P24346 · DDX3_XENLA

Function

function

Multifunctional ATP-dependent RNA helicase. The ATPase activity can be stimulated by various ribo-and deoxynucleic acids indicative for a relaxed substrate specificity. In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs. Involved in many cellular processes, which do not necessarily require its ATPase/helicase catalytic activities. Involved in the regulation of transcription and translation initiation. Involved in innate immunity (By similarity).
Involved in both stress and inflammatory responses (By similarity).
May negatively regulate extrinsic apoptotic signaling pathway via death domain receptors. May be involved in mitotic chromosome segregation (By similarity).
Required for canonical Wnt signaling involved in anteroposterior neural patterning (PubMed:23413191).

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site241-248ATP (UniProtKB | ChEBI)
Binding site265-272ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcanonical inflammasome complex
Cellular Componentcell leading edge
Cellular Componentcytoplasm
Cellular Componentcytoplasmic stress granule
Cellular Componentlamellipodium
Cellular Componentnucleus
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionRNA binding
Molecular FunctionRNA helicase activity
Biological Processnegative regulation of extrinsic apoptotic signaling pathway via death domain receptors

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Putative ATP-dependent RNA helicase an3
  • EC number

Gene names

    • Name
      an3

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus

Accessions

  • Primary accession
    P24346

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Morpholino knockdown of the protein causes anteriorization of embryos, that exhibit enlarged heads and eyes, shortened tails and defective melanocyte and eye pigmentation.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000550151-697Putative ATP-dependent RNA helicase an3

Expression

Developmental stage

Widely expressed throughout embryogenesis. At gastrula stage (st. 10.5), equal expression in ventral and dorsal marginal zones. At neurula stage (st. 18), highest expression levels in ectodermal cells.

Gene expression databases

    • 397935Expressed in pancreas and 19 other cell types or tissues

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, compositional bias, motif, domain.

TypeIDPosition(s)Description
Region27-189Disordered
Compositional bias91-105Basic and acidic residues
Compositional bias132-148Basic and acidic residues
Compositional bias155-183Basic and acidic residues
Motif221-249Q motif
Domain252-444Helicase ATP-binding
Motif388-391DEAD box
Domain455-616Helicase C-terminal
Region619-666Disordered
Compositional bias644-661Polar residues

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    697
  • Mass (Da)
    77,303
  • Last updated
    1992-03-01 v1
  • Checksum
    F3DD23EB60B2E2EF
MSHVAVENVLNLDQQFAGLDLNSADAESGVAGTKGRYIPPHLRNKEASRNDSNWDSGRGGNGYINGMQDDRDGRMNGYDRGGYGSRGTGRSDRGFYDRENSGWNSGRDKDAYSSFGSRGDRGKGSLFNERGSGSRRTDDRRQDGFDGMGNRSDKSGFGRFDRGNSRWSDDRNDEDDWSKPLAPNDRVEQELFSGSNTGINFEKYDDIPVEATGSNCPPHIESFHDVTMGEIIMGNIQLTRYTRPTPVQKHAIPIIIEKRDLMACAQTGSGKTAAFLLPILSQIYADGPGDAMKHLQENGRYGRRKQFPLSLVLAPTRELAVQIYEEARKFAYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRQTMMFSATFPKEIQILARDFLDEYIFLAVGRVGSTSENITQKVVWVEEMDKRSFLLDLLNATGKDSLTLVFVETKKGADALEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNEKNINITKDLLDLLVEAKQEVPSWLENMAYEQHHKSSSRGRSKSRFSGGFGAKDYRQSSGAGSSFGSSRGGRSSGHGGSRGFGGGYGGFYNSDGYGGNYGGSSQVDWWGN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias91-105Basic and acidic residues
Compositional bias132-148Basic and acidic residues
Compositional bias155-183Basic and acidic residues
Compositional bias644-661Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X57328
EMBL· GenBank· DDBJ
CAA40605.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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