
Sequence: LGWAIAASSMSVVPIYAAY | ||||||
Topological domain | 576-693 | Cytoplasmic | ||||
Sequence: KLCSLPGSSREKLAYAITPETEHGRVDSGGGAPVHAPPLARGVGRWRKRKSCWVPSRGPGRGGPPTPSPRLAGHTRAFPWTGAPPVPRELTPPSTCRCVPPLVCAHPAVESTGLCSVY |
Keywords
- Cellular component
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for chain, disulfide bond, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000214750 | 1-693 | Sodium-dependent dopamine transporter | |||
Sequence: MSEGRCSVAHMSSVVAPAKEANAMGPKAVELVLVKEQNGVQLTNSTLLNPPQSPTEAQDRETWSKKADFLLSVIGFAVDLANVWRFPYLCYKNGGGAFLVPYLFFMVVAGVPLFYMELALGQFNREGAAGVWKICPILRGVGYTAILISLYIGFFYNVIIAWALHYLLSSFTTELPWTHCNHSWNSPRCSDARAPNASSGPNGTSRTTPAAEYFERGVLHLHESQGIDDLGPPRWQLTSCLVLVIVLLYFSLWKGVKTSGKVVWITATMPYVVLFALLLRGITLPGAVDAIRAYLSVDFHRLCEASVWIDAAIQICFSLGVGLGVLIAFSSYNKFTNNCYRDAIITTSVNSLTSFSSGFVVFSFLGYMAQKHSVPIGDVAKDGPGLIFIIYPEALATLPLSSVWAVVFFVMLLTLGIDSAMGGMESVITGLADEFQLLHRHRELFTLLVVLATFLLSLFCVTNGGIYVFTLLDHFAAGTSILFGVLMEVIGVAWFYGVWQFSDDIKQMTGRRPSLYWRLCWKFVSPCFLLFVVVVSIATFRPPHYGAYVFPEWATALGWAIAASSMSVVPIYAAYKLCSLPGSSREKLAYAITPETEHGRVDSGGGAPVHAPPLARGVGRWRKRKSCWVPSRGPGRGGPPTPSPRLAGHTRAFPWTGAPPVPRELTPPSTCRCVPPLVCAHPAVESTGLCSVY | ||||||
Disulfide bond | 180↔189 | |||||
Sequence: CNHSWNSPRC | ||||||
Glycosylation | 181 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 196 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 202 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 303 | Interchain | ||||
Sequence: C |
Keywords
- PTM
PTM databases
Expression
Tissue specificity
Expressed in the neurons of the substantia nigra of the brain.
Interaction
Subunit
Homooligomer; disulfide-linked (By similarity).
Interacts with PRKCABP and TGFB1I1 (By similarity).
Interacts (via N-terminus) with SYNGR3 (via N-terminus) (By similarity).
Interacts with SLC18A2 (By similarity).
Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location (By similarity).
Interacts with alpha-synuclein/SNCA (By similarity).
Interacts with SEPTIN4 (By similarity).
Interacts with PRKCABP and TGFB1I1 (By similarity).
Interacts (via N-terminus) with SYNGR3 (via N-terminus) (By similarity).
Interacts with SLC18A2 (By similarity).
Interacts with TOR1A (ATP-bound); TOR1A regulates SLC6A3 subcellular location (By similarity).
Interacts with alpha-synuclein/SNCA (By similarity).
Interacts with SEPTIN4 (By similarity).
Protein-protein interaction databases
Chemistry
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 558-587 | Interaction with TGFB1I1 | ||||
Sequence: GWAIAASSMSVVPIYAAYKLCSLPGSSREK |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length693
- Mass (Da)75,691
- Last updated1992-08-01 v1
- Checksum0023A6F6D0698660
Keywords
- Technical term