P26149 · 3BHS2_MOUSE
- Protein3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
- GeneHsd3b2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids373 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta5-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Catalytic activity
- a 3beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + H+ + NADH
- NAD+ + pregnenolone = H+ + NADH + pregn-5-ene-3,20-dioneThis reaction proceeds in the forward direction.
- pregn-5-ene-3,20-dione = progesteroneThis reaction proceeds in the forward direction.
- androst-5-ene-3,17-dione = androst-4-ene-3,17-dioneThis reaction proceeds in the forward direction.
Pathway
Lipid metabolism; steroid biosynthesis.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | intracellular membrane-bounded organelle | |
Cellular Component | mitochondrial membrane | |
Molecular Function | 3-beta-hydroxy-delta5-steroid dehydrogenase (NAD+) activity | |
Molecular Function | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | |
Molecular Function | steroid delta-isomerase activity | |
Biological Process | androgen biosynthetic process | |
Biological Process | C21-steroid hormone biosynthetic process | |
Biological Process | C21-steroid hormone metabolic process | |
Biological Process | hippocampus development | |
Biological Process | negative regulation of iron ion transport | |
Biological Process | response to corticosterone | |
Biological Process | steroid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
- Alternative names
Including 2 domains:
- Recommended name3-beta-hydroxy-Delta(5)-steroid dehydrogenase
- EC number
- Alternative names
- Recommended nameSteroid Delta-isomerase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP26149
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Mitochondrion membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 288-308 | Helical | ||||
Sequence: VPLLYWLAFLLETVSFLLSPI |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000087781 | 1-373 | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2 | |||
Sequence: MPGWSCLVTGAGGFLGQRIIQLLVQEEDLEEIRVLDKVFRPETRKEFFNLETSIKVTVLEGDILDTQYLRRACQGISVVIHTAAIIDVTGVIPRQTILDVNLKGTQNLLEACIQASVPAFIFSSSVDVAGPNSYKEIVLNGHEEECHESTWSDPYPYSKKMAEKAVLAANGSMLKNGGTLQTCALRPMCIYGERSPLISNIIIMALKHKGILRSFGKFNTANPVYVGNVAWAHILAARGLRDPKKSPNIQGEFYYISDDTPHQSFDDISYTLSKEWGFCLDSSWSLPVPLLYWLAFLLETVSFLLSPIYRYIPPFNRHLVTLSGSTFTFSYKKAQRDLGYEPLVSWEEAKQKTSEWIGTLVEQHRETLDTKSQ |
Proteomic databases
PTM databases
Expression
Structure
Sequence
- Sequence statusComplete
- Length373
- Mass (Da)41,923
- Last updated2011-06-28 v4
- Checksum2500AF16F38C081B
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 12 | in Ref. 2; AAH26757/AAH40397 | ||||
Sequence: G → K | ||||||
Sequence conflict | 54 | in Ref. 2; AAH26757/AAH40397 | ||||
Sequence: I → N |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL606755 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC026757 EMBL· GenBank· DDBJ | AAH26757.1 EMBL· GenBank· DDBJ | mRNA | ||
BC040397 EMBL· GenBank· DDBJ | AAH40397.1 EMBL· GenBank· DDBJ | mRNA | ||
M75886 EMBL· GenBank· DDBJ | - | mRNA | No translation available. |