P25376 · AGP1_YEAST
- ProteinGeneral amino acid permease AGP1
- GeneAGP1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids633 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Broad substrate range permease which transports asparagine and glutamine with intermediate specificity. Also transports Ala, Cys, Gly, Ile, Leu, Met, Phe, Ser, Thr, Tyr and Val. Important for the utilization of amino acids as a nitrogen source.
Miscellaneous
Present with 21100 molecules/cell in log phase SD medium.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.23 mM | leucine |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
2.6 nmol/min/mg | for leucine transport |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | membrane | |
Cellular Component | plasma membrane | |
Molecular Function | amino acid transmembrane transporter activity | |
Molecular Function | L-phenylalanine transmembrane transporter activity | |
Molecular Function | L-proline transmembrane transporter activity | |
Biological Process | amino acid transmembrane transport | |
Biological Process | amino acid transport | |
Biological Process | serine import across plasma membrane | |
Biological Process | transmembrane transport |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameGeneral amino acid permease AGP1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP25376
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-124 | Cytoplasmic | |||
Transmembrane | 125-145 | Helical | |||
Topological domain | 146-148 | Extracellular | |||
Transmembrane | 149-169 | Helical | |||
Topological domain | 170-197 | Cytoplasmic | |||
Transmembrane | 198-218 | Helical | |||
Topological domain | 219-231 | Extracellular | |||
Transmembrane | 232-252 | Helical | |||
Topological domain | 253-260 | Cytoplasmic | |||
Transmembrane | 261-281 | Helical | |||
Topological domain | 282-313 | Extracellular | |||
Transmembrane | 314-334 | Helical | |||
Topological domain | 335-352 | Cytoplasmic | |||
Transmembrane | 353-373 | Helical | |||
Topological domain | 374-402 | Extracellular | |||
Transmembrane | 403-425 | Helical | |||
Topological domain | 426-452 | Cytoplasmic | |||
Transmembrane | 453-473 | Helical | |||
Topological domain | 474-477 | Extracellular | |||
Transmembrane | 478-498 | Helical | |||
Topological domain | 499-531 | Cytoplasmic | |||
Transmembrane | 532-552 | Helical | |||
Topological domain | 553-560 | Extracellular | |||
Transmembrane | 561-581 | Helical | |||
Topological domain | 582-633 | Cytoplasmic | |||
Keywords
- Cellular component
Phenotypes & Variants
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 10 variants from UniProt as well as other sources including ClinVar and dbSNP.
Chemistry
PTM/Processing
Features
Showing features for chain, modified residue, cross-link, lipidation.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000054142 | 1-633 | General amino acid permease AGP1 | ||
Modified residue | 6 | Phosphoserine | |||
Cross-link | 11 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | |||
Lipidation | 633 | S-palmitoyl cysteine | |||
Post-translational modification
Palmitoylated by PFA4.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
Induced by transcription factors DAL81 and STP1, which are activated by a signal initiated by the plasma membrane SPS (SSY1-PTR3-SSY5) amino acid sensor system in response to external amino acid levels.
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 32-50 | Polar residues | |||
Region | 32-52 | Disordered | |||
Compositional bias | 86-115 | Polar residues | |||
Region | 86-116 | Disordered | |||
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length633
- Mass (Da)69,671
- Last updated2008-04-29 v3
- Checksum8E19A836C2F6C50F
Sequence caution
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 32-50 | Polar residues | |||
Compositional bias | 86-115 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X59720 EMBL· GenBank· DDBJ | CAA42360.2 EMBL· GenBank· DDBJ | Genomic DNA | Frameshift | |
BK006937 EMBL· GenBank· DDBJ | DAA07460.1 EMBL· GenBank· DDBJ | Genomic DNA |