P27743 · ACVS_AMYLA
- ProteinN-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase
- GenepcbAB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids3649 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Each of the constituent amino acids of the tripeptide acv are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates.
Catalytic activity
- 3 ATP + H2O + L-2-aminoadipate + L-cysteine + L-valine = 3 AMP + 3 diphosphate + 3 H+ + N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine
Cofactor
Note: Binds 3 phosphopantetheines covalently.
Pathway
Antibiotic biosynthesis; penicillin G biosynthesis; penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine: step 1/3.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 3502 | For thioesterase activity | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase activity | |
Molecular Function | phosphopantetheine binding | |
Biological Process | amino acid activation for nonribosomal peptide biosynthetic process | |
Biological Process | antibiotic biosynthetic process | |
Biological Process | lipid biosynthetic process | |
Biological Process | secondary metabolite biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameN-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Pseudonocardiales > Pseudonocardiaceae > Amycolatopsis
Accessions
- Primary accessionP27743
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000193059 | 1-3649 | N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase | |||
Sequence: MTSARHLKSAADWCARIDAIAGQRCDLEMLLKDEWRHRVAVRDSDTAVRATQEKELTISGQDYTALKQALGAMPLEAFALATLHSVLHAYGHGHQTVVAFLRDGKVLPVVVDHLEQAGLTCAEAAEQLEDAVAREDMYLPPEELLQRGLFDALLVLADGHLGFTELPPAPLVTIVRDDPAAGCLHWRIAYAGEFFEDKIIAGVLDVAREVLGQFIGRPEQLVADIDLVSAEQELQLHQWNGTDGEFDEDKRLNELFEDVVRRAPDREAVVCGDVRLTYREVNERANQFAHWLIQGPVRVRPGALIGLYLDKSDLGVVATFGIWKSGAAYVPIDPAYPAERIRFLVGDTGLSGIVTNRRHAERLREVLGDEHASVHVIEVEAVVAGPHPEQARENPGLALSSRDRAYVTYTSGTTGVPKGVPKYHYSVVNSITDLSERYDMRRPGTERVALFASYVFEPHLRQTLIALINEQTLVIVPDDVRLDPDLFPEYIERHGVTYLNATGSVLQHFDLRRCASLKRLLLVGEELTASGLRQLREKFAGRVVNEYAFTEAAFVTAVKEFGPGVTERRDRSIGRPLRNVKWYVLSQGLKQLPIGAIGELYIGGCGVAPGYLNRDDLTAERFTANPFQTEEEKARGRNGRLYRTGDLARVLLNGEVEFMGRADFQLKLNGVRVEPGEIEAQATEFPGVKKCVVVAKENATGDRHLVGYYLVEDGAEVAEADLIAFLEQRLIRIMVPARMVRLTSIPVNVNGKVDWRALPDVSLHPAPANAMNGALLAIDGSNAPLLAITEQLRAIWSEVLGVPQNRIGERDDFFRLGGQSISCILLIARVRQRLSLSLGVEDVFALRTLDALAGHLESQGHAEPEVVAEEVTTGSEPVRVLANGLQQGLLYHHLKTAGGDDAYVVQSVHRYHAPIRPELMKDAWQAARQTYPALRLRFDWAEEPVQIVDNDDKPFDWRFVDLSATADDAEQEARVRELQERDRTEPYDLAGGRLFRVYLIKQREDLFSLIFSCHHIILDGWSLPVLHDEVHRNYLALRAGQPIESDVDNAYVAAQRYWEAHRNDHAAYWVEQLGRIDERGDFAGLLNEKSRYRVSLGDYDHVQRHRTRKLYLGADLTGALKAGCAADQVTLHSVLQFVWHKVLHAIGGGNTTVVGTIVSGRNLPVDGIENSAGLFINTLPLIVDHDQQAGQNVAEAVRDIQAAVNTMNSKSIVELGRLQSGEMKRRLFDTLLVLENYPRLLDEEEELAHQEALRFEKAYDADKVDYPIAVVAREEGDELTVTLWYAGELFDEDTIDTLLDVARTLFRQVTEDIARPVRELDLISPSMRARFDSWNETAEEFPADKTLHAVFEEMAERWPDEIAVVYRENRLTYRELNERANRLAHYLRSVVELRPDDLVALVLDKSELMITAIIAAWKTGAAYVPIDSGYPDDRISFMLSDTAARVVVTNEIHSDRLRSLAETGTPVLEIELLHLDDQPAVNPVTETTSTDLAYAIYTSGTTGKPKAVLVEHRGVVNLQVSLAKLFGLDKAHRDEALLSFSNYIFDHFVEQMTDALLNGQKLVVLDDSMRTDPGRLCRYMNDEQVTYLSGTPSVLSLYDYSSATSLTRIDAIGEDFTEPVFAKIRGTFPGLIINGYGPTEISITSHKRPYPPDVHRVNKSIGFPVANTKCHVLNKAMKPVPVGGIGELYIGGIGVTRGYLNREDLTADRFVENPFQTAEERRLGENGRLYKTGDLVRWLPNGEVEYLGRTDLQVKIRGQRVELGEVEAALSSYPGVVRSLVVAREHAVGQKYLVGFYVGEQEFDEQDLKQWMRKKLPESVVPARVLRITDIPVTPSGKLDARRLPETDFGAGEGAEYVAPVSEFELKLCGIWAQVLEIAPDRIGVHDDFFALGGDSIRAMALAQAITTGFGQGLGVATVLQHTTLAAQAEHIQAAALEHTAWTPPPTAVEHPPVSLAQERLLFIDDFEGGTAAYNIPFVLRLPAHTRAALPGALGTLVRRHPALRTLLKTDDQGVRRQYPIPADDVRLEVPSTTVDSRAELDEVLTERAGYVFRLHEELPIRAEAFDHGDEIYLSVVVHHSCFDGWSWDIFRRELAALLDGVPEADLGALRGTYGEFAVWQRQYLTGKRLAALTEFWTGALGGFETIALPLDHPRPPRFDYRGRELEFELDERTTEALRELARTARVSLYSVLLGAWCLMLNMYTGQHDLVVGTPSANRGRPEFDRAVGFFANLLALRVRVDPAATLPAYVRSVGEAVVAAQVHGELPFEQLVKELKVEKDPSRHPILQLNFTLQNVSDHTSALTGYQPDSGGWTTTKFDLSATMTETATGLAGNLTYAASLFDDTSASGFIATFKHVLAEFASAAAQTPIAQLTALDEPGQAALPDATRRARRPGGPGRCTRLFEEVAATWPDRVAVVHGDVRLTYRELNERANRLAHHLRSVAEPRADELIALVLDKSELTLVAILAVWKAGAAYMPIDPSYPDDRIAFMLSDTGAKLVLAGEAHGSRVRGLTSGDVLDLEQLDLTGEPAENPVTETTSTELAYAIYTSGTTGKPKAVLVSHGSVDSFRAQLSGRYFGSPDESAEAVLFLANYVFDFSVEQLALSVLGGHKLLVPPPSAADDPAFYELANREGLSYLSGTPTQVERFDLARLSHLRCVLVAGEAFQPQHFEKMRGEFAGPILNAYGTTETTVYNTVHRFEPGDAYRNTLGAPLGNTRLYVLGDGMKLLPTGAVGELYLAGDCVTEGYLHRPELTRERFLPNPFAAESGRFPMIYRTGDVVRRGPDGELQYLGRNDAQVKINGLRIEPGEVEAALAGCSGVRQCAVVAGADPQAPERKRLVGYYLPEPGAAVDEADLFAALRAQLMPSMVPSLLVRLDRPLPMTITGKLDVDALPSADFSPKRAAYAAPRDRVEARLCHLWSAQLPGGTVGIDDDFFRCGGDSISALHLASQVQREIERKVSVKYLFDHPTVRSFVDNVLSGLAESSGDDEPEQGRLTGECPMLPIQEWFFAKPLADRHRWNHNFAIRTPPLDPGELRTALDRLVEHHDAFRLRFPESGGEVYAEDAAPITLHELDVRGLADADLRQRLVDWQRTFDLANGPTACAAYLHGFDDGTARVWFALHHLVVDTVSWHILAQDLEILYNGGDLGAKTGSYRQWAQAVRDYTPAEGEREFWAETTRDMESAELLAQTEGTTRRREEFALTAPDTRTLLAESPWAYDTEVNDLLLTATGFALRSITRQATNHLTVEGHGRELFEGAPDVRDTVGWFTTMHPFAVEVDPGDLGRSVLATRANRRRVPHHGIGYGALFGGEAPLPAVSFNYLGRLGEGDGQPTEAWQLDPALSGSHTVDGNRLANRSSIDVTMSCTGGRLVAVVDSLLGEAATRLFASELKVWLERLVSHTATVARNEPAREATTELFDPYILVNEDAERTLFVLPPGEGGAESYLSNLARQLPDLRLVLFNNVHLHTPMGSFEELGRYYVEHIRRLQPSGPYHLLGWSFGGVLSLEISRQLARAGERIDDLLLIDPYFGMRQASANIGLPGVEDILDPINYHYRPDEADLARLAGRLGNLVLFKAGEPNDVVNGPHQPRLFEYYHGTRFNHLDLLLPAAAIEVCDLAGETHHSWVRNEKLVRLMCERISTSLGSD | ||||||
Modified residue | 820 | O-(pantetheine 4'-phosphoryl)serine | ||||
Sequence: S | ||||||
Modified residue | 1896 | O-(pantetheine 4'-phosphoryl)serine | ||||
Sequence: S | ||||||
Modified residue | 2944 | O-(pantetheine 4'-phosphoryl)serine | ||||
Sequence: S |
Keywords
- PTM
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 401-861 | Domain 1 (adipate-activating) | ||||
Sequence: SRDRAYVTYTSGTTGVPKGVPKYHYSVVNSITDLSERYDMRRPGTERVALFASYVFEPHLRQTLIALINEQTLVIVPDDVRLDPDLFPEYIERHGVTYLNATGSVLQHFDLRRCASLKRLLLVGEELTASGLRQLREKFAGRVVNEYAFTEAAFVTAVKEFGPGVTERRDRSIGRPLRNVKWYVLSQGLKQLPIGAIGELYIGGCGVAPGYLNRDDLTAERFTANPFQTEEEKARGRNGRLYRTGDLARVLLNGEVEFMGRADFQLKLNGVRVEPGEIEAQATEFPGVKKCVVVAKENATGDRHLVGYYLVEDGAEVAEADLIAFLEQRLIRIMVPARMVRLTSIPVNVNGKVDWRALPDVSLHPAPANAMNGALLAIDGSNAPLLAITEQLRAIWSEVLGVPQNRIGERDDFFRLGGQSISCILLIARVRQRLSLSLGVEDVFALRTLDALAGHLESQGH | ||||||
Domain | 783-860 | Carrier 1 | ||||
Sequence: APLLAITEQLRAIWSEVLGVPQNRIGERDDFFRLGGQSISCILLIARVRQRLSLSLGVEDVFALRTLDALAGHLESQG | ||||||
Region | 1014-1937 | Domain 2 (cysteine-activating) | ||||
Sequence: HHIILDGWSLPVLHDEVHRNYLALRAGQPIESDVDNAYVAAQRYWEAHRNDHAAYWVEQLGRIDERGDFAGLLNEKSRYRVSLGDYDHVQRHRTRKLYLGADLTGALKAGCAADQVTLHSVLQFVWHKVLHAIGGGNTTVVGTIVSGRNLPVDGIENSAGLFINTLPLIVDHDQQAGQNVAEAVRDIQAAVNTMNSKSIVELGRLQSGEMKRRLFDTLLVLENYPRLLDEEEELAHQEALRFEKAYDADKVDYPIAVVAREEGDELTVTLWYAGELFDEDTIDTLLDVARTLFRQVTEDIARPVRELDLISPSMRARFDSWNETAEEFPADKTLHAVFEEMAERWPDEIAVVYRENRLTYRELNERANRLAHYLRSVVELRPDDLVALVLDKSELMITAIIAAWKTGAAYVPIDSGYPDDRISFMLSDTAARVVVTNEIHSDRLRSLAETGTPVLEIELLHLDDQPAVNPVTETTSTDLAYAIYTSGTTGKPKAVLVEHRGVVNLQVSLAKLFGLDKAHRDEALLSFSNYIFDHFVEQMTDALLNGQKLVVLDDSMRTDPGRLCRYMNDEQVTYLSGTPSVLSLYDYSSATSLTRIDAIGEDFTEPVFAKIRGTFPGLIINGYGPTEISITSHKRPYPPDVHRVNKSIGFPVANTKCHVLNKAMKPVPVGGIGELYIGGIGVTRGYLNREDLTADRFVENPFQTAEERRLGENGRLYKTGDLVRWLPNGEVEYLGRTDLQVKIRGQRVELGEVEAALSSYPGVVRSLVVAREHAVGQKYLVGFYVGEQEFDEQDLKQWMRKKLPESVVPARVLRITDIPVTPSGKLDARRLPETDFGAGEGAEYVAPVSEFELKLCGIWAQVLEIAPDRIGVHDDFFALGGDSIRAMALAQAITTGFGQGLGVATVLQHTTLAAQAEHIQAAAL | ||||||
Domain | 1859-1936 | Carrier 2 | ||||
Sequence: APVSEFELKLCGIWAQVLEIAPDRIGVHDDFFALGGDSIRAMALAQAITTGFGQGLGVATVLQHTTLAAQAEHIQAAA | ||||||
Region | 2079-2985 | Domain 3 (valine-activating) | ||||
Sequence: HHSCFDGWSWDIFRRELAALLDGVPEADLGALRGTYGEFAVWQRQYLTGKRLAALTEFWTGALGGFETIALPLDHPRPPRFDYRGRELEFELDERTTEALRELARTARVSLYSVLLGAWCLMLNMYTGQHDLVVGTPSANRGRPEFDRAVGFFANLLALRVRVDPAATLPAYVRSVGEAVVAAQVHGELPFEQLVKELKVEKDPSRHPILQLNFTLQNVSDHTSALTGYQPDSGGWTTTKFDLSATMTETATGLAGNLTYAASLFDDTSASGFIATFKHVLAEFASAAAQTPIAQLTALDEPGQAALPDATRRARRPGGPGRCTRLFEEVAATWPDRVAVVHGDVRLTYRELNERANRLAHHLRSVAEPRADELIALVLDKSELTLVAILAVWKAGAAYMPIDPSYPDDRIAFMLSDTGAKLVLAGEAHGSRVRGLTSGDVLDLEQLDLTGEPAENPVTETTSTELAYAIYTSGTTGKPKAVLVSHGSVDSFRAQLSGRYFGSPDESAEAVLFLANYVFDFSVEQLALSVLGGHKLLVPPPSAADDPAFYELANREGLSYLSGTPTQVERFDLARLSHLRCVLVAGEAFQPQHFEKMRGEFAGPILNAYGTTETTVYNTVHRFEPGDAYRNTLGAPLGNTRLYVLGDGMKLLPTGAVGELYLAGDCVTEGYLHRPELTRERFLPNPFAAESGRFPMIYRTGDVVRRGPDGELQYLGRNDAQVKINGLRIEPGEVEAALAGCSGVRQCAVVAGADPQAPERKRLVGYYLPEPGAAVDEADLFAALRAQLMPSMVPSLLVRLDRPLPMTITGKLDVDALPSADFSPKRAAYAAPRDRVEARLCHLWSAQLPGGTVGIDDDFFRCGGDSISALHLASQVQREIERKVSVKYLFDHPTVRSFVDNVLSGLA | ||||||
Domain | 2909-2984 | Carrier 3 | ||||
Sequence: APRDRVEARLCHLWSAQLPGGTVGIDDDFFRCGGDSISALHLASQVQREIERKVSVKYLFDHPTVRSFVDNVLSGL |
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length3,649
- Mass (Da)404,088
- Last updated1992-08-01 v1
- Checksum6FD095704F858E6B