P24813 · AP2_YEAST
- ProteinAP-1-like transcription factor YAP2
- GeneCAD1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids409 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription activator involved in oxidative stress response and cadmium resistance. Regulates the transcription of genes overrepresented for the function of stabilizing proteins including the inducible Hsp90-family protein HSP82. Preferentially binds to promoters with the core binding site 5'-TTA[CG]TAA-3'. Activity of the transcription factor is controlled through oxidation of specific cysteine residues resulting in the alteration of its subcellular location. Activation by alkyl hydroperoxides or cadmium induces nuclear accumulation and as a result CAD1/YAP2 transcriptional activity.
Miscellaneous
One of 8 closely related fungi-specific YAP proteins (YAP1 to YAP8), which all seem to be transcription activators of the environmental stress response and metabolism control pathways and to have similar but not identical DNA binding specificities.
Present with 623 molecules/cell in log phase SD medium.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Cellular Component | RNA polymerase II transcription regulator complex | |
Molecular Function | DNA-binding transcription activator activity, RNA polymerase II-specific | |
Molecular Function | transcription cis-regulatory region binding | |
Biological Process | cellular response to stress | |
Biological Process | positive regulation of transcription by RNA polymerase II | |
Biological Process | response to cadmium ion |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAP-1-like transcription factor YAP2
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP24813
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
Hypersensitive to the cytotoxic metal cadmium.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 356 | Only partially accumulates in the nucleus in response to cadmium. Does not accumulate in the nucleus; when associated with A-387. | |||
Mutagenesis | 378 | No effect. | |||
Mutagenesis | 387 | Only partially accumulates in the nucleus in response to cadmium. Does not accumulate in the nucleus; when associated with A-356. | |||
Mutagenesis | 391 | Does not accumulate in the nucleus in response to cadmium. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 19 variants from UniProt as well as other sources including ClinVar and dbSNP.
Miscellaneous
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000076522 | 1-409 | AP-1-like transcription factor YAP2 | ||
Post-translational modification
Depending on the oxidative stress inducing agent, CAD1/YAP2 can undergo two distinct conformational changes, both through oxidation of cysteine residues, and both masking the nuclear export signal, thus abolishing nuclear export by CRM1/exportin 1. Peroxide stress induces the formation of possible intramolecular disulfide bonds as well as intermolcular disulfide within a homodimer. Cadmium may bind directly to specific cysteine residues (Cys-391 and either Cys-356 or Cys-387) in the c-CRD.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Induction
CAD1/YAP2 expression is at least partially regulated at the level of mRNA stability. Two small upstream open reading frames (uORF) in its mRNA cause increased RNA decay. The translation initiation factor eIF2 counteracts this effect by causing reinitiation at the functional initiation site, thus suppressing RNA decay.
Structure
Family & Domains
Features
Showing features for motif, region, domain, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 17-24 | Bipartite nuclear localization signal | |||
Region | 26-64 | Disordered | |||
Domain | 43-106 | bZIP | |||
Region | 46-69 | Basic motif | |||
Motif | 47-54 | Bipartite nuclear localization signal | |||
Region | 71-99 | Leucine-zipper | |||
Region | 127-156 | Disordered | |||
Compositional bias | 136-156 | Polar residues | |||
Region | 356-387 | c-CRD | |||
Motif | 372-379 | Nuclear export signal | |||
Domain
Contains a C-terminal cysteine rich domain (c-CRD), but lacks the N-terminal CRD (n-CRD) found in its paralog YAP1. It probably also contains embedded in the c-CRD a nuclear export signal, with which the nuclear export protein CRM1/exportin 1 may interact in the absence of inter- or intramolecular disulfide bonds (or otherwise oxidized/modified cysteines) within the c-CRD.
Sequence similarities
Belongs to the bZIP family. YAP subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length409
- Mass (Da)45,752
- Last updated1993-10-01 v2
- MD5 Checksum5658031C2E8D742C9C2F3385C672D6E2
Sequence caution
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 36 | in Ref. 6; AAA34463 | |||
Sequence conflict | 90 | in Ref. 6; AAA34463 | |||
Sequence conflict | 102 | in Ref. 6; AAA34463 | |||
Sequence conflict | 125 | in Ref. 6; AAA34463 | |||
Sequence conflict | 131 | in Ref. 6; AAA34463 | |||
Compositional bias | 136-156 | Polar residues | |||
Sequence conflict | 142 | in Ref. 6; AAA34463 | |||
Sequence conflict | 145 | in Ref. 6; AAA34463 | |||
Sequence conflict | 327 | in Ref. 3; AAB29937 | |||
Sequence conflict | 337 | in Ref. 3; AAB29937 | |||
Sequence conflict | 347 | in Ref. 3; AAB29937 | |||
Sequence conflict | 354 | in Ref. 3; AAB29937 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L14289 EMBL· GenBank· DDBJ | AAA02920.1 EMBL· GenBank· DDBJ | Unassigned DNA | ||
X69106 EMBL· GenBank· DDBJ | CAA48858.1 EMBL· GenBank· DDBJ | mRNA | ||
S68847 EMBL· GenBank· DDBJ | AAB29937.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U33007 EMBL· GenBank· DDBJ | AAB64878.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M58331 EMBL· GenBank· DDBJ | AAA34463.1 EMBL· GenBank· DDBJ | Genomic DNA | Frameshift | |
BK006938 EMBL· GenBank· DDBJ | DAA12263.1 EMBL· GenBank· DDBJ | Genomic DNA |