P24335 · WAG13_TROWA

Function

function

Waglerin-1 selectively blocks the epsilon subunit of muscle nicotinic acetylcholine receptor (nAChR). Has also effects on rodent ionotropic GABA(A) receptors (GABR), since it potentiates I(GABA) in some neurons and depresses I(GABA) in others. In mice, it elicits tachypnea, ocular proptosis, rapid collapse and spasms, whereas no toxic effects on respiration and blood pressure are observed in rats.
Waglerin-3 selectively blocks the epsilon subunit of muscle nicotinic acetylcholine receptor (nAChR). It elicits tachypnea, ocular proptosis, rapid collapse and spasms in mice. It causes death by respiratory failure.

Miscellaneous

Exists in two forms, due to cis-trans isomerization at 13-Pro-Pro-14.

Caution

Mutagenesis results from PubMed:8858103 should be interpreted carefully, since the authors found waglerin-2 (AC P58930) as inactive, despite the finding of other groups that found this peptide equipotent in lethality to waglerin-1.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Molecular Functionacetylcholine receptor inhibitor activity
Molecular Functionion channel regulator activity
Molecular Functiontoxin activity

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Waglerin-3
  • Alternative names
    • SL-Waglerin-1 (SL-I)
  • Cleaved into 1 chains
    • Waglerin-1 Alternative names: Lethal peptide I, Waglerin I, Wtx-1

Organism names

Accessions

  • Primary accession
    P24335

Subcellular Location

Keywords

Phenotypes & Variants

Toxic dose

LD50 of waglerin-1 is 0.33-0.369 mg/kg by intraperitoneal injection into mice.
LD50 of waglerin-3 is 0.22 mg/kg by intraperitoneal injection into mice.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis3-4No loss of affinity; in Waglerin-1.
Mutagenesis5No decrease in lethality; in Waglerin-1.
Mutagenesis7Slight decrease in lethality; in Waglerin-1.
Mutagenesis9Slight decrease in lethality; in Waglerin-1.
Mutagenesis113000-fold decrease in affinity at the alpha-epsilon site of nAChR and 4- to 9-fold decrease at the alpha-gamma and alpha-delta sites, respectively; when associated with S-15 in Waglerin-1.
Mutagenesis12Very important (34-fold) decrease in lethality; in Waglerin-1.
Mutagenesis12260-fold decrease in affinity; in Waglerin-1.
Mutagenesis153000-fold decrease in affinity at the alpha-epsilon site of nAChR and 4- to 9-fold decrease at the alpha-gamma and alpha-delta sites, respectively; when associated with S-11 in Waglerin-1.
Mutagenesis16Important (16-fold) decrease in lethality; in Waglerin-1.
Mutagenesis161160-fold decrease in affinity; in Waglerin-1.
Mutagenesis20Slight decrease in lethality; in Waglerin-1.
Mutagenesis22Slight decrease in lethality; in Waglerin-1.
Mutagenesis23-24No loss of affinity; in Waglerin-1.
Mutagenesis24Slight decrease in lethality; in Waglerin-1.

PTM/Processing

Features

Showing features for peptide, disulfide bond.

Type
IDPosition(s)Description
PeptidePRO_00004040851-24Waglerin-3
PeptidePRO_00000445493-24Waglerin-1
Disulfide bond11↔15

Post-translational modification

Amidation of the waglerin-1 C-terminus increases the affinity by 2-fold.

Keywords

Expression

Tissue specificity

Expressed by the venom gland.

Interaction

Subunit

Waglerin-1 is monomeric.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-24Disordered

Sequence similarities

Belongs to the waglerin family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    24
  • Mass (Da)
    2,722
  • Last updated
    2011-02-08 v2
  • Checksum
    D9B533A32692594F
SLGGKPDLRPCHPPCHYIPRPKPR

Keywords

Sequence databases

Similar Proteins

Disclaimer

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