P26309 · CDC20_YEAST

Function

function

Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). At the metaphase-to-anaphase transition, recognizes and binds proteins containing a D-box including the B-type cyclins CLB2 and CLB5, HSL1 and securin PDS1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for sister chromatid separation and disassembly of the mitotic spindle. Target of the spindle checkpoint pathway through participation in the mitotic checkpoint complex (MCC) and the MAD2-CDC20 subcomplex. MCC and presumably the MAD2-CDC20 subcomplex inhibit the ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) by preventing its activation by CDC20 until proper attachment of all chromosomes to the spindle.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentanaphase-promoting complex
Cellular Componentcytoplasm
Cellular Componentmitotic checkpoint complex
Cellular Componentmitotic checkpoint complex, CDC20-MAD2 subcomplex
Cellular Componentnuclear periphery
Cellular Componentnucleus
Molecular Functionanaphase-promoting complex binding
Molecular Functionubiquitin ligase activator activity
Biological Processanaphase-promoting complex-dependent catabolic process
Biological Processcell division
Biological Processmeiotic cell cycle
Biological Processmitotic spindle assembly checkpoint signaling
Biological Processpositive regulation of anaphase-promoting complex-dependent catabolic process
Biological Processpositive regulation of mitotic metaphase/anaphase transition
Biological Processpositive regulation of protein ubiquitination
Biological Processprotein ubiquitination
Biological Processregulation of meiotic nuclear division
Biological Processregulation of mitotic cell cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    APC/C activator protein CDC20
  • Alternative names
    • Cell division control protein 20

Gene names

    • Name
      CDC20
    • Ordered locus names
      YGL116W

Organism names

Accessions

  • Primary accession
    P26309
  • Secondary accessions
    • D6VU31

Proteomes

Organism-specific databases

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis205Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3.
Mutagenesis209Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3.
Mutagenesis210Confers spindle checkpoint resistance and diminishes binding to MAD2 and MAD3.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 8 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000509031-610APC/C activator protein CDC20

Post-translational modification

Ubiquitinated by the APC/C complex.
Phosphorylated by CDC28.

Keywords

Proteomic databases

PTM databases

Expression

Developmental stage

Expressed in late S and M phase of the cell cycle. Degraded in G1 by the APC/C in its CDH1-bound form.

Interaction

Subunit

Component of the mitotic checkpoint complex (MCC) which consists of MAD2, MAD3, BUB3 and CDC20, and of the MAD2-CDC20 subcomplex, both of which appear to be assembled during mitoisis independently of the kinetochore. In mitose-arrested cells, MAD2-CDC20 occurs in a larger amount than MCC. MCC associates with the with the APC/C complex. Interacts with MAD2, MAD3, BUB3, HSL1 and PDS1.

Binary interactions

Complex viewer

View interactors in UniProtKB
View CPX-760 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for motif, region, repeat, compositional bias.

TypeIDPosition(s)Description
Motif17-25D-box
Region38-59Disordered
Motif85-101Bipartite nuclear localization signal
Motif144-150C-box
Repeat257-296WD 1
Repeat299-338WD 2
Repeat342-379WD 3
Repeat383-422WD 4
Repeat425-467WD 5
Repeat469-519WD 6
Repeat523-562WD 7
Region579-610Disordered
Compositional bias580-596Basic and acidic residues
Motif586-592KEN box

Domain

The KEN box is required for the association with the APC/C complex.
The C-box is required for the association with the APC/C complex.
The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.

Sequence similarities

Belongs to the WD repeat CDC20/Fizzy family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    610
  • Mass (Da)
    67,360
  • Last updated
    1994-02-01 v2
  • Checksum
    CCE7CD149C1F5ACF
MPESSRDKGNAAISGNRSVLSIASPTKLNILSSDWSRNQGKVSKNSLKRSSSLNIRNSKRPSLQASANSIYSRPKITIGAPPLIRRDSSFFKDEFDAKKDKATFSAYSSRSYPTIGSESVVSQTSLSQPTTSREVDEQFTVAADRYIPILQGASQNKVDPETLHEALPPPNASPISHLRAQTKIVFKQNVAEACGLDMNKRILQYMPEPPKCSSLRQKSYIMKKRTHYSYQQEQKIPDLIKLRKINTNPERILDAPGFQDDFYLNLLSWSKKNVLAIALDTALYLWNATTGDVSLLTDFENTTICSVTWSDDDCHISIGKEDGNTEIWDVETMSLIRTMRSGLGVRIGSLSWLDTLIATGSRSGEIQINDVRIKQHIVSTWAEHTGEVCGLSYKSDGLQLASGGNDNTVMIWDTRTSLPQFSKKTHTAAVKALSWCPYSPNILASGGGQTDKHIHFWNSITGARVGSINTGSQVSSLHWGQSHTSTNGGMMNKEIVATGGNPENAISVYNYETKFKVAEVVHAHEARICCSQLSPDGTTLATVGGDENLKFYKIFDPRCTGRSREDGLMDGMLGLIGKEGCRTNDKENRSKNSSEIHTRRPSSTSQYLIR

Sequence caution

The sequence CAA42058.1 differs from that shown. Reason: Frameshift

Features

Showing features for sequence conflict, compositional bias.

TypeIDPosition(s)Description
Sequence conflict318-319in Ref. 1; CAA42058
Compositional bias580-596Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X59428
EMBL· GenBank· DDBJ
CAA42058.1
EMBL· GenBank· DDBJ
Genomic DNA Frameshift
Z72638
EMBL· GenBank· DDBJ
CAA96824.1
EMBL· GenBank· DDBJ
Genomic DNA
D16506
EMBL· GenBank· DDBJ
BAA03957.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006941
EMBL· GenBank· DDBJ
DAA07992.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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