P25314 · NIFK_AZOBR
- ProteinNitrogenase molybdenum-iron protein beta chain
- GenenifK
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids521 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.
Miscellaneous
Ala-194 is present instead of the usual Ser that would serve as a ligand for the 8Fe-7S cluster in the oxidized state.
Catalytic activity
- N2 + 8 reduced [2Fe-2S]-[ferredoxin] + 16 ATP + 16 H2O = H2 + 8 oxidized [2Fe-2S]-[ferredoxin] + 2 NH4+ + 16 ADP + 16 phosphate + 6 H+
CHEBI:17997 + 8 RHEA-COMP:10001 + 16 CHEBI:30616 + 16 CHEBI:15377 = CHEBI:18276 + 8 RHEA-COMP:10000 + 2 CHEBI:28938 + 16 CHEBI:456216 + 16 CHEBI:43474 + 6 CHEBI:15378
Cofactor
Note: Binds 1 [8Fe-7S] cluster per heterodimer.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 77 | [8Fe-7S] cluster (UniProtKB | ChEBI); ligand shared with alpha chain | |||
Binding site | 102 | [8Fe-7S] cluster (UniProtKB | ChEBI); ligand shared with alpha chain | |||
Binding site | 159 | [8Fe-7S] cluster (UniProtKB | ChEBI); ligand shared with alpha chain | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | molybdenum-iron nitrogenase complex | |
Molecular Function | ATP binding | |
Molecular Function | iron-sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | nitrogenase activity | |
Biological Process | nitrogen fixation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNitrogenase molybdenum-iron protein beta chain
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rhodospirillales > Azospirillaceae > Azospirillum
Accessions
- Primary accessionP25314
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000153087 | 1-521 | Nitrogenase molybdenum-iron protein beta chain | ||
Interaction
Subunit
Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).
Structure
Sequence
- Sequence statusComplete
- Length521
- Mass (Da)58,216
- Last updated1992-05-01 v1
- MD5 Checksum45EC0ECA4BA8B4FB6DD8643EC123219E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M64344 EMBL· GenBank· DDBJ | AAB02344.1 EMBL· GenBank· DDBJ | Genomic DNA |