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P25314 · NIFK_AZOBR

  • Protein
    Nitrogenase molybdenum-iron protein beta chain
  • Gene
    nifK
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.

Miscellaneous

Ala-194 is present instead of the usual Ser that would serve as a ligand for the 8Fe-7S cluster in the oxidized state.

Catalytic activity

Cofactor

[8Fe-7S] cluster (UniProtKB | Rhea| CHEBI:21143 )

Note: Binds 1 [8Fe-7S] cluster per heterodimer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site77[8Fe-7S] cluster (UniProtKB | ChEBI); ligand shared with alpha chain
Binding site102[8Fe-7S] cluster (UniProtKB | ChEBI); ligand shared with alpha chain
Binding site159[8Fe-7S] cluster (UniProtKB | ChEBI); ligand shared with alpha chain

GO annotations

AspectTerm
Cellular Componentmolybdenum-iron nitrogenase complex
Molecular FunctionATP binding
Molecular Functioniron-sulfur cluster binding
Molecular Functionmetal ion binding
Molecular Functionnitrogenase activity
Biological Processnitrogen fixation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Nitrogenase molybdenum-iron protein beta chain
  • EC number
  • Alternative names
    • Dinitrogenase
    • Nitrogenase component I

Gene names

    • Name
      nifK

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Rhodospirillales > Azospirillaceae > Azospirillum

Accessions

  • Primary accession
    P25314

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001530871-521Nitrogenase molybdenum-iron protein beta chain

Interaction

Subunit

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).

Structure

Family & Domains

Sequence similarities

Belongs to the NifD/NifK/NifE/NifN family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    521
  • Mass (Da)
    58,216
  • Last updated
    1992-05-01 v1
  • MD5 Checksum
    45EC0ECA4BA8B4FB6DD8643EC123219E
MSMSHPVSQSADKVIDHFTLFRQPEYKELFERKKTEFEYGPTPTKEVARVSAWTKTEEYKEKNLPVEAVVINPTKACQPIGAMLAAQGFEGTLPFVHGSQGCVSYYRTHLTRHFKEPNSAVSSSMTEDAAVFGGLNNMIDGLQRYALYKPKMIAVLTTCMAEVIGDDLSGFINNAKNKESVPADFPVPFAHTPAFVGSHIVGYDNMIKGVLTHFWGTSENFDTPKNETINLIPGFDGFAVGNNRELKRIAGLFGIQMTILSDVSDNFDTPADGEYRMYDGGTPLEATKEAVHAKATISMQEYCTPQSLQFIKREGPAGRQAYNYPMGVTGTDELLMKLAELSGKPSRGVKLERGRLVDAIADSHTHLHGKRFAVYGDPDFCLGMSKFLMELGAEPVHILSTSGSKKWEKQVQKVLDACRSASSGKAYGAKDLWHLRSLVFTDKVDYIIGNSYGKYLERDTKIPLIRLTYPIFDRHHHHRYPTWGYQGALNVLVRILDRIFEDMDANTNIVGETDYSFDLVR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M64344
EMBL· GenBank· DDBJ
AAB02344.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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