P26309 · CDC20_YEAST
- ProteinAPC/C activator protein CDC20
- GeneCDC20
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids610 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). At the metaphase-to-anaphase transition, recognizes and binds proteins containing a D-box including the B-type cyclins CLB2 and CLB5, HSL1 and securin PDS1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for sister chromatid separation and disassembly of the mitotic spindle. Target of the spindle checkpoint pathway through participation in the mitotic checkpoint complex (MCC) and the MAD2-CDC20 subcomplex. MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20 until proper attachment of all chromosomes to the spindle.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | anaphase-promoting complex | |
Cellular Component | cytoplasm | |
Cellular Component | mitotic checkpoint complex | |
Cellular Component | mitotic checkpoint complex, CDC20-MAD2 subcomplex | |
Cellular Component | nuclear periphery | |
Cellular Component | nucleus | |
Molecular Function | anaphase-promoting complex binding | |
Molecular Function | ubiquitin ligase activator activity | |
Biological Process | anaphase-promoting complex-dependent catabolic process | |
Biological Process | cell division | |
Biological Process | meiotic cell cycle | |
Biological Process | mitotic spindle assembly checkpoint signaling | |
Biological Process | positive regulation of anaphase-promoting complex-dependent catabolic process | |
Biological Process | positive regulation of mitotic metaphase/anaphase transition | |
Biological Process | positive regulation of protein ubiquitination | |
Biological Process | protein ubiquitination | |
Biological Process | regulation of meiotic nuclear division | |
Biological Process | regulation of mitotic cell cycle |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAPC/C activator protein CDC20
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP26309
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 205 | Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. | ||||
Sequence: Y → N | ||||||
Mutagenesis | 209 | Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. | ||||
Sequence: P → Q | ||||||
Mutagenesis | 210 | Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3. | ||||
Sequence: P → L or S |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 8 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000050903 | 1-610 | APC/C activator protein CDC20 | |||
Sequence: MPESSRDKGNAAISGNRSVLSIASPTKLNILSSDWSRNQGKVSKNSLKRSSSLNIRNSKRPSLQASANSIYSRPKITIGAPPLIRRDSSFFKDEFDAKKDKATFSAYSSRSYPTIGSESVVSQTSLSQPTTSREVDEQFTVAADRYIPILQGASQNKVDPETLHEALPPPNASPISHLRAQTKIVFKQNVAEACGLDMNKRILQYMPEPPKCSSLRQKSYIMKKRTHYSYQQEQKIPDLIKLRKINTNPERILDAPGFQDDFYLNLLSWSKKNVLAIALDTALYLWNATTGDVSLLTDFENTTICSVTWSDDDCHISIGKEDGNTEIWDVETMSLIRTMRSGLGVRIGSLSWLDTLIATGSRSGEIQINDVRIKQHIVSTWAEHTGEVCGLSYKSDGLQLASGGNDNTVMIWDTRTSLPQFSKKTHTAAVKALSWCPYSPNILASGGGQTDKHIHFWNSITGARVGSINTGSQVSSLHWGQSHTSTNGGMMNKEIVATGGNPENAISVYNYETKFKVAEVVHAHEARICCSQLSPDGTTLATVGGDENLKFYKIFDPRCTGRSREDGLMDGMLGLIGKEGCRTNDKENRSKNSSEIHTRRPSSTSQYLIR |
Post-translational modification
Ubiquitinated by the APC/C complex.
Phosphorylated by CDC28.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Developmental stage
Expressed in late S and M phase of the cell cycle. Degraded in G1 by the APC/C in its CDH1-bound form.
Interaction
Subunit
Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 subcomplex, both of which appear to be assembled during mitoisis independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 occurs in a larger amount than MCC. MCC associates with the with the APC/C complex. Interacts with MAD2, MAD3, BUB3, HSL1 and PDS1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P26309 | BUB1 P41695 | 2 | EBI-4212, EBI-3816 | |
BINARY | P26309 | BUB3 P26449 | 8 | EBI-4212, EBI-3830 | |
BINARY | P26309 | MAD2 P40958 | 6 | EBI-4212, EBI-10362 | |
BINARY | P26309 | MAD3 P47074 | 9 | EBI-4212, EBI-10369 |
Complex viewer
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for motif, region, repeat, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 17-25 | D-box | ||||
Sequence: RSVLSIASP | ||||||
Region | 38-59 | Disordered | ||||
Sequence: NQGKVSKNSLKRSSSLNIRNSK | ||||||
Motif | 85-101 | Bipartite nuclear localization signal | ||||
Sequence: RRDSSFFKDEFDAKKDK | ||||||
Motif | 144-150 | C-box | ||||
Sequence: DRYIPIL | ||||||
Repeat | 257-296 | WD 1 | ||||
Sequence: GFQDDFYLNLLSWSKKNVLAIALDTALYLWNATTGDVSLL | ||||||
Repeat | 299-338 | WD 2 | ||||
Sequence: FENTTICSVTWSDDDCHISIGKEDGNTEIWDVETMSLIRT | ||||||
Repeat | 342-379 | WD 3 | ||||
Sequence: GLGVRIGSLSWLDTLIATGSRSGEIQINDVRIKQHIVS | ||||||
Repeat | 383-422 | WD 4 | ||||
Sequence: EHTGEVCGLSYKSDGLQLASGGNDNTVMIWDTRTSLPQFS | ||||||
Repeat | 425-467 | WD 5 | ||||
Sequence: THTAAVKALSWCPYSPNILASGGGQTDKHIHFWNSITGARVGS | ||||||
Repeat | 469-519 | WD 6 | ||||
Sequence: NTGSQVSSLHWGQSHTSTNGGMMNKEIVATGGNPENAISVYNYETKFKVAE | ||||||
Repeat | 523-562 | WD 7 | ||||
Sequence: AHEARICCSQLSPDGTTLATVGGDENLKFYKIFDPRCTGR | ||||||
Region | 579-610 | Disordered | ||||
Sequence: EGCRTNDKENRSKNSSEIHTRRPSSTSQYLIR | ||||||
Compositional bias | 580-596 | Basic and acidic residues | ||||
Sequence: GCRTNDKENRSKNSSEI | ||||||
Motif | 586-592 | KEN box | ||||
Sequence: KENRSKN |
Domain
The KEN box is required for the association with the APC/C complex.
The C-box is required for the association with the APC/C complex.
The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
Sequence similarities
Belongs to the WD repeat CDC20/Fizzy family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length610
- Mass (Da)67,360
- Last updated1994-02-01 v2
- ChecksumCCE7CD149C1F5ACF
Sequence caution
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 318-319 | in Ref. 1; CAA42058 | ||||
Sequence: IG → MA | ||||||
Compositional bias | 580-596 | Basic and acidic residues | ||||
Sequence: GCRTNDKENRSKNSSEI |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X59428 EMBL· GenBank· DDBJ | CAA42058.1 EMBL· GenBank· DDBJ | Genomic DNA | Frameshift | |
Z72638 EMBL· GenBank· DDBJ | CAA96824.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
D16506 EMBL· GenBank· DDBJ | BAA03957.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006941 EMBL· GenBank· DDBJ | DAA07992.1 EMBL· GenBank· DDBJ | Genomic DNA |