P23634 · AT2B4_HUMAN
- ProteinPlasma membrane calcium-transporting ATPase 4
- GeneATP2B4
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids1241 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
By regulating sperm cell calcium homeostasis, may play a role in sperm motility (By similarity).
Catalytic activity
- ATP + Ca2+(in) + H2O = ADP + Ca2+(out) + H+ + phosphate
CHEBI:30616 + Ca2+ (in)CHEBI:29108+ CHEBI:15377 = CHEBI:456216 + Ca2+ (out)CHEBI:29108+ CHEBI:15378 + CHEBI:43474
Activity regulation
Features
Showing features for active site, binding site.
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePlasma membrane calcium-transporting ATPase 4
- EC number
- Short namesPMCA4
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Hominidae > Homo
Accessions
- Primary accessionP23634
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-92 | Cytoplasmic | ||||
Sequence: MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQINVHYGGVQNLCSRLKTSPVEGLSGNPADLEKRRQVFGHNVIPPKKPKT | ||||||
Transmembrane | 93-113 | Helical | ||||
Sequence: FLELVWEALQDVTLIILEIAA | ||||||
Topological domain | 114-150 | Extracellular | ||||
Sequence: IISLVLSFYRPAGEENELCGQVATTPEDENEAQAGWI | ||||||
Transmembrane | 151-171 | Helical | ||||
Sequence: EGAAILFSVIIVVLVTAFNDW | ||||||
Topological domain | 172-356 | Cytoplasmic | ||||
Sequence: SKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDNEEKDKKAVKVPKKEKSVLQGKL | ||||||
Transmembrane | 357-376 | Helical | ||||
Sequence: TRLAVQIGKAGLLMSALTVF | ||||||
Topological domain | 377-409 | Extracellular | ||||
Sequence: ILILYFVIDNFVINRRPWLPECTPIYIQYFVKF | ||||||
Transmembrane | 410-427 | Helical | ||||
Sequence: FIIGITVLVVAVPEGLPL | ||||||
Topological domain | 428-840 | Cytoplasmic | ||||
Sequence: AVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQIPSPDVFLPKVLDLIVNGISINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEEKLYKVYTFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVRTVIEPMACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAIAKCKQAGITVRMVTGDNINTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTVGEHRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSI | ||||||
Transmembrane | 841-860 | Helical | ||||
Sequence: SKFLQFQLTVNVVAVIVAFT | ||||||
Topological domain | 861-870 | Extracellular | ||||
Sequence: GACITQDSPL | ||||||
Transmembrane | 871-891 | Helical | ||||
Sequence: KAVQMLWVNLIMDTFASLALA | ||||||
Topological domain | 892-911 | Cytoplasmic | ||||
Sequence: TEPPTESLLKRRPYGRNKPL | ||||||
Transmembrane | 912-934 | Helical | ||||
Sequence: ISRTMMKNILGHAFYQLIVIFIL | ||||||
Topological domain | 935-952 | Extracellular | ||||
Sequence: VFAGEKFFDIDSGRKAPL | ||||||
Transmembrane | 953-974 | Helical | ||||
Sequence: HSPPSQHYTIVFNTFVLMQLFN | ||||||
Topological domain | 975-993 | Cytoplasmic | ||||
Sequence: EINSRKIHGEKNVFSGIYR | ||||||
Transmembrane | 994-1015 | Helical | ||||
Sequence: NIIFCSVVLGTFICQIFIVEFG | ||||||
Topological domain | 1016-1025 | Extracellular | ||||
Sequence: GKPFSCTSLS | ||||||
Transmembrane | 1026-1047 | Helical | ||||
Sequence: LSQWLWCLFIGIGELLWGQFIS | ||||||
Topological domain | 1048-1241 | Cytoplasmic | ||||
Sequence: AIPTRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRRGQILWFRGLNRIQTQIDVINTFQTGASFKGVLRRQNMGQHLDVKLVPSSSYIKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETSV |
Keywords
- Cellular component
Disease & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 672 | Strongly decreased calcium transport activity. Slowed decomposition of the phosphorylated intermediate. | ||||
Sequence: D → Q | ||||||
Mutagenesis | 674 | Decreased calcium transport activity. | ||||
Sequence: V → P | ||||||
Mutagenesis | 675 | Decreased calcium transport activity. | ||||
Sequence: R → K, D, or L | ||||||
Mutagenesis | 686 | Decreased calcium transport activity. | ||||
Sequence: K → L | ||||||
Mutagenesis | 693 | Mildly decreased calcium transport activity. | ||||
Sequence: R → I |
Organism-specific databases
Miscellaneous
Chemistry
Genetic variation databases
PTM/Processing
Features
Showing features for chain, modified residue, modified residue (large scale data).
Type | ID | Position(s) | Source | Description | |||
---|---|---|---|---|---|---|---|
Chain | PRO_0000046220 | 1-1241 | UniProt | Plasma membrane calcium-transporting ATPase 4 | |||
Sequence: MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQINVHYGGVQNLCSRLKTSPVEGLSGNPADLEKRRQVFGHNVIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPAGEENELCGQVATTPEDENEAQAGWIEGAAILFSVIIVVLVTAFNDWSKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDNEEKDKKAVKVPKKEKSVLQGKLTRLAVQIGKAGLLMSALTVFILILYFVIDNFVINRRPWLPECTPIYIQYFVKFFIIGITVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQIPSPDVFLPKVLDLIVNGISINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEEKLYKVYTFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVRTVIEPMACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAIAKCKQAGITVRMVTGDNINTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTVGEHRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLKRRPYGRNKPLISRTMMKNILGHAFYQLIVIFILVFAGEKFFDIDSGRKAPLHSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFSGIYRNIIFCSVVLGTFICQIFIVEFGGKPFSCTSLSLSQWLWCLFIGIGELLWGQFISAIPTRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRRGQILWFRGLNRIQTQIDVINTFQTGASFKGVLRRQNMGQHLDVKLVPSSSYIKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETSV | |||||||
Modified residue | 13 | UniProt | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 13 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 17 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 238 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 328 | UniProt | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 328 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 570 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue | 1102 | UniProt | Phosphothreonine; by PKC | ||||
Sequence: T | |||||||
Modified residue (large scale data) | 1115 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 1149 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 1162 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 1165 | PRIDE | Phosphoserine | ||||
Sequence: S | |||||||
Modified residue (large scale data) | 1181 | PRIDE | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Gene expression databases
Organism-specific databases
Interaction
Subunit
Interacts with SLC35G1 and STIM1 (PubMed:22084111).
Interacts with calmodulin (PubMed:10493800, PubMed:2963820).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P23634 | CALCA P01258 | 2 | EBI-1174388, EBI-1018474 | |
BINARY | P23634 | RNLS Q5VYX0 | 3 | EBI-1174388, EBI-3386081 | |
BINARY | P23634 | TAS2R7 Q9NYW3 | 2 | EBI-1174388, EBI-21852300 | |
XENO | P23634-6 | Dlg2 Q63622 | 2 | EBI-1174437, EBI-396947 | |
XENO | P23634-6 | Dlg3 Q62936 | 2 | EBI-1174437, EBI-349596 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 294-310 | Basic and acidic residues | ||||
Sequence: EGEKKKKGKKQGVPENR | ||||||
Region | 294-318 | Disordered | ||||
Sequence: EGEKKKKGKKQGVPENRNKAKTQDG | ||||||
Region | 1086-1103 | Calmodulin-binding subdomain A | ||||
Sequence: LRRGQILWFRGLNRIQTQ | ||||||
Region | 1104-1113 | Calmodulin-binding subdomain B | ||||
Sequence: IDVINTFQTG |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 8 isoforms produced by Alternative splicing. There is a combination of two alternatively spliced domains at N-terminal site A (X and Z) and at C-terminal site B/C (A, B, D and K). The splice sites have mostly been studied independently. Full isoforms so far detected are isoform XA and isoform XB. Experimental confirmation may be lacking for some isoforms.
P23634-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameXD
- SynonymsAIICIV
- Length1,241
- Mass (Da)137,920
- Last updated2001-06-01 v2
- Checksum568544103CD5F494
P23634-2
- NameXA
- SynonymsAIICII
- Differences from canonical
- 1140-1241: IKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETSV → VAVAPVKSSPTTSVPAVSSPPMGNQSGQSVP
P23634-3
- NameZA
- SynonymsAICII
- Differences from canonical
- 301-312: Missing
- 1140-1241: IKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETSV → VAVAPVKSSPTTSVPAVSSPPMGNQSGQSVP
P23634-4
- NameXK
- SynonymsXG
- Differences from canonical
- 1009-1044: Missing
- 1140-1241: IKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETSV → VAVAPVKSSPTTSVPAVSSPPMGNQSGQSVP
P23634-5
- NameZK
- SynonymsZG
- Differences from canonical
- 301-312: Missing
- 1009-1044: Missing
- 1140-1241: IKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETSV → VAVAPVKSSPTTSVPAVSSPPMGNQSGQSVP
P23634-6
- NameXB
- SynonymsAIICI, hPMCA4b
- Differences from canonical
- 1104-1139: Missing
P23634-7
- NameZB
- SynonymsAICI
- Differences from canonical
- 301-312: Missing
- 1104-1139: Missing
P23634-8
- NameZD
- SynonymsAICIV
- Differences from canonical
- 301-312: Missing
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Features
Showing features for compositional bias, alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 294-310 | Basic and acidic residues | ||||
Sequence: EGEKKKKGKKQGVPENR | ||||||
Alternative sequence | VSP_000402 | 301-312 | in isoform ZA, isoform ZK, isoform ZB and isoform ZD | |||
Sequence: Missing | ||||||
Sequence conflict | 492 | in Ref. 3; CAD97686 | ||||
Sequence: S → C | ||||||
Alternative sequence | VSP_000403 | 1009-1044 | in isoform XK and isoform ZK | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_000404 | 1104-1139 | in isoform XB and isoform ZB | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_000405 | 1140-1241 | in isoform XA, isoform XK, isoform ZA and isoform ZK | |||
Sequence: IKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETSV → VAVAPVKSSPTTSVPAVSSPPMGNQSGQSVP | ||||||
Sequence conflict | 1144 | in Ref. 8; AA sequence | ||||
Sequence: K → N | ||||||
Sequence conflict | 1147 | in Ref. 8; AA sequence | ||||
Sequence: H → S | ||||||
Sequence conflict | 1153 | in Ref. 8; AA sequence | ||||
Sequence: S → F | ||||||
Sequence conflict | 1178 | in Ref. 9; AA sequence | ||||
Sequence: L → Q | ||||||
Sequence conflict | 1187 | in Ref. 9; AA sequence | ||||
Sequence: E → Q | ||||||
Sequence conflict | 1190 | in Ref. 3; CAD97686 | ||||
Sequence: E → G |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M25874 EMBL· GenBank· DDBJ | AAA50819.1 EMBL· GenBank· DDBJ | mRNA | ||
M83363 EMBL· GenBank· DDBJ | AAA36455.1 EMBL· GenBank· DDBJ | mRNA | ||
BX537444 EMBL· GenBank· DDBJ | CAD97686.1 EMBL· GenBank· DDBJ | mRNA | ||
U42026 EMBL· GenBank· DDBJ | AAB17577.1 EMBL· GenBank· DDBJ | mRNA | ||
U42061 EMBL· GenBank· DDBJ | AAB17578.1 EMBL· GenBank· DDBJ | mRNA | ||
U42062 EMBL· GenBank· DDBJ | AAB17579.1 EMBL· GenBank· DDBJ | mRNA | ||
U42378 EMBL· GenBank· DDBJ | AAB17580.1 EMBL· GenBank· DDBJ | mRNA | ||
AC114402 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AL513343 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CH471067 EMBL· GenBank· DDBJ | EAW91483.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CH471067 EMBL· GenBank· DDBJ | EAW91486.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC140774 EMBL· GenBank· DDBJ | AAI40775.1 EMBL· GenBank· DDBJ | mRNA |