P24100 · CDKA1_ARATH

Function

function

Involved in the control of the cell cycle. Essential for both G1/S and G2/M (mitosis) phase transitions. Functions in cell morphogenesis as well as cell proliferation. Required for cell division (entry into mitosis) of the generative cell in male gametogenesis. Required to trigger guard mother cells (GMC) symmetric divisions at the late stage of stomatal development, probably via the regulation of G1 to S transition in the cell cycle. Required for the function of SPCH in entering the stomatal lineage (PubMed:25680231).
Promotes divisions in the guard cells (GCs) after the guard mother cells (GMC) symmetric division when in the presence of CYCD3-2 via the phosphorylation of SPCH (PubMed:24687979, PubMed:25680231).

Catalytic activity

Activity regulation

CDK kinase activated by CDKF-1. CDK kinase activity inhibited by KRP1/ICK1, KRP2/ICK2, KRP3/ICK6, KRP4/ICK7, KRP5/ICK3, KRP6/ICK4 and KRP7/ICK5. Down-regulated by phosphorylation by WEE1.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site10-18ATP (UniProtKB | ChEBI)
Binding site33ATP (UniProtKB | ChEBI)
Active site127Proton acceptor

GO annotations

AspectTerm
Cellular Componentcortical microtubule, transverse to long axis
Cellular Componentcytoplasm
Cellular Componentnucleus
Cellular Componentpreprophase band
Molecular FunctionATP binding
Molecular Functioncyclin-dependent protein serine/threonine kinase activity
Molecular Functionkinase activity
Molecular Functionprotein kinase activity
Molecular Functionprotein serine kinase activity
Molecular FunctionRNA polymerase II CTD heptapeptide repeat kinase activity
Biological Processasymmetric cell division
Biological Processcytokinesis by cell plate formation
Biological ProcessDNA endoreduplication
Biological Processembryo development ending in seed dormancy
Biological Processgametophyte development
Biological Processguard mother cell cytokinesis
Biological Processguard mother cell differentiation
Biological Processmeiotic cytokinesis
Biological Processpollen development
Biological Processpositive regulation of cell population proliferation
Biological Processregulation of cell cycle G1/S phase transition
Biological Processregulation of meiotic nuclear division
Biological Processresponse to cold
Biological Processsymmetric cell division

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cyclin-dependent kinase A-1
  • EC number
  • Short names
    CDKA;1
  • Alternative names
    • Cell division control protein 2 homolog A
      (CDC2aAt
      )

Gene names

    • Name
      CDKA-1
    • Synonyms
      CDC2
      , CDC2A
    • ORF names
      T21J18.20
    • Ordered locus names
      At3g48750

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    P24100
  • Secondary accessions
    • Q29Q50
    • Q8RX68
    • Q9M307

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Note: Mainly cytoplasmic. Nuclear distribution increases after binding to ICK1/KRP1.

Keywords

Phenotypes & Variants

Disruption phenotype

Plants display lethal male gametophyte (PubMed:16460514, PubMed:25680231).
Impaired stomata formation with arrested guard mother cells (GMC) divisions (PubMed:25680231).
Impaired last mitotic division in the male gametophyte, leading to 50 percent of pollen with two gametes (PubMed:25680231).
The double mutant flp-1 myb88 displays an enhanced stomatal phenotype with more and larger stomatal clusters. Triple mutants cdka;1 flp-1 myb88 don't have guard cells stacks but accumulates sGCs (PubMed:24687979).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis14Increased kinase activity; when associated with F-15.
Mutagenesis15Abolishes phosphorylation by WEE1. Increased kinase activity; when associated with A-14.
Mutagenesis146Decreased kinase activity and disturbed cell cycle. Reduced frequency of cell division during embryo development. Altered stomatal production. Interacts with SPCH.
Mutagenesis156Decreased kinase activity and disturbed cell cycle.
Mutagenesis161Strong reduction in kinase activity and ability to bind substrate. Strong reduction in plant growth. Sterile plants.
Mutagenesis161Strong reduction in kinase activity and ability to bind substrate.
Mutagenesis166Decreased kinase activity and disturbed cell cycle.
Mutagenesis234-236No change in kinase activity, but disturbed cell cycle. Loss of interaction with CKS1.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 3 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000857491-294Cyclin-dependent kinase A-1
Modified residue15Phosphotyrosine
Modified residue161Phosphothreonine

Post-translational modification

Phosphorylated at Tyr-15 by WEE1. Phosphorylation at Thr-161 is important for the kinase activity and substrate binding. Binding to the anti-phosphatase PAS2 prevents dephosphorylation.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in roots, stems, flowers and siliques.

Induction

By nematode infection in roots. Down-regulated by salt stress in root meristem and replication blocking agents (hydroxyurea and aphidicolin).

Developmental stage

Expressed throughout the cell cycle. Expressed in actively dividing cells: root and shoot apical meristems, leaf primordia and emerging lateral root meristem. Expressed in light-grown seedlings from 1 up to 7 days after germination with a peak at 2 and 3 days.

Gene expression databases

Interaction

Subunit

Interacts with CDT1A, CYCA2-3, CYCD2-1, CYCD3-1, CYCD4-1, CYCD4-2, CYCH1-1, CYCU1-1, CYCU2-1, CYCU2-2, CYCU3-1, CYCU4-1, CYCU4-2, CYCU4-3, CKS1, KRP2/ICK2, KRP3/ICK6, KRP4/ICK7, KRP6/ICK4, KRP7/ICK5, and C-terminal domain of KRP1/ICK1. Interacts with WEE1 and TIF4A-1/EIF4A-1. Interacts with PAS2; when phosphorylated at Tyr-15. Interacts with SMR3, SMR4, SMR5, SMR6, SMR8 and At4g14310. Binds to CYCD3-2 (PubMed:24687979).
Component of a DREAM-like complex which modulates a variety of developmentally regulated genes and of the mitotic genes in proliferating and differentiated cells. Interacts with MYB3R3 at later and with MYB3R4 at earlier stages of leaf development (PubMed:26069325).
May interact with SPCH (PubMed:25680231).

Binary interactions

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain4-287Protein kinase

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    294
  • Mass (Da)
    34,030
  • Last updated
    1992-03-01 v1
  • Checksum
    B5FAE55FA9EC366E
MDQYEKVEKIGEGTYGVVYKARDKVTNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHSNIVKLQDVVHSEKRLYLVFEYLDLDLKKHMDSTPDFSKDLHMIKTYLYQILRGIAYCHSHRVLHRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGSHHYSTPVDIWSVGCIFAEMISQKPLFPGDSEIDQLFKIFRIMGTPYEDTWRGVTSLPDYKSAFPKWKPTDLETFVPNLDPDGVDLLSKMLLMDPTKRINARAALEHEYFKDLGGMP

Sequence caution

The sequence AAL91258.1 differs from that shown. Reason: Frameshift
The sequence CAB87903.1 differs from that shown. Reason: Erroneous gene model prediction

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M59198
EMBL· GenBank· DDBJ
AAA32831.1
EMBL· GenBank· DDBJ
mRNA
S45387
EMBL· GenBank· DDBJ
AAB23643.1
EMBL· GenBank· DDBJ
mRNA
X57839
EMBL· GenBank· DDBJ
CAA40971.1
EMBL· GenBank· DDBJ
mRNA
D10850
EMBL· GenBank· DDBJ
BAA01623.1
EMBL· GenBank· DDBJ
Genomic DNA
AL132963
EMBL· GenBank· DDBJ
CAB87903.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
CP002686
EMBL· GenBank· DDBJ
AEE78452.1
EMBL· GenBank· DDBJ
Genomic DNA
AY090353
EMBL· GenBank· DDBJ
AAL91258.1
EMBL· GenBank· DDBJ
mRNA Frameshift
BT024706
EMBL· GenBank· DDBJ
ABD59044.1
EMBL· GenBank· DDBJ
mRNA
AK226373
EMBL· GenBank· DDBJ
BAE98520.1
EMBL· GenBank· DDBJ
mRNA
AY085153
EMBL· GenBank· DDBJ
AAM61706.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp