P24374 · GVPJ1_HALSA

Function

function

Proteins GvpF to GvpM might be involved in nucleating gas vesicle formation (Probable) (PubMed:15126480, PubMed:24846741, PubMed:33281806, PubMed:34975818).
Mutagenesis of residues 13-61 shows that almost none of them can be substituted and still make gas vesicles (PubMed:34975818).
A minor component of the gas vesicle (Probable) (PubMed:15126480, PubMed:21158390).
Gas vesicles are hollow, gas filled proteinaceous nanostructures found in several microbial planktonic microorganisms. They allow positioning of halobacteria at the optimal depth for growth in the poorly aerated, shallow brine pools of their habitat (PubMed:33711860).
Expression of a 9.5 kb p-vac DNA fragment containing 2 divergently transcribed regions (gvpD-gvpE-gvpF-gvpG-gvpH-gvpI-gvpJ-gvpK-gvpL-gvpM and gvpA-gvpC-gvpN-gvpO) allows H.volcanii to produce gas vesicles. All site-directed mutagenesis is tested in H.volcanii (PubMed:10894744, PubMed:1404376, PubMed:7651141).
A minimal gas vesicle can be made in H.volcanii by gvpA1-gvpO1 plus gvpF1-gvpG1-gvpJ1-gvpK1-gvpL1-gvpM1; lack of enough GvpJ1 prevents formation (PubMed:10894744).
A similar region restores gas vesicle production in H.halobium without the p-vac locus, but it still has the c-vac locus (PubMed:1398080, PubMed:8002589).

Miscellaneous

Encoded in a 14-gene plasmid locus called p-vac which produces predominantly short, spindle-shaped gas vesicles during all stages of growth.

Caution

The protein sequence for residues 47-58 could come from either GvpJ1 or GvpJ2.

GO annotations

AspectTerm
Cellular Componentgas vesicle
Cellular Componentvesicle membrane
Molecular Functionstructural molecule activity

Names & Taxonomy

Protein names

  • Recommended name
    Gas vesicle protein J1
  • Short names
    GvpJ1

Gene names

    • Name
      gvpJ11
    • Synonyms
      gvpJ
      , p-gvpJ
    • Ordered locus names
      VNG_5022G
    • Name
      gvpJ1
    • Ordered locus names
      VNG_6022G

Encoded on

  • Plasmid pNRC100
  • Plasmid pNRC200
  • Plasmid pHH1

Organism names

Accessions

  • Primary accession
    P24374
  • Secondary accessions
    • Q9HI25

Proteomes

    • Identifier
    • Component
      Plasmid pNRC100, Plasmid pNRC200

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Deletion of this gene prevents gas vesicle formation.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis2-4Makes a few gas vesicles, still interacts with GvpL.
Mutagenesis2-5Does not make gas vesicles, still interacts with GvpL.
Mutagenesis13Does not make gas vesicles, still interacts with GvpL.
Mutagenesis13Very few gas vesicles, still interacts with GvpL.
Mutagenesis19Makes no gas vesicles, decreased interaction with GvpL.
Mutagenesis20Makes no gas vesicles, decreased interaction with GvpL.
Mutagenesis22Mix of cylindrical and spindle-shaped gas vesicles, still interacts with GvpL.
Mutagenesis23Unstable gas vesicles that do not float, still interacts with GvpL.
Mutagenesis24Makes no gas vesicles, decreased interaction with GvpL.
Mutagenesis25Unstable gas vesicles that do not float, still interacts with GvpL.
Mutagenesis39Very few gas vesicles, still interacts with GvpL.
Mutagenesis46Unstable gas vesicles that do not float, still interacts with GvpL.
Mutagenesis58Makes no gas vesicles, decreased interaction with GvpL.
Mutagenesis69Cylindrical gas vesicles, still interacts with GvpL.
Mutagenesis74Does not make gas vesicles, still interacts with GvpL.
Mutagenesis110-114Does not make gas vesicles, still interacts with GvpL.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002000031-114Gas vesicle protein J1

Expression

Induction

Probably part of a gvpF1-gvpG1-gvpH1-gvpI1-gvpJ1-gvpK1-gvpL1-gvpM1 operon, maximally expressed in early to mid log phase (Probable) (PubMed:1956294).
Gas vesicles appear earlier when grown in static culture, possibly due to O2-limitation (PubMed:33711860).

Interaction

Subunit

GvpF to GvpM interact with each other in vitro, and may form multi-subunit complex(es) (PubMed:24846741, PubMed:33281806, PubMed:34975818).
Interacts with GvpA1 (PubMed:34975818, PubMed:35966690).

Structure

Family & Domains

Features

Showing features for region, motif, compositional bias.

TypeIDPosition(s)Description
Region13-22Alpha helix 1
Region25-35Beta-strand 1
Region40-50Beta-strand 2
Motif46-50Conserved in GvpM1/2 but not GvpA
Region52-72Alpha helix 2
Region63-114Disordered
Compositional bias75-114Polar residues
Region78-87Alpha helix 3
Region95-105Alpha helix 4

Domain

Modeled by homology to GvpA to have 4 alpha helices and 2 beta-sheets. Extensive mutagenesis shows that most mutations in the N-terminal region (homologous to GvpA) do not make gas vesicles (PubMed:34975818).
A short motif conserved in GvpJ1/2 and GvpM1/2 (but not GvpA), which is predicted to be on the outer surface of the proteins, is important for the function of GvpJ and GvpM (PubMed:34975818).

Sequence similarities

Belongs to the gas vesicle GvpA family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    114
  • Mass (Da)
    11,983
  • Last updated
    1992-03-01 v1
  • Checksum
    DC4EB22F0D55FDC0
MSDPKPTRSQGDLAEMLEMLLDKGVVVNADIAVSVGDTELLGIELRAAIASFETAAEYGLEFPTGTDMERVESAANISPDQSDPASETQSETESTNPLSDDSTPTASTSAEETK

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias75-114Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M58557
EMBL· GenBank· DDBJ
AAA98189.1
EMBL· GenBank· DDBJ
Genomic DNA
X55648
EMBL· GenBank· DDBJ
CAA39177.1
EMBL· GenBank· DDBJ
Genomic DNA
AF016485
EMBL· GenBank· DDBJ
AAC82802.1
EMBL· GenBank· DDBJ
Genomic DNA
AE004438
EMBL· GenBank· DDBJ
AAG20719.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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