P24474 · NIRS_PSEAE
- ProteinNitrite reductase
- GenenirS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids568 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
Catalytic activity
- Fe(III)-[cytochrome c] + H2O + nitric oxide = Fe(II)-[cytochrome c] + 2 H+ + nitrite
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 heme c group covalently per subunit.
Note: Binds 1 heme d1 group per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 72 | heme c (UniProtKB | ChEBI); covalent | ||||
Sequence: C | ||||||
Binding site | 75 | heme c (UniProtKB | ChEBI); covalent | ||||
Sequence: C | ||||||
Binding site | 76 | Fe (UniProtKB | ChEBI) of heme c (UniProtKB | ChEBI); axial binding residue | ||||
Sequence: H | ||||||
Binding site | 96 | heme c (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 109 | heme c (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 113 | Fe (UniProtKB | ChEBI) of heme c (UniProtKB | ChEBI); axial binding residue | ||||
Sequence: M | ||||||
Binding site | 207 | Fe (UniProtKB | ChEBI) of heme d1 (UniProtKB | ChEBI); proximal binding residue | ||||
Sequence: H | ||||||
Binding site | 250 | heme d1 (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 251 | heme d1 (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 270 | heme d1 (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 397 | heme d1 (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 508 | heme d1 (UniProtKB | ChEBI) | ||||
Sequence: Q |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | periplasmic space | |
Molecular Function | electron transfer activity | |
Molecular Function | heme binding | |
Molecular Function | hydroxylamine reductase activity | |
Molecular Function | metal ion binding | |
Molecular Function | nitrite reductase (NO-forming) activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNitrite reductase
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionP24474
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Chemistry
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-25 | |||||
Sequence: MPFGKPLVGTLLASLTLLGLATAHA | ||||||
Chain | PRO_0000006576 | 26-568 | Nitrite reductase | |||
Sequence: KDDMKAAEQYQGAASAVDPAHVVRTNGAPDMSESEFNEAKQIYFQRCAGCHGVLRKGATGKPLTPDITQQRGQQYLEALITYGTPLGMPNWGSSGELSKEQITLMAKYIQHTPPQPPEWGMPEMRESWKVLVKPEDRPKKQLNDLDLPNLFSVTLRDAGQIALVDGDSKKIVKVIDTGYAVHISRMSASGRYLLVIGRDARIDMIDLWAKEPTKVAEIKIGIEARSVESSKFKGYEDRYTIAGAYWPPQFAIMDGETLEPKQIVSTRGMTVDTQTYHPEPRVAAIIASHEHPEFIVNVKETGKVLLVNYKDIDNLTVTSIGAAPFLHDGGWDSSHRYFMTAANNSNKVAVIDSKDRRLSALVDVGKTPHPGRGANFVHPKYGPVWSTSHLGDGSISLIGTDPKNHPQYAWKKVAELQGQGGGSLFIKTHPKSSHLYVDTTFNPDARISQSVAVFDLKNLDAKYQVLPIAEWADLGEGAKRVVQPEYNKRGDEVWFSVWNGKNDSSALVVVDDKTLKLKAVVKDPRLITPTGKFNVYNTQHDVY |
Proteomic databases
Interaction
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 26-54 | N-terminal tail | ||||
Sequence: KDDMKAAEQYQGAASAVDPAHVVRTNGAP | ||||||
Domain | 55-140 | Cytochrome c | ||||
Sequence: DMSESEFNEAKQIYFQRCAGCHGVLRKGATGKPLTPDITQQRGQQYLEALITYGTPLGMPNWGSSGELSKEQITLMAKYIQHTPPQ | ||||||
Region | 141-568 | D1-heme domain | ||||
Sequence: PPEWGMPEMRESWKVLVKPEDRPKKQLNDLDLPNLFSVTLRDAGQIALVDGDSKKIVKVIDTGYAVHISRMSASGRYLLVIGRDARIDMIDLWAKEPTKVAEIKIGIEARSVESSKFKGYEDRYTIAGAYWPPQFAIMDGETLEPKQIVSTRGMTVDTQTYHPEPRVAAIIASHEHPEFIVNVKETGKVLLVNYKDIDNLTVTSIGAAPFLHDGGWDSSHRYFMTAANNSNKVAVIDSKDRRLSALVDVGKTPHPGRGANFVHPKYGPVWSTSHLGDGSISLIGTDPKNHPQYAWKKVAELQGQGGGSLFIKTHPKSSHLYVDTTFNPDARISQSVAVFDLKNLDAKYQVLPIAEWADLGEGAKRVVQPEYNKRGDEVWFSVWNGKNDSSALVVVDDKTLKLKAVVKDPRLITPTGKFNVYNTQHDVY |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length568
- Mass (Da)62,653
- Last updated1992-03-01 v1
- Checksum20FAE9FFE9127D62
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X16452 EMBL· GenBank· DDBJ | CAA34471.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE004091 EMBL· GenBank· DDBJ | AAG03908.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X51631 EMBL· GenBank· DDBJ | CAA35957.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X51319 EMBL· GenBank· DDBJ | CAA35702.1 EMBL· GenBank· DDBJ | Genomic DNA |