P25604 · STP22_YEAST

Function

function

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association to the ESCRT-0 complex. Required for vacuolar targeting of temperature-sensitive plasma membrane proteins STE2 and CAN1.

Miscellaneous

Present with 1360 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasmic side of plasma membrane
Cellular Componentcytosol
Cellular Componentendosome
Cellular ComponentESCRT I complex
Cellular Componentlate endosome membrane
Molecular Functionubiquitin binding
Biological ProcessATP export
Biological Processlate endosome to vacuole transport
Biological Processnegative regulation of protein polyubiquitination
Biological Processprotein modification process
Biological Processprotein targeting to membrane
Biological Processprotein targeting to vacuole
Biological Processreticulophagy
Biological Processubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

Keywords

Enzyme and pathway databases

Protein family/group databases

    • 3.A.31.1.1the endosomal sorting complexes required for transport iii (escrt-iii) family

Names & Taxonomy

Protein names

  • Recommended name
    Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease
  • Alternative names
    • ESCRT-I complex subunit VPS23
    • Vacuolar protein sorting-associated protein 23

Gene names

    • Name
      STP22
    • Synonyms
      VPS23
    • ORF names
      YCL8C
    • Ordered locus names
      YCL008C

Organism names

Accessions

  • Primary accession
    P25604
  • Secondary accessions
    • D6VR04
    • P87010
    • P87279
    • Q86ZT3
    • Q8NIM6

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis85No interaction of the ESCRT-I complex with ubiquitin.
Mutagenesis254Defective in ESCRT-I cargo sorting; reduces MVB12 localization to MVBs; abolishes interaction with MVB12; reduces interaction with SRN2.
Mutagenesis286Defective in ESCRT-I cargo sorting.
Mutagenesis345Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVBs).
Mutagenesis371Abolishes ESCRT-I complex assembly; class E phenotype (malformed late MVBs).

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 7 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000826051-385Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease

Proteomic databases

PTM databases

Interaction

Subunit

Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of STP22, VPS28, SRN2 and MVB12 in a 1:1:1:1 stoichiometry. Interacts with HSE1 and VPS27. Interacts with MVB12 and SRN2.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY P25604HOF1 Q050802EBI-411625, EBI-5412
BINARY P25604HSE1 P387533EBI-411625, EBI-1382
BINARY P25604LSB3 P436033EBI-411625, EBI-22980
BINARY P25604MVB12 P4293913EBI-411625, EBI-23478
BINARY P25604PEX13 P806672EBI-411625, EBI-13206
BINARY P25604SRN2 Q991769EBI-411625, EBI-18076
BINARY P25604VPS27 P403438EBI-411625, EBI-20380
BINARY P25604VPS28 Q027674EBI-411625, EBI-20387
BINARY P25604YSC84 P327932EBI-411625, EBI-24460

Complex viewer

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain, region, compositional bias, coiled coil.

Type
IDPosition(s)Description
Domain12-161UEV
Region155-219Disordered
Compositional bias177-203Pro residues
Coiled coil272-300
Domain322-385SB

Domain

The UEV domain is required for the interaction of the complex with ubiquitin.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    385
  • Mass (Da)
    43,330
  • Last updated
    2003-10-24 v3
  • Checksum
    FEDE3BE79F063BCE
MSANGKISVPEAVVNWLFKVIQPIYNDGRTTFHDSLALLDNFHSLRPRTRVFTHSDGTPQLLLSIYGTISTGEDGSSPHSIPVIMWVPSMYPVKPPFISINLENFDMNTISSSLPIQEYIDSNGWIALPILHCWDPAAMNLIMVVQELMSLLHEPPQDQAPSLPPKPNTQLQQEQNTPPLPPKPKSPHLKPPLPPPPPPQPASNALDLMDMDNTDISPTNHHEMLQNLQTVVNELYREDVDYVADKILTRQTVMQESIARFHEIIAIDKNHLRAVEQAIEQTMHSLNAQIDVLTANRAKVQQFSSTSHVDDEDVNSIAVAKTDGLNQLYNLVAQDYALTDTIECLSRMLHRGTIPLDTFVKQGRELARQQFLVRWHIQRITSPLS

Sequence caution

The sequence CAC42964.1 differs from that shown. Reason: Frameshift

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias177-203Pro residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF004731
EMBL· GenBank· DDBJ
AAB62820.1
EMBL· GenBank· DDBJ
Genomic DNA
X59720
EMBL· GenBank· DDBJ
CAC42964.1
EMBL· GenBank· DDBJ
Genomic DNA Frameshift
AY260880
EMBL· GenBank· DDBJ
AAP21748.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006937
EMBL· GenBank· DDBJ
DAA07473.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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