P23204 · PPARA_MOUSE
- ProteinPeroxisome proliferator-activated receptor alpha
- GenePpara
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids468 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as a transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
Features
Showing features for dna binding, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 99-173 | Nuclear receptor | ||||
Sequence: NIECRICGDKASGYHYGVHACEGCKGFFRRTIRLKLVYDKCDRSCKIQKKNRNKCQYCRFHKCLSVGMSHNAIRF | ||||||
Site | 433 | Essential for heterodimerization with RXRA | ||||
Sequence: L |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePeroxisome proliferator-activated receptor alpha
- Short namesPPAR-alpha
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionP23204
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Involvement in disease
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 119 | Reduces DNA binding and strongly decreases transcriptional activation; when associated with A-122. | ||||
Sequence: C → A | ||||||
Mutagenesis | 122 | Reduces DNA binding and strongly decreases transcriptional activation; when associated with A-119. | ||||
Sequence: C → A | ||||||
Mutagenesis | 292 | No effect on interaction with PER2. | ||||
Sequence: K → A | ||||||
Mutagenesis | 459-468 | Slightly reduces interaction with PER2. | ||||
Sequence: Missing |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 26 variants from UniProt as well as other sources including ClinVar and dbSNP.
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000053482 | 1-468 | Peroxisome proliferator-activated receptor alpha | |||
Sequence: MVDTESPICPLSPLEADDLESPLSEEFLQEMGNIQEISQSIGEESSGSFGFADYQYLGSCPGSEGSVITDTLSPASSPSSVSCPVIPASTDESPGSALNIECRICGDKASGYHYGVHACEGCKGFFRRTIRLKLVYDKCDRSCKIQKKNRNKCQYCRFHKCLSVGMSHNAIRFGRMPRSEKAKLKAEILTCEHDLKDSETADLKSLGKRIHEAYLKNFNMNKVKARVILAGKTSNNPPFVIHDMETLCMAEKTLVAKMVANGVEDKEAEVRFFHCCQCMSVETVTELTEFAKAIPGFANLDLNDQVTLLKYGVYEAIFTMLSSLMNKDGMLIAYGNGFITREFLKNLRKPFCDIMEPKFDFAMKFNALELDDSDISLFVAAIICCGDRPGLLNIGYIEKLQEGIVHVLKLHLQSNHPDDTFLFPKLLQKMVDLRQLVTEHAQLVQVIKKTESDAALHPLLQEIYRDMY |
Proteomic databases
PTM databases
Expression
Tissue specificity
Highly expressed in liver, kidney and heart. Very weakly expressed in brain and testis.
Developmental stage
It appears first at 13.5 dpc and increases until birth.
Gene expression databases
Interaction
Subunit
Heterodimer; with RXRA. This heterodimerization is required for DNA binding and transactivation activity. Interacts with NCOA3 coactivator. Interacts with CITED2; the interaction stimulates its transcriptional activity. Also interacts with PPARBP in vitro. Interacts with AKAP13, LPIN1, PRDM16 and coactivator NCOA6. Interacts with ASXL1 and ASXL2. Interacts with PER2. Interacts with SIRT1; the interaction seems to be modulated by NAD+ levels (By similarity).
Interacts with CRY1 and CRY2 (PubMed:28683290).
Interacts with CRY1 and CRY2 (PubMed:28683290).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P23204 | Men1 O88559 | 2 | EBI-5273083, EBI-3990176 |
Protein-protein interaction databases
Chemistry
Miscellaneous
Structure
Family & Domains
Features
Showing features for zinc finger, domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 102-122 | NR C4-type | ||||
Sequence: CRICGDKASGYHYGVHACEGC | ||||||
Zinc finger | 139-161 | NR C4-type | ||||
Sequence: CDRSCKIQKKNRNKCQYCRFHKC | ||||||
Domain | 239-466 | NR LBD | ||||
Sequence: FVIHDMETLCMAEKTLVAKMVANGVEDKEAEVRFFHCCQCMSVETVTELTEFAKAIPGFANLDLNDQVTLLKYGVYEAIFTMLSSLMNKDGMLIAYGNGFITREFLKNLRKPFCDIMEPKFDFAMKFNALELDDSDISLFVAAIICCGDRPGLLNIGYIEKLQEGIVHVLKLHLQSNHPDDTFLFPKLLQKMVDLRQLVTEHAQLVQVIKKTESDAALHPLLQEIYRD | ||||||
Region | 304-433 | Required for heterodimerization with RXRA | ||||
Sequence: DQVTLLKYGVYEAIFTMLSSLMNKDGMLIAYGNGFITREFLKNLRKPFCDIMEPKFDFAMKFNALELDDSDISLFVAAIICCGDRPGLLNIGYIEKLQEGIVHVLKLHLQSNHPDDTFLFPKLLQKMVDL |
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length468
- Mass (Da)52,347
- Last updated1996-10-01 v2
- Checksum2930A5191C610B6B
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 75 | in Ref. 1; CAA40856 | ||||
Sequence: A → R |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X57638 EMBL· GenBank· DDBJ | CAA40856.1 EMBL· GenBank· DDBJ | mRNA | ||
X75289 EMBL· GenBank· DDBJ | CAA53042.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X75290 EMBL· GenBank· DDBJ | CAA53042.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X75291 EMBL· GenBank· DDBJ | CAA53042.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X75292 EMBL· GenBank· DDBJ | CAA53042.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X75293 EMBL· GenBank· DDBJ | CAA53042.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X75294 EMBL· GenBank· DDBJ | CAA53042.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BC016892 EMBL· GenBank· DDBJ | AAH16892.1 EMBL· GenBank· DDBJ | mRNA | ||
X89577 EMBL· GenBank· DDBJ | CAA61754.1 EMBL· GenBank· DDBJ | mRNA |