P25239 · T257_ECOLX

  • Protein
    Type II restriction enzyme and methyltransferase RM.Eco57I
  • Gene
    eco57IR
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

An E, G and S subtype restriction enzyme that recognizes the (non-palindromic) double-stranded sequence 5'-CTGAAG-3' and cleaves respectively 22 bases after C-1 and 14 bases before C'-1; cleavage of lambda DNA is never complete. Also acts as a methylase that causes specific methylation on A-5 in 5'-CTGAAG-3', the other strand is methylated by the M.Eco57I methylase.

Catalytic activity

Activity regulation

Mg2+ is absolutely required for DNA restriction.

pH Dependence

Optimum pH is 9.0 for DNA restriction and 7.5 for methylation.

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular Functionsite-specific DNA-methyltransferase (adenine-specific) activity
Molecular Functiontype II site-specific deoxyribonuclease activity
Biological ProcessDNA restriction-modification system
Biological Processmethylation

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Type II restriction enzyme and methyltransferase RM.Eco57I
  • EC number
  • Alternative names
    • Endonuclease Eco57I
    • Type IIS restriction enzyme Eco57I

Including 1 domain:

  • Recommended name
    Adenine-specific methyltransferase activity Eco57IA
  • EC number
  • Short names
    M.Eco57IA

Gene names

    • Name
      eco57IR

Organism names

  • Taxonomic identifier
  • Strain
    • RFL57
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P25239

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000772741-998Type II restriction enzyme and methyltransferase RM.Eco57I

Interaction

Subunit

Monomer.

Structure

Family & Domains

Sequence similarities

In the C-terminal section; belongs to the N(4)/N(6)-methyltransferase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    998
  • Mass (Da)
    117,087
  • Last updated
    2004-09-13 v2
  • Checksum
    EDB090A7FBFFCC93
MNKKDQLQRLIDKYKSDIDYYRSARYNETQLRTDFLDQLFLILGWDITNAAGKPTNEREVLVEEGLKARAGENTKKPDYTFRLFSERKFFLEAKKPSVDISTTIEPALQVRRYGFTAKLKISVLSNFEYTAIYDCSNQVKETDSVANSRIKLYHFTELVDKFDEINNLIGRESVYTGHFDNEWSEIENKILRFSVDDLFLKQINDWRLLLANEFLQIKNELPEEKLNDLVQNYINSIVFLRVCEDRDLEEYETLYHFAQDKDFQSLVKKLKSSDKKYNSGLFSLEYIDELLSNANSCIWSIIEQLYFPQSTYSFSVFSSDILGNIYEIFLSEKVRIDELGNVKIQPKEEHIDRDVVTTPTHIVKEIIRNTVVEYCKGKSDIEILNSKFADIACGSGAFIIEAFQFIQDILIDYYIQNDKSKLQQISEHTYKLKFEVKREILCKCIYGIDKDYNATKACTFGLLLKLLEGETTETIGKDTPILPALDTNILFGNSLIDSGDKVKQEDIFSINPFDLTNYQFDVIVGNPPYMATEHMNQLTPKELDIYKRKYKSAYKQFDKYFLFIERSIQILKEYGYLGYILPSRFIKVDAGKKLRKFLSENKYLSKLISFGSHQVFKNKTTYTCLLFLNKENHDNFSFYEVKDFKKWLTREDKYLLSSTYQTSSLDSDTWVLEKKINDILKLMFSKSEQLGNIVGKSNVANGIQTSANKYYIHKEIKSENGFIYFEYDGIEYHIEKELTRPYFETNRSGDDSFYTYKDVEPNSFVVYPYKKVGERIQFIEYDELKRQYPKLFEFLQVVKVHLNDKKRSIKPDPTGPNEWYRYGRSQALENCDVDQKLIVGILSNGYKYSIDNHRTFVSSGGTAGYSIINIPNNVRYSIYYIQAILTSKYLEWFASIYGDIFRGRFVARGTKVQTRMPIPTIDFDDPKQKEIHDTISSKQQYLNKLYSQTQKSADRDKIIFERQFEQEKIQMDYLIKNLFDLGDLDSEIPTVEDLYKNL

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict42in Ref. 1; AAA23389
Sequence conflict207in Ref. 1; AAA23389
Sequence conflict299-321in Ref. 1; AAA23389
Sequence conflict401in Ref. 1; AAA23389
Sequence conflict644in Ref. 1; AAA23389
Sequence conflict816-823in Ref. 1; AAA23389

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M74821
EMBL· GenBank· DDBJ
AAA23389.1
EMBL· GenBank· DDBJ
Genomic DNA
X61122
EMBL· GenBank· DDBJ
CAA43434.3
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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