P23532 · PTLA_LACLL
- ProteinPTS system lactose-specific EIIA component
- GenelacF
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids105 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II LacEF PTS system is involved in lactose transport.
Miscellaneous
This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.
Cofactor
Note: Binds 1 Mg2+ ion per trimer.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | metal ion binding | |
Molecular Function | transferase activity | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended namePTS system lactose-specific EIIA component
- Alternative names
Gene names
Encoded on
- Plasmid pLP712
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Streptococcaceae > Lactococcus
Accessions
- Primary accessionP23532
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 18 | No activity. | ||||
Sequence: G → E |
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000186597 | 1-105 | PTS system lactose-specific EIIA component | |||
Sequence: MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVVEAGSCIAEAHSSQTGMLAREASGEELPYSVTMMHGQDHLMTTILLKDVIHHLIELYKRGAK | ||||||
Modified residue | 78 | Phosphohistidine; by HPr | ||||
Sequence: H |
Keywords
- PTM
PTM databases
Expression
Induction
By lactose. The operon consists of lacABCDFEGX. A second transcript of only lacF and lacE is also lactose-induced.
Interaction
Subunit
Homotrimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 4-102 | PTS EIIA type-3 | ||||
Sequence: EEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVVEAGSCIAEAHSSQTGMLAREASGEELPYSVTMMHGQDHLMTTILLKDVIHHLIELYKR |
Domain
The PTS EIIA type-3 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-3 domain.
Family and domain databases
Sequence
- Sequence statusComplete
- Length105
- Mass (Da)11,448
- Last updated1991-11-01 v1
- ChecksumD2ACDEF3433A8C6B
Keywords
- Technical term