P27398 · CAND_DROME
- ProteinCalpain-D
- Genesol
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1594 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Has a role in eye development.
Calcium-regulated non-lysosomal thiol-protease.
Miscellaneous
Although homology to other calpains is high within the protease domain, the lack of calcium-binding sites suggests that this protein is a protease that may not be activated by calcium ions.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 1079 | |||||
Sequence: C | ||||||
Active site | 1245 | |||||
Sequence: H | ||||||
Active site | 1265 | |||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | calcium-dependent cysteine-type endopeptidase activity | |
Molecular Function | metal ion binding | |
Biological Process | nervous system development | |
Biological Process | proteolysis | |
Biological Process | visual perception |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCalpain-D
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionP27398
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Disruption phenotype
Mutants cause specific cells to degenerate in the developing optic lobes, resulting in the absence of certain classes of columnar neurons.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000207733 | 1-1594 | Calpain-D | |||
Sequence: MGTISSVLQWSCTKCNTINPTESLKCFNCGTVRKVFPQQQQQQHRSSSITASWTADDALEQEQAEKGQERDKEKGRAAVARSEYKHVYKSLLRGCLKRPQRNSQNLPANCVDCEDTRKYIKSSIELYRHFSNPALNRRWVCHACGTDNSSVTWHCLICDTVSYLAPIYKDAIAADRGQDLAGSLGNRGELLAADHSHPHHHHHYLHQELEEQHQHQLHSQHLHKRHLKGRSASGSGSGPGSGSGLRRTQSLSTAIDKSASGRSCHICYANNQSKDIFNLPQIKPAPQLTGIPPVAACSNSRFAIANDTFCRRKQNNNNKNQNHKVVRESGAKRKYNFTITTLSRSAAKDAGHGQMKPLRQVVNLNLNLQQEPQQKSPANPQQLQRKTQREPAAVSMNPTQFTIPRNGVFIAVNEWSEPMASSSSVSSSSNHHHHHHSNSNSNSSGNSNIINNNSSSSSGSNKLYENECVALAQQQLRAAAAQAAQAAATAVAIASSPSAKAMAEPAPTATMPIYAQVNKQHKLKKKQQIASESQTNNNTGSGEIADAVSESLTAGLGTSTDGSGEASESESQVEEHSIYAKVWKGPRKATESKIMHDPGSSSRLSGAASAAAGTASAGAIAAAVGAAAASRHDNKTQLGNGSRSKMWICIKCSYAYNRLWLQTCEMCEAKAEQQQQQLQLQQQQQQQQQHHHHHLQQQQAEAPRDEPWTCKKCTLVNYSTAMACVVCGGSKLKSISSIEDMTLRKGEFWTCSHCTLKNSLHSPVCSACKSHRQPQLSMAMEAVRERPDGQSYEEQDAAAVGGGGGSAHQSGANEVKAPTALNLPLTSVALPMPMLQLPTSTAAGLRGSRSPSPRMQLLPSLQQQRNSSSSGAIPKRHSTGGSIVPRNISIAGLANYNLQQGQGVGSASVVSASGAGSGAGAVGASTSTKKWQCPACTYDNCAASVVCDICSSPRGLASAVLGEALGRKSVRVALTPADIRQESKLMENLRQLEETEALTKWQNIIQYCRDNSELFVDDSFPPAPKSLYYNPASGAGEGNPVVQWRRPHEINCDGGAYPPWAVFRTPLPSDICQGVLGNCWLLSALAVLAEREDLVKEVLVTKEICGQGAYQVRLCKDGKWTTVLVDDLLPCDKRGHLVYSQAKRKQLWVPLIEKAVAKIHGCYEALVSGRAIEGLATLTGAPCESIPLQASSLPMPSEDELDKDLIWAQLLSSRCVRFLMGASCGGGNMKVDEEEYQQKGLRPRHAYSVLDVKDIQGHRLLKLRNPWGHYSWRGDWSDDSSLWTDDLRDALMPHGASEGVFWISFEDVLNYFDCIDICKVRSGWNEVRLQGTLQPLCSISCVLLTVLEPTEAEFTLFQEGQRNSEKSQRSQLDLCVVIFRTRSPAAPEIGRLVEHSKRQVRGFVGCHKMLERDIYLLVCLAFNHWHTGIEDPHQYPQCILAIHSSKRLLVEQISPSPHLLADAIISLTLTKGQRHEGREGMTAYYLTKGWAGLVVMVENRHENKWIHVKCDCQESYNVVSTRGELKTVDSVPPLQRQVIIVLTQLEGSGGFSIAHRLTHRLANSRGLHDWGPPGATHCPPIENVHGLHAPRLIT | ||||||
Modified residue | 250 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Developmental stage
Present throughout development, with expression levels lower in larvae than other life stages.
Gene expression databases
Structure
Family & Domains
Features
Showing features for zinc finger, region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 1-35 | RanBP2-type 1 | ||||
Sequence: MGTISSVLQWSCTKCNTINPTESLKCFNCGTVRKV | ||||||
Zinc finger | 135-164 | RanBP2-type 2 | ||||
Sequence: LNRRWVCHACGTDNSSVTWHCLICDTVSYL | ||||||
Region | 210-256 | Disordered | ||||
Sequence: EEQHQHQLHSQHLHKRHLKGRSASGSGSGPGSGSGLRRTQSLSTAID | ||||||
Compositional bias | 234-256 | Polar residues | ||||
Sequence: GSGSGPGSGSGLRRTQSLSTAID | ||||||
Region | 371-400 | Disordered | ||||
Sequence: EPQQKSPANPQQLQRKTQREPAAVSMNPTQ | ||||||
Region | 420-459 | Disordered | ||||
Sequence: ASSSSVSSSSNHHHHHHSNSNSNSSGNSNIINNNSSSSSG | ||||||
Compositional bias | 439-459 | Polar residues | ||||
Sequence: SNSNSSGNSNIINNNSSSSSG | ||||||
Region | 524-543 | Disordered | ||||
Sequence: KKKQQIASESQTNNNTGSGE | ||||||
Compositional bias | 529-543 | Polar residues | ||||
Sequence: IASESQTNNNTGSGE | ||||||
Region | 554-606 | Disordered | ||||
Sequence: AGLGTSTDGSGEASESESQVEEHSIYAKVWKGPRKATESKIMHDPGSSSRLSG | ||||||
Compositional bias | 569-594 | Basic and acidic residues | ||||
Sequence: SESQVEEHSIYAKVWKGPRKATESKI | ||||||
Zinc finger | 643-673 | RanBP2-type 3 | ||||
Sequence: RSKMWICIKCSYAYNRLWLQTCEMCEAKAEQ | ||||||
Region | 684-703 | Disordered | ||||
Sequence: QQQQQQHHHHHLQQQQAEAP | ||||||
Zinc finger | 704-733 | RanBP2-type 4 | ||||
Sequence: RDEPWTCKKCTLVNYSTAMACVVCGGSKLK | ||||||
Zinc finger | 744-774 | RanBP2-type 5 | ||||
Sequence: RKGEFWTCSHCTLKNSLHSPVCSACKSHRQP | ||||||
Region | 786-811 | Disordered | ||||
Sequence: RPDGQSYEEQDAAAVGGGGGSAHQSG | ||||||
Compositional bias | 860-876 | Polar residues | ||||
Sequence: SLQQQRNSSSSGAIPKR | ||||||
Region | 860-884 | Disordered | ||||
Sequence: SLQQQRNSSSSGAIPKRHSTGGSIV | ||||||
Zinc finger | 927-956 | RanBP2-type 6 | ||||
Sequence: STKKWQCPACTYDNCAASVVCDICSSPRGL | ||||||
Domain | 1014-1321 | Calpain catalytic | ||||
Sequence: LFVDDSFPPAPKSLYYNPASGAGEGNPVVQWRRPHEINCDGGAYPPWAVFRTPLPSDICQGVLGNCWLLSALAVLAEREDLVKEVLVTKEICGQGAYQVRLCKDGKWTTVLVDDLLPCDKRGHLVYSQAKRKQLWVPLIEKAVAKIHGCYEALVSGRAIEGLATLTGAPCESIPLQASSLPMPSEDELDKDLIWAQLLSSRCVRFLMGASCGGGNMKVDEEEYQQKGLRPRHAYSVLDVKDIQGHRLLKLRNPWGHYSWRGDWSDDSSLWTDDLRDALMPHGASEGVFWISFEDVLNYFDCIDICKVR |
Sequence similarities
Belongs to the peptidase C2 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
P27398-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameB
- Length1,594
- Mass (Da)174,313
- Last updated2004-10-25 v2
- Checksum1CDDE8090B5DA198
P27398-2
- NameA
P27398-5
- NameE
- Differences from canonical
- 701-701: Missing
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
E1JJ98 | E1JJ98_DROME | sol | 1593 |
Sequence caution
Features
Showing features for compositional bias, sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 234-256 | Polar residues | ||||
Sequence: GSGSGPGSGSGLRRTQSLSTAID | ||||||
Sequence conflict | 384 | in Ref. 1; AAB95431 | ||||
Sequence: Q → H | ||||||
Alternative sequence | VSP_011791 | 394-395 | in isoform A | |||
Sequence: VS → YA | ||||||
Alternative sequence | VSP_005246 | 396-1594 | in isoform A | |||
Sequence: Missing | ||||||
Compositional bias | 439-459 | Polar residues | ||||
Sequence: SNSNSSGNSNIINNNSSSSSG | ||||||
Compositional bias | 529-543 | Polar residues | ||||
Sequence: IASESQTNNNTGSGE | ||||||
Compositional bias | 569-594 | Basic and acidic residues | ||||
Sequence: SESQVEEHSIYAKVWKGPRKATESKI | ||||||
Sequence conflict | 623 | in Ref. 1; AAB95431 | ||||
Sequence: A → G | ||||||
Sequence conflict | 679 | in Ref. 1; AAB95431 | ||||
Sequence: Q → H | ||||||
Sequence conflict | 694 | in Ref. 1; AAB95431 and 2; AAC28409 | ||||
Sequence: H → HHHH | ||||||
Alternative sequence | VSP_058143 | 701 | in isoform E | |||
Sequence: Missing | ||||||
Sequence conflict | 836 | in Ref. 5; AAM75061 | ||||
Sequence: Q → R | ||||||
Compositional bias | 860-876 | Polar residues | ||||
Sequence: SLQQQRNSSSSGAIPKR | ||||||
Sequence conflict | 915 | in Ref. 2; AAC28409 | ||||
Sequence: A → G | ||||||
Sequence conflict | 928 | in Ref. 1; AAB95431 and 2; AAC28409 | ||||
Sequence: T → S |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M64084 EMBL· GenBank· DDBJ | AAB95431.1 EMBL· GenBank· DDBJ | mRNA | ||
AF017777 EMBL· GenBank· DDBJ | AAC28409.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014298 EMBL· GenBank· DDBJ | AAF50826.4 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014298 EMBL· GenBank· DDBJ | AAF50827.3 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014298 EMBL· GenBank· DDBJ | AAS65411.2 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
AY128468 EMBL· GenBank· DDBJ | AAM75061.1 EMBL· GenBank· DDBJ | mRNA |