P27548 · CD40L_MOUSE

  • Protein
    CD40 ligand
  • Gene
    Cd40lg
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Cytokine that acts as a ligand to CD40/TNFRSF5 (By similarity).
Costimulates T-cell proliferation and cytokine production (By similarity).
Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (By similarity).
Induces the activation of NF-kappa-B (By similarity).
Induces the activation of kinases MAPK8 and PAK2 in T-cells (By similarity).
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (PubMed:1374165).
Involved in immunoglobulin class switching (PubMed:1374165).

CD40 ligand, soluble form

Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

Features

Showing features for site.

126020406080100120140160180200220240260
TypeIDPosition(s)Description
Site111-112Cleavage

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcell body
Cellular Componentcell projection
Cellular Componentcell surface
Cellular Componentexternal side of plasma membrane
Cellular Componentextracellular space
Cellular ComponentGolgi apparatus
Molecular FunctionCD40 receptor binding
Molecular Functioncytokine activity
Molecular Functionintegrin binding
Molecular Functionprotein serine/threonine kinase activator activity
Molecular Functiontumor necrosis factor receptor binding
Biological ProcessB cell differentiation
Biological ProcessB cell proliferation
Biological ProcessCD40 signaling pathway
Biological Processdendritic cell differentiation
Biological Processinflammatory response
Biological Processintegrin-mediated signaling pathway
Biological Processisotype switching
Biological Processnegative regulation of apoptotic process
Biological Processplatelet activation
Biological Processpositive regulation of B cell proliferation
Biological Processpositive regulation of dendritic cell differentiation
Biological Processpositive regulation of endothelial cell apoptotic process
Biological Processpositive regulation of gene expression
Biological Processpositive regulation of immune response to tumor cell
Biological Processpositive regulation of interleukin-10 production
Biological Processpositive regulation of interleukin-12 production
Biological Processpositive regulation of interleukin-4 production
Biological Processpositive regulation of MHC class II biosynthetic process
Biological Processpositive regulation of NF-kappaB transcription factor activity
Biological Processpositive regulation of T cell proliferation
Biological Processpositive regulation of type II interferon production
Biological Processregulation of immunoglobulin production
Biological Processsignal transduction

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    CD40 ligand
  • Short names
    CD40-L
  • Alternative names
    • T-cell antigen Gp39
    • TNF-related activation protein (TRAP)
    • Tumor necrosis factor ligand superfamily member 5
  • Cleaved into 2 chains
  • CD Antigen Name
    • CD154

Gene names

    • Name
      Cd40lg
    • Synonyms
      Cd40l, Tnfsf5

Organism names

  • Taxonomic identifier
  • Strain
    • 129/Sv
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P27548

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Single-pass type II membrane protein
Cell surface

CD40 ligand, soluble form

Secreted
Note: Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-22Cytoplasmic
Transmembrane23-46Helical; Signal-anchor for type II membrane protein
Topological domain47-260Extracellular

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00000344901-260CD40 ligand, membrane form
ChainPRO_0000034491112-260CD40 ligand, soluble form
Disulfide bond177↔217
Glycosylation239N-linked (GlcNAc...) asparagine

Post-translational modification

The soluble form derives from the membrane form by proteolytic processing.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Specifically expressed on activated CD4+ T-lymphocytes.

Gene expression databases

Interaction

Subunit

Homotrimer (PubMed:8095800).
Interacts with CD28 (By similarity).
CD40 ligand, soluble form: Exists as either a monomer or a homotrimer (By similarity).
Forms a ternary complex between CD40 and integrins for CD40-CD40LG signaling (By similarity).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain121-260THD

Sequence similarities

Belongs to the tumor necrosis factor family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    260
  • Mass (Da)
    29,370
  • Last updated
    2001-08-14 v2
  • Checksum
    7E1AC117473672AD
MIETYSQPSPRSVATGLPASMKIFMYLLTVFLITQMIGSVLFAVYLHRRLDKVEEEVNLHEDFVFIKKLKRCNKGEGSLSLLNCEEMRRQFEDLVKDITLNKEEKKENSFEMQRGDEDPQIAAHVVSEANSNAASVLQWAKKGYYTMKSNLVMLENGKQLTVKREGLYYVYTQVTFCSNREPSSQRPFIVGLWLKPSSGSERILLKAANTHSSSQLCEQQSVHLGGVFELQAGASVFVNVTEASQVIHRVGFSSFGLLKL

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict198in Ref. 3; AAC13640

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X65453
EMBL· GenBank· DDBJ
CAA46448.2
EMBL· GenBank· DDBJ
mRNA
S71858
EMBL· GenBank· DDBJ
AAC13639.1
EMBL· GenBank· DDBJ
Genomic DNA
S71861
EMBL· GenBank· DDBJ
AAC13640.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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