P23485 · FECR_ECOLI

  • Protein
    Ferric citrate uptake sigma factor regulator FecR
  • Gene
    fecR
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Required for transcriptional activation of the fecABCDE operon by sigma factor FecI (PubMed:2254251, PubMed:7752886).
Undergoes sequential cleavage to produce an N-terminal cytoplasmic fragment which is released from the membrane and binds to FecI, allowing it to activate transcription of the fecABCDE operon which mediates ferric citrate transport (PubMed:33865858).
In the absence of citrate, FecR inactivates FecI (PubMed:2254251).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentplasma membrane
Molecular Functionsigma factor antagonist activity
Molecular Functiontransmembrane signaling receptor activity
Biological Processintracellular iron ion homeostasis
Biological Processiron ion transport
Biological Processregulation of DNA-templated transcription initiation
Biological Processsignal transduction involved in regulation of gene expression

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ferric citrate uptake sigma factor regulator FecR

Gene names

    • Name
      fecR
    • Ordered locus names
      b4292, JW4252

Organism names

  • Taxonomic identifier
  • Strains
    • K12
    • K12 / MG1655 / ATCC 47076
    • K12 / W3110 / ATCC 27325 / DSM 5911
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P23485
  • Secondary accessions
    • Q2M623

Proteomes

Subcellular Location

Cell inner membrane
; Single-pass membrane protein
Note: Contains an internal twin-arginine motif immediately upstream of the transmembrane region which allows it to be translocated across the inner membrane via the Tat system.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-81Cytoplasmic
Transmembrane82-98Helical
Topological domain99-317Periplasmic

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis79-80Aberrant transcription of the fecABCDE operon, probably due to prevention of the periplasmic translocation of the C-terminal FecR domain which abrogates FecR binding to citrate-loaded FecA.

Miscellaneous

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000872181-317Ferric citrate uptake sigma factor regulator FecR

Post-translational modification

Sequentially cleaved starting with cleavage immediately after translation to generate CL(a). CL(a) is then cleaved by an unknown protease in the periplasm to yield CL(b). Finally, CL(b) undergoes intramembrane proteolysis by RseP to produce the N-terminal cytoplasmic fragment CL(c) which is released from the membrane and binds to sigma factor FecI, allowing it to activate transcription of the fecABCDE operon.

Proteomic databases

Expression

Induction

Induced by hydroxyurea.

Interaction

Subunit

Interacts (via periplasmic C-terminus) with FecA (via periplasmic N-terminus) (PubMed:10633096).
Interacts (via cytoplasmic N-terminus) with FecI (PubMed:10633096).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif79-80Internal twin-arginine motif; allows for targeting to the inner membrane via the Tat system

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    317
  • Mass (Da)
    35,532
  • Last updated
    1991-11-01 v1
  • Checksum
    798EC615815AF9D7
MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQDNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSETGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTAKDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNAGESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVAGLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M63115
EMBL· GenBank· DDBJ
AAA23767.1
EMBL· GenBank· DDBJ
Genomic DNA
U14003
EMBL· GenBank· DDBJ
AAA97188.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC77248.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAE78283.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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