P25239 · T257_ECOLX
- ProteinType II restriction enzyme and methyltransferase RM.Eco57I
- Geneeco57IR
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids998 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
An E, G and S subtype restriction enzyme that recognizes the (non-palindromic) double-stranded sequence 5'-CTGAAG-3' and cleaves respectively 22 bases after C-1 and 14 bases before C'-1; cleavage of lambda DNA is never complete. Also acts as a methylase that causes specific methylation on A-5 in 5'-CTGAAG-3', the other strand is methylated by the M.Eco57I methylase.
Catalytic activity
- a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H+
Activity regulation
Mg2+ is absolutely required for DNA restriction.
pH Dependence
Optimum pH is 9.0 for DNA restriction and 7.5 for methylation.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA binding | |
Molecular Function | site-specific DNA-methyltransferase (adenine-specific) activity | |
Molecular Function | type II site-specific deoxyribonuclease activity | |
Biological Process | DNA restriction-modification system | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameType II restriction enzyme and methyltransferase RM.Eco57I
- EC number
- Alternative names
Including 1 domain:
- Recommended nameAdenine-specific methyltransferase activity Eco57IA
- EC number
- Short namesM.Eco57IA
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP25239
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000077274 | 1-998 | Type II restriction enzyme and methyltransferase RM.Eco57I | ||
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Length998
- Mass (Da)117,087
- Last updated2004-09-13 v2
- ChecksumEDB090A7FBFFCC93
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 42 | in Ref. 1; AAA23389 | |||
Sequence conflict | 207 | in Ref. 1; AAA23389 | |||
Sequence conflict | 299-321 | in Ref. 1; AAA23389 | |||
Sequence conflict | 401 | in Ref. 1; AAA23389 | |||
Sequence conflict | 644 | in Ref. 1; AAA23389 | |||
Sequence conflict | 816-823 | in Ref. 1; AAA23389 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M74821 EMBL· GenBank· DDBJ | AAA23389.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X61122 EMBL· GenBank· DDBJ | CAA43434.3 EMBL· GenBank· DDBJ | Genomic DNA |