P25779 · CYSP_TRYCR
- ProteinCruzipain
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids467 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Hydrolyzes chromogenic peptides at the carboxyl Arg or Lys; requires at least one more amino acid, preferably Arg, Phe, Val or Leu, between the terminal Arg or Lys and the amino-blocking group.
The cysteine protease may play an important role in the development and differentiation of the parasites at several stages of their life cycle.
Miscellaneous
Purified cruzipain is able to degrade itself, yielding a complex mixture of small peptides, and a major 25 kDa fragment.
Catalytic activity
Activity regulation
Strongly inhibited by E-64 (L-trans-epoxysuccinylleucylamido(4-guanidino)butane), Leupeptin, and N-alpha-p-tosyl-L-lysine chloromethyl ketone.
Features
Showing features for active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 147 | |||||
Sequence: C | ||||||
Active site | 284 | |||||
Sequence: H | ||||||
Active site | 304 | |||||
Sequence: N | ||||||
Site | 337-338 | Cleavage; by autolysis | ||||
Sequence: GG |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular space | |
Cellular Component | lysosome | |
Molecular Function | cysteine-type endopeptidase activity | |
Biological Process | proteolysis involved in protein catabolic process |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCruzipain
- EC number
- Alternative names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Trypanosoma > Schizotrypanum
Accessions
- Primary accessionP25779
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Features
Showing features for natural variant.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Natural variant | 9 | in clone 1800-2 | ||||
Sequence: L → S | ||||||
Natural variant | 35 | in clone 1800-2 | ||||
Sequence: T → A | ||||||
Natural variant | 39 | in clone 1800-2 | ||||
Sequence: A → V | ||||||
Natural variant | 51 | in clone 1800-4 | ||||
Sequence: S → N | ||||||
Natural variant | 56 | in clone 1800-2 | ||||
Sequence: A → R | ||||||
Natural variant | 76 | in clone 1800-4 | ||||
Sequence: N → G | ||||||
Natural variant | 109 | in clone 1800-4 | ||||
Sequence: Q → G | ||||||
Natural variant | 117 | in clone 1800-2 | ||||
Sequence: K → N | ||||||
Natural variant | 146 | |||||
Sequence: S → G | ||||||
Natural variant | 157-158 | in strain: RA | ||||
Sequence: EC → SG | ||||||
Natural variant | 186 | in clones 1800-2 and 1800-4 | ||||
Sequence: S → G | ||||||
Natural variant | 204 | in strain: RA | ||||
Sequence: A → G | ||||||
Natural variant | 250-251 | in clones 1800-2 and 1800-4 | ||||
Sequence: WL → CV | ||||||
Natural variant | 261-262 | in strain: RA | ||||
Sequence: VD → H | ||||||
Natural variant | 286 | in clone 1800-4 | ||||
Sequence: V → F | ||||||
Natural variant | 286 | in strain: RA | ||||
Sequence: V → L | ||||||
Natural variant | 308 | in clone 1800-2 | ||||
Sequence: T → A | ||||||
Natural variant | 313 | |||||
Sequence: E → D | ||||||
Natural variant | 409 | in clone 1800-2 | ||||
Sequence: L → F | ||||||
Natural variant | 427 | in clone 1800-2 | ||||
Sequence: K → Q | ||||||
Natural variant | 430 | in clone 1800-2 | ||||
Sequence: R → W | ||||||
Natural variant | 457 | in clone 1800-2 | ||||
Sequence: H → Y | ||||||
Natural variant | 461 | in clone 1800-2 | ||||
Sequence: H → Q |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 23 variants from UniProt as well as other sources including ClinVar and dbSNP.
Chemistry
PTM/Processing
Features
Showing features for signal, propeptide, chain, disulfide bond, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-18 | |||||
Sequence: MSGWARALLLAAVLVVMA | ||||||
Propeptide | PRO_0000026372 | 19-122 | Activation peptide | |||
Sequence: CLVPAATASLHAEETLTSQFAEFKQKHGRVYESAAEEAFRLSVFRENLFLARLHAAANPHATFGVTPFSDLTREEFRSRYHNGAAHFAAAQERARVPVKVEVVG | ||||||
Chain | PRO_0000026373 | 123-467 | Cruzipain | |||
Sequence: APAAVDWRARGAVTAVKDQGQCGSCWAFSAIGNVECQWFLAGHPLTNLSEQMLVSCDKTDSGCSGGLMNNAFEWIVQENNGAVYTEDSYPYASGEGISPPCTTSGHTVGATITGHVELPQDEAQIAAWLAVNGPVAVAVDASSWMTYTGGVMTSCVSEQLDHGVLLVGYNDSAAVPYWIIKNSWTTQWGEEGYIRIAKGSNQCLVKEEASSAVVGGPGPTPEPTTTTTTSAPGPSPSYFVQMSCTDAACIVGCENVTLPTGQCLLTTSGVSAIVTCGAETLTEEVFLTSTHCSGPSVRSSVPLNKCNRLLRGSVEFFCGSSSSGRLADVDRQRRHQPYHSRHRRL | ||||||
Disulfide bond | 144↔185 | |||||
Sequence: CGSCWAFSAIGNVECQWFLAGHPLTNLSEQMLVSCDKTDSGC | ||||||
Glycosylation | 169 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 178↔223 | |||||
Sequence: CDKTDSGCSGGLMNNAFEWIVQENNGAVYTEDSYPYASGEGISPPC | ||||||
Disulfide bond | 277↔325 | |||||
Sequence: CVSEQLDHGVLLVGYNDSAAVPYWIIKNSWTTQWGEEGYIRIAKGSNQC | ||||||
Glycosylation | 292 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 377 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Expression
Developmental stage
Present in all developmental stages.
Interaction
Chemistry
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length467
- Mass (Da)49,836
- Last updated1992-05-01 v1
- ChecksumB93EBA49B511363D
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 357 | in Ref. 4 | ||||
Sequence: S → C |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M84342 EMBL· GenBank· DDBJ | AAA30181.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M69121 EMBL· GenBank· DDBJ | AAA30269.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M69121 EMBL· GenBank· DDBJ | AAA30270.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X54414 EMBL· GenBank· DDBJ | CAA38278.1 EMBL· GenBank· DDBJ | mRNA | ||
M27305 EMBL· GenBank· DDBJ | AAA30180.1 EMBL· GenBank· DDBJ | Genomic DNA |