P27136 · TFDE1_CUPPJ
- ProteinCarboxymethylenebutenolidase 1
- GenetfdEI
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids234 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Ring cleavage of cyclic ester dienelactone to produce maleylacetate.
Miscellaneous
Carboxymethylenebutenolidase is specific for dienelactone and has no activity toward enol-lactones.
Catalytic activity
- 2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-2-enedioate + H+
Pathway
Aromatic compound metabolism; 3-chlorocatechol degradation.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 123 | |||||
Sequence: C | ||||||
Active site | 171 | |||||
Sequence: D | ||||||
Active site | 201 | |||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carboxymethylenebutenolidase activity | |
Biological Process | catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCarboxymethylenebutenolidase 1
- EC number
- Alternative names
Gene names
Encoded on
- Plasmid pJP4
- Plasmid pPJ4
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Cupriavidus
Accessions
- Primary accessionP27136
- Secondary accessions
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000161574 | 1-234 | Carboxymethylenebutenolidase 1 | |||
Sequence: MLSDGVEITSRSGGRFGAYLGKPTTDSAPIVVIAQEIFGITPFIRETVEWLVGAGFGCVCPDLYWRQAPNIELDANVPSEREQALALFRDFDMEAGVNDLSCAIEYARALPFSNGRVAVVGYCLGGALAFDVAARSLADCSIGYYGVGLEKKVSLVPAITRPAMFHMGTKDHYVTEEARSILEEHFGRNKNLSLHWYPVGHSFARSSSPNFDQAATTVANARTLELLAMLKDPS |
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Length234
- Mass (Da)25,428
- Last updated1992-08-01 v1
- Checksum1D0658C527CA87BA
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M35097 EMBL· GenBank· DDBJ | AAA98264.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY365053 EMBL· GenBank· DDBJ | AAR31037.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP000093 EMBL· GenBank· DDBJ | AAZ65762.1 EMBL· GenBank· DDBJ | Genomic DNA |