P24813 · AP2_YEAST

Function

function

Transcription activator involved in oxidative stress response and cadmium resistance. Regulates the transcription of genes overrepresented for the function of stabilizing proteins including the inducible Hsp90-family protein HSP82. Preferentially binds to promoters with the core binding site 5'-TTA[CG]TAA-3'. Activity of the transcription factor is controlled through oxidation of specific cysteine residues resulting in the alteration of its subcellular location. Activation by alkyl hydroperoxides or cadmium induces nuclear accumulation and as a result CAD1/YAP2 transcriptional activity.

Miscellaneous

One of 8 closely related fungi-specific YAP proteins (YAP1 to YAP8), which all seem to be transcription activators of the environmental stress response and metabolism control pathways and to have similar but not identical DNA binding specificities.
Present with 623 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentnucleus
Cellular ComponentRNA polymerase II transcription regulator complex
Molecular FunctionDNA-binding transcription activator activity, RNA polymerase II-specific
Molecular Functiontranscription cis-regulatory region binding
Biological Processcellular response to stress
Biological Processpositive regulation of transcription by RNA polymerase II
Biological Processresponse to cadmium ion

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    AP-1-like transcription factor YAP2
  • Alternative names
    • Cadmium resistance protein 1
    • Transcription factor CAD1

Gene names

    • Name
      CAD1
    • Synonyms
      YAP2
    • ORF names
      D9461.12
    • Ordered locus names
      YDR423C

Organism names

Accessions

  • Primary accession
    P24813
  • Secondary accessions
    • D6VT53
    • Q02259

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm
Nucleus
Note: Oxidized CAD1/YAP2 is found predominantly in the nucleus, while reduced CAD1/YAP2 is continuously exported to the cytoplasm by CRM1/exportin 1.

Keywords

Phenotypes & Variants

Disruption phenotype

Hypersensitive to the cytotoxic metal cadmium.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis356Only partially accumulates in the nucleus in response to cadmium. Does not accumulate in the nucleus; when associated with A-387.
Mutagenesis378No effect.
Mutagenesis387Only partially accumulates in the nucleus in response to cadmium. Does not accumulate in the nucleus; when associated with A-356.
Mutagenesis391Does not accumulate in the nucleus in response to cadmium.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 19 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

Miscellaneous

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000765221-409AP-1-like transcription factor YAP2

Post-translational modification

Depending on the oxidative stress inducing agent, CAD1/YAP2 can undergo two distinct conformational changes, both through oxidation of cysteine residues, and both masking the nuclear export signal, thus abolishing nuclear export by CRM1/exportin 1. Peroxide stress induces the formation of possible intramolecular disulfide bonds as well as intermolcular disulfide within a homodimer. Cadmium may bind directly to specific cysteine residues (Cys-391 and either Cys-356 or Cys-387) in the c-CRD.

Keywords

Proteomic databases

PTM databases

Expression

Induction

CAD1/YAP2 expression is at least partially regulated at the level of mRNA stability. Two small upstream open reading frames (uORF) in its mRNA cause increased RNA decay. The translation initiation factor eIF2 counteracts this effect by causing reinitiation at the functional initiation site, thus suppressing RNA decay.

Interaction

Subunit

Homodimer; disulfide-linked, upon oxidation (Probable). Interacts in the nucleus with the nuclear export protein CRM1 (PubMed:17187783).
Interacts with RCK1 (PubMed:15341652).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for motif, region, domain, compositional bias.

Type
IDPosition(s)Description
Motif17-24Bipartite nuclear localization signal
Region26-64Disordered
Domain43-106bZIP
Region46-69Basic motif
Motif47-54Bipartite nuclear localization signal
Region71-99Leucine-zipper
Region127-156Disordered
Compositional bias136-156Polar residues
Region356-387c-CRD
Motif372-379Nuclear export signal

Domain

Contains a C-terminal cysteine rich domain (c-CRD), but lacks the N-terminal CRD (n-CRD) found in its paralog YAP1. It probably also contains embedded in the c-CRD a nuclear export signal, with which the nuclear export protein CRM1/exportin 1 may interact in the absence of inter- or intramolecular disulfide bonds (or otherwise oxidized/modified cysteines) within the c-CRD.

Sequence similarities

Belongs to the bZIP family. YAP subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    409
  • Mass (Da)
    45,752
  • Last updated
    1993-10-01 v2
  • MD5 Checksum
    5658031C2E8D742C9C2F3385C672D6E2
MGNILRKGQQIYLAGDMKKQMLLNKDGTPKRKVGRPGRKRIDSEAKSRRTAQNRAAQRAFRDRKEAKMKSLQERVELLEQKDAQNKTTTDFLLCSLKSLLSEITKYRAKNSDDERILAFLDDLQEQQKRENEKGTSTAVSKAAKELPSPNSDENMTVNTSIEVQPHTQENEKVMWNIGSWNAPSLTNSWDSPPGNRTGAVTIGDESINGSEMPDFSLDLVSNDRQTGLEALDYDIHNYFPQHSERLTAEKIDTSACQCEIDQKYLPYETEDDTLFPSVLPLAVGSQCNNICNRKCIGTKPCSNKEIKCDLITSHLLNQKSLASVLPVAASHTKTIRTQSEAIEHISSAISNGKASCYHILEEISSLPKYSSLDIDDLCSELIIKAKCTDDCKIVVKARDLQSALVRQLL

Sequence caution

The sequence AAA34463.1 differs from that shown. Reason: Frameshift

Features

Showing features for sequence conflict, compositional bias.

Type
IDPosition(s)Description
Sequence conflict36in Ref. 6; AAA34463
Sequence conflict90in Ref. 6; AAA34463
Sequence conflict102in Ref. 6; AAA34463
Sequence conflict125in Ref. 6; AAA34463
Sequence conflict131in Ref. 6; AAA34463
Compositional bias136-156Polar residues
Sequence conflict142in Ref. 6; AAA34463
Sequence conflict145in Ref. 6; AAA34463
Sequence conflict327in Ref. 3; AAB29937
Sequence conflict337in Ref. 3; AAB29937
Sequence conflict347in Ref. 3; AAB29937
Sequence conflict354in Ref. 3; AAB29937

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L14289
EMBL· GenBank· DDBJ
AAA02920.1
EMBL· GenBank· DDBJ
Unassigned DNA
X69106
EMBL· GenBank· DDBJ
CAA48858.1
EMBL· GenBank· DDBJ
mRNA
S68847
EMBL· GenBank· DDBJ
AAB29937.1
EMBL· GenBank· DDBJ
Genomic DNA
U33007
EMBL· GenBank· DDBJ
AAB64878.1
EMBL· GenBank· DDBJ
Genomic DNA
M58331
EMBL· GenBank· DDBJ
AAA34463.1
EMBL· GenBank· DDBJ
Genomic DNA Frameshift
BK006938
EMBL· GenBank· DDBJ
DAA12263.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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