P25348 · RM32_YEAST

Function

function

Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.

Miscellaneous

Present with 1700 molecules/cell in log phase SD medium.

Features

Showing features for binding site.

118320406080100120140160180
TypeIDPosition(s)Description
Binding site104Zn2+ (UniProtKB | ChEBI)
Binding site107Zn2+ (UniProtKB | ChEBI)
Binding site117Zn2+ (UniProtKB | ChEBI)
Binding site120Zn2+ (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentmitochondrial inner membrane
Cellular Componentmitochondrial large ribosomal subunit
Cellular Componentmitochondrion
Molecular Functionstructural constituent of ribosome
Molecular Functionzinc ion binding
Biological Processmitochondrial translation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Large ribosomal subunit protein bL32m
  • Alternative names
    • 54S ribosomal protein L32, mitochondrial
    • YmL32

Gene names

    • Name
      MRPL32
    • ORF names
      YCR041, YCR3W
    • Ordered locus names
      YCR003W

Organism names

Accessions

  • Primary accession
    P25348
  • Secondary accessions
    • D6VR13

Proteomes

Organism-specific databases

Subcellular Location

Mitochondrion
Note: Mitoribosomes are tethered to the mitochondrial inner membrane and spatially aligned with the membrane insertion machinery through two distinct membrane contact sites, formed by the 21S rRNA expansion segment 96-ES1 and the inner membrane protein MBA1.

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis104-107Impaired processing by the m-AAA protease.
Mutagenesis117Impaired processing by the m-AAA protease.
Mutagenesis120Impaired processing by the m-AAA protease.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 6 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-71Mitochondrion
ChainPRO_000003051872-183Large ribosomal subunit protein bL32m

Post-translational modification

MRPL32 precursor is processed by the m-AAA protease (composed of YTA12/RCA1 and YTA10/AFG3), which cleaves the N-terminal transit peptide (PubMed:16239145, PubMed:21610694).
Cleavage by the m-AAA protease takes place prior to assembly into the large subunit, an essential step for mitochondrial ribosome (mitoribosome) assembly (PubMed:16239145).
Proper processing by the m-AAA protease is dependent on the zinc-binding region within the tightly folded C-terminal domain of MRPL32: zinc-dependent folding halts degradation initiated from the N-terminus and triggers the release of mature MRPL32 (PubMed:21610694).

Proteomic databases

Interaction

Subunit

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S small subunit contains a 15S ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins. bL32m has a zinc binding site.

Complex viewer

View interactors in UniProtKB
View CPX-1602 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    183
  • Mass (Da)
    21,437
  • Last updated
    1992-05-01 v1
  • Checksum
    C0F0D87C23AA2DEA
MNSLIFGKQLAFHKIVPTTAIGWLVPLGNPSLQIPGQKQLGSIHRWLREKLQQDHKDTEDKDFFSNNGILLAVPKKKVSHQKKRQKLYGPGKKQLKMIHHLNKCPSCGHYKRANTLCMYCVGQISHIWKTHTAKEEIKPRQEEELSELDQRVLYPGRRDTKYTKDLKDKDNYLERRVRTLKKD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
S48552
EMBL· GenBank· DDBJ
AAD13857.1
EMBL· GenBank· DDBJ
Genomic DNA
Z11113
EMBL· GenBank· DDBJ
CAA77444.1
EMBL· GenBank· DDBJ
Genomic DNA
X59720
EMBL· GenBank· DDBJ
CAA42340.1
EMBL· GenBank· DDBJ
Genomic DNA
AY693214
EMBL· GenBank· DDBJ
AAT93233.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006937
EMBL· GenBank· DDBJ
DAA07482.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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