P24670 · AROD_SALTI

  • Protein
    3-dehydroquinate dehydratase
  • Gene
    aroD
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate. The reaction involves the formation of an imine intermediate between the keto group of 3-dehydroquinate and the epsilon-amino group of Lys-170 at the active site.

Catalytic activity

Activity regulation

Inhibited by (2R)-2-methyl-3-dehydroquinic acid.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
24 μM3-dehydroquinate7.225
kcat is 1.1 sec-1 for dehydratase activity with 3-dehydroquinate (at pH 7.2 and 25 degrees Celsius).

Pathway

Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site213-dehydroquinate (UniProtKB | ChEBI)
Binding site46-483-dehydroquinate (UniProtKB | ChEBI)
Binding site823-dehydroquinate (UniProtKB | ChEBI)
Active site143Proton donor/acceptor
Active site170Schiff-base intermediate with substrate
Binding site2133-dehydroquinate (UniProtKB | ChEBI)
Binding site2323-dehydroquinate (UniProtKB | ChEBI)
Binding site2363-dehydroquinate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function3-dehydroquinate dehydratase activity
Biological Process3,4-dihydroxybenzoate biosynthetic process
Biological Processamino acid biosynthetic process
Biological Processaromatic amino acid family biosynthetic process
Biological Processchorismate biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-dehydroquinate dehydratase
  • EC number
  • Short names
    3-dehydroquinase
  • Alternative names
    • Type I DHQase
    • Type I dehydroquinase
      (DHQ1
      )

Gene names

    • Name
      aroD
    • Ordered locus names
      STY1760, t1231

Organism names

  • Taxonomic identifier
  • Strains
    • ATCC 700931 / Ty2
    • CT18
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Salmonella

Accessions

  • Primary accession
    P24670

Proteomes

Phenotypes & Variants

Chemistry

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001388051-2523-dehydroquinate dehydratase

Interaction

Subunit

Dimer of dimers.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the type-I 3-dehydroquinase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    252
  • Mass (Da)
    27,649
  • Last updated
    2001-12-05 v2
  • Checksum
    F9D4D4C86D18F17B
MKTVTVKNLIIGEGMPKIIVSLMGRDINSVKAEALAYREATFDILEWRVDHFMDIASTQSVLTAARVIRDAMPDIPLLFTFRSAKEGGEQTITTQHYLTLNRAAIDSGLVDMIDLELFTGDADVKATVDYAHAHNVYVVMSNHDFHQTPSAEEMVLRLRKMQALGADIPKIAVMPQSKHDVLTLLTATLEMQQHYADRPVITMSMAKEGVISRLAGEVFGSAATFGAVKQASAPGQIAVNDLRSVLMILHNA

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict156in Ref. 1; CAA38418

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X54546
EMBL· GenBank· DDBJ
CAA38418.1
EMBL· GenBank· DDBJ
Genomic DNA
AL513382
EMBL· GenBank· DDBJ
CAD02002.1
EMBL· GenBank· DDBJ
Genomic DNA
AE014613
EMBL· GenBank· DDBJ
AAO68886.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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