P24346 · DDX3_XENLA
- ProteinPutative ATP-dependent RNA helicase an3
- Genean3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids697 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Multifunctional ATP-dependent RNA helicase. The ATPase activity can be stimulated by various ribo-and deoxynucleic acids indicative for a relaxed substrate specificity. In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs. Involved in many cellular processes, which do not necessarily require its ATPase/helicase catalytic activities. Involved in the regulation of transcription and translation initiation. Involved in innate immunity (By similarity).
Involved in both stress and inflammatory responses (By similarity).
May negatively regulate extrinsic apoptotic signaling pathway via death domain receptors. May be involved in mitotic chromosome segregation (By similarity).
Required for canonical Wnt signaling involved in anteroposterior neural patterning (PubMed:23413191).
Involved in both stress and inflammatory responses (By similarity).
May negatively regulate extrinsic apoptotic signaling pathway via death domain receptors. May be involved in mitotic chromosome segregation (By similarity).
Required for canonical Wnt signaling involved in anteroposterior neural patterning (PubMed:23413191).
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | canonical inflammasome complex | |
Cellular Component | cell leading edge | |
Cellular Component | cytoplasm | |
Cellular Component | cytoplasmic stress granule | |
Cellular Component | lamellipodium | |
Cellular Component | nucleus | |
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA helicase activity | |
Biological Process | negative regulation of extrinsic apoptotic signaling pathway via death domain receptors |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePutative ATP-dependent RNA helicase an3
- EC number
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus
Accessions
- Primary accessionP24346
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Shuttles between the nucleus and the cytosol.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Morpholino knockdown of the protein causes anteriorization of embryos, that exhibit enlarged heads and eyes, shortened tails and defective melanocyte and eye pigmentation.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000055015 | 1-697 | Putative ATP-dependent RNA helicase an3 | |||
Sequence: MSHVAVENVLNLDQQFAGLDLNSADAESGVAGTKGRYIPPHLRNKEASRNDSNWDSGRGGNGYINGMQDDRDGRMNGYDRGGYGSRGTGRSDRGFYDRENSGWNSGRDKDAYSSFGSRGDRGKGSLFNERGSGSRRTDDRRQDGFDGMGNRSDKSGFGRFDRGNSRWSDDRNDEDDWSKPLAPNDRVEQELFSGSNTGINFEKYDDIPVEATGSNCPPHIESFHDVTMGEIIMGNIQLTRYTRPTPVQKHAIPIIIEKRDLMACAQTGSGKTAAFLLPILSQIYADGPGDAMKHLQENGRYGRRKQFPLSLVLAPTRELAVQIYEEARKFAYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRQTMMFSATFPKEIQILARDFLDEYIFLAVGRVGSTSENITQKVVWVEEMDKRSFLLDLLNATGKDSLTLVFVETKKGADALEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNEKNINITKDLLDLLVEAKQEVPSWLENMAYEQHHKSSSRGRSKSRFSGGFGAKDYRQSSGAGSSFGSSRGGRSSGHGGSRGFGGGYGGFYNSDGYGGNYGGSSQVDWWGN |
Expression
Developmental stage
Widely expressed throughout embryogenesis. At gastrula stage (st. 10.5), equal expression in ventral and dorsal marginal zones. At neurula stage (st. 18), highest expression levels in ectodermal cells.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 27-189 | Disordered | ||||
Sequence: ESGVAGTKGRYIPPHLRNKEASRNDSNWDSGRGGNGYINGMQDDRDGRMNGYDRGGYGSRGTGRSDRGFYDRENSGWNSGRDKDAYSSFGSRGDRGKGSLFNERGSGSRRTDDRRQDGFDGMGNRSDKSGFGRFDRGNSRWSDDRNDEDDWSKPLAPNDRVEQ | ||||||
Compositional bias | 91-105 | Basic and acidic residues | ||||
Sequence: SDRGFYDRENSGWNS | ||||||
Compositional bias | 132-148 | Basic and acidic residues | ||||
Sequence: SGSRRTDDRRQDGFDGM | ||||||
Compositional bias | 155-183 | Basic and acidic residues | ||||
Sequence: SGFGRFDRGNSRWSDDRNDEDDWSKPLAP | ||||||
Motif | 221-249 | Q motif | ||||
Sequence: ESFHDVTMGEIIMGNIQLTRYTRPTPVQK | ||||||
Domain | 252-444 | Helicase ATP-binding | ||||
Sequence: IPIIIEKRDLMACAQTGSGKTAAFLLPILSQIYADGPGDAMKHLQENGRYGRRKQFPLSLVLAPTRELAVQIYEEARKFAYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRQTMMFSATFPKEIQILARDFLDEYIFL | ||||||
Motif | 388-391 | DEAD box | ||||
Sequence: DEAD | ||||||
Domain | 455-616 | Helicase C-terminal | ||||
Sequence: NITQKVVWVEEMDKRSFLLDLLNATGKDSLTLVFVETKKGADALEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNEKNINITKDLLDLLVEAKQEVPSWLENMA | ||||||
Region | 619-666 | Disordered | ||||
Sequence: QHHKSSSRGRSKSRFSGGFGAKDYRQSSGAGSSFGSSRGGRSSGHGGS | ||||||
Compositional bias | 644-661 | Polar residues | ||||
Sequence: QSSGAGSSFGSSRGGRSS |
Sequence similarities
Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length697
- Mass (Da)77,303
- Last updated1992-03-01 v1
- ChecksumF3DD23EB60B2E2EF
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 91-105 | Basic and acidic residues | ||||
Sequence: SDRGFYDRENSGWNS | ||||||
Compositional bias | 132-148 | Basic and acidic residues | ||||
Sequence: SGSRRTDDRRQDGFDGM | ||||||
Compositional bias | 155-183 | Basic and acidic residues | ||||
Sequence: SGFGRFDRGNSRWSDDRNDEDDWSKPLAP | ||||||
Compositional bias | 644-661 | Polar residues | ||||
Sequence: QSSGAGSSFGSSRGGRSS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X57328 EMBL· GenBank· DDBJ | CAA40605.1 EMBL· GenBank· DDBJ | mRNA |