P25604 · STP22_YEAST
- ProteinSuppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease
- GeneSTP22
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids385 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association to the ESCRT-0 complex. Required for vacuolar targeting of temperature-sensitive plasma membrane proteins STE2 and CAN1.
Miscellaneous
Present with 1360 molecules/cell in log phase SD medium.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasmic side of plasma membrane | |
Cellular Component | cytosol | |
Cellular Component | endosome | |
Cellular Component | ESCRT I complex | |
Cellular Component | late endosome membrane | |
Molecular Function | ubiquitin binding | |
Biological Process | ATP export | |
Biological Process | late endosome to vacuole transport | |
Biological Process | negative regulation of protein polyubiquitination | |
Biological Process | protein modification process | |
Biological Process | protein targeting to membrane | |
Biological Process | protein targeting to vacuole | |
Biological Process | reticulophagy | |
Biological Process | ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameSuppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP25604
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Late endosome membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 85 | No interaction of the ESCRT-I complex with ubiquitin. | |||
Mutagenesis | 254 | Defective in ESCRT-I cargo sorting; reduces MVB12 localization to MVBs; abolishes interaction with MVB12; reduces interaction with SRN2. | |||
Mutagenesis | 286 | Defective in ESCRT-I cargo sorting. | |||
Mutagenesis | 345 | Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVBs). | |||
Mutagenesis | 371 | Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVBs). | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 7 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000082605 | 1-385 | Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease | ||
Proteomic databases
PTM databases
Interaction
Subunit
Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of STP22, VPS28, SRN2 and MVB12 in a 1:1:1:1 stoichiometry. Interacts with HSE1 and VPS27. Interacts with MVB12 and SRN2.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | P25604 | HOF1 Q05080 | 2 | EBI-411625, EBI-5412 | |
BINARY | P25604 | HSE1 P38753 | 3 | EBI-411625, EBI-1382 | |
BINARY | P25604 | LSB3 P43603 | 3 | EBI-411625, EBI-22980 | |
BINARY | P25604 | MVB12 P42939 | 13 | EBI-411625, EBI-23478 | |
BINARY | P25604 | PEX13 P80667 | 2 | EBI-411625, EBI-13206 | |
BINARY | P25604 | SRN2 Q99176 | 9 | EBI-411625, EBI-18076 | |
BINARY | P25604 | VPS27 P40343 | 8 | EBI-411625, EBI-20380 | |
BINARY | P25604 | VPS28 Q02767 | 4 | EBI-411625, EBI-20387 | |
BINARY | P25604 | YSC84 P32793 | 2 | EBI-411625, EBI-24460 |
Complex viewer
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias, coiled coil.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 12-161 | UEV | |||
Region | 155-219 | Disordered | |||
Compositional bias | 177-203 | Pro residues | |||
Coiled coil | 272-300 | ||||
Domain | 322-385 | SB | |||
Domain
The UEV domain is required for the interaction of the complex with ubiquitin.
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length385
- Mass (Da)43,330
- Last updated2003-10-24 v3
- ChecksumFEDE3BE79F063BCE
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 177-203 | Pro residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF004731 EMBL· GenBank· DDBJ | AAB62820.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X59720 EMBL· GenBank· DDBJ | CAC42964.1 EMBL· GenBank· DDBJ | Genomic DNA | Frameshift | |
AY260880 EMBL· GenBank· DDBJ | AAP21748.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006937 EMBL· GenBank· DDBJ | DAA07473.1 EMBL· GenBank· DDBJ | Genomic DNA |