P24197 · YGID_ECOLI
- Protein4,5-DOPA dioxygenase extradiol
- GeneygiD
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids271 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
In vitro, opens the cyclic ring of dihydroxy-phenylalanine (DOPA) between carbons 4 and 5, thus producing an unstable seco-DOPA that rearranges nonenzymatically to betalamic acid. The physiological substrate is unknown.
Miscellaneous
Betalamic acid is the structural unit of the betalains, natural nitrogen-containing water-soluble pigments with high colorant and bioactive properties, characteristic of plants of the order Caryophyllales.
Catalytic activity
- L-dopa + O2 = 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde + H+
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
7.9 mM | L-DOPA |
kcat is 0.17 min-1 with L-DOPA.
pH Dependence
Optimum pH is 8.0.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | DOPA dioxygenase activity | |
Molecular Function | ferrous iron binding | |
Molecular Function | stizolobate synthase activity | |
Molecular Function | zinc ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name4,5-DOPA dioxygenase extradiol
- EC number
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP24197
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000169405 | 1-271 | 4,5-DOPA dioxygenase extradiol | |||
Sequence: MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTLSNPTPEHYLPLLYVLGAWDGQEPITIPVEGIEMGSLSMLSVQIG |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length271
- Mass (Da)29,903
- Last updated2016-11-02 v4
- ChecksumA74E9035BAF6EB0F
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 243-270 | in Ref. 1; AAA71877 | ||||
Sequence: GAWDGQEPITIPVEGIEMGSLSMLSVQI → RYVGWAGANYHS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M77129 EMBL· GenBank· DDBJ | AAA71877.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U28377 EMBL· GenBank· DDBJ | AAA69207.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC76075.3 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE77095.1 EMBL· GenBank· DDBJ | Genomic DNA |